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Comparative genetic diversity of wild and hatchery-produced Pacific oyster (Crassostrea gigas) populations in Korea using multiplex PCR assays with nine polymorphic microsatellite markers

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Abstract

The Pacific oyster, Crassostrea gigas, is the most important and valuable commercial fishery species in Korea. Its farming started 20 years ago and is still rapid expansion in Korea. In this study, to maintain the genetic diversity of this valuable marine resource, possible genetic similarity and differences between the wild population and hatchery population in Tongyeong, Korea were accessed using multiplex assays with nine highly polymorphic microsatellite loci. A total of 250 different alleles were found over all loci. Despite a long history of hatchery practices, very high levels of polymorphism (mean alleles = 22.89 and mean heterozygosity = 0.92) were detected between the two populations. No statistically significant reductions were found in heterozygosity or allelic diversity in the hatchery population compared with the wild population. However, significant genetic heterogeneity was found between two populations. These results provide no evidence to show that hatchery practice of Pacific oyster in Korea has significantly affected the genetic variability of the hatchery stock. Although further studies are needed for comprehensive determinations of the hatchery and wild populations with increased number of Pacific oyster sample collections, information on the genetic variation and differentiation obtained in this study can be applied for genetic monitoring of aquaculture stocks, genetic improvement by selective breeding and designing of more efficient conservation management guidelines for these valuable genetic materials.

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Acknowledgments

This work was funded by a Grant from the National Fisheries Research and Development Institute (NFRDI; contribution number RP-2013-BT-073). The views expressed herein are those of the authors and do not necessarily reflect the views of NFRDI.

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Authors declare no conflict of interest on this article contents.

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Authors and Affiliations

  1. Biotechnology Research Division, National Fisheries Research and Development Institute, Pusan, 619-705, Korea

    Hye Suck An, Jang Wook Lee, Woo Jin Kim, Eun Mi Kim, Jung Youn Park, Jeong In Myeong & Chul Min An

  2. West Sea Fisheries Research Institute, National Fisheries Research and Development Institute, Inchon, 400-420, Korea

    Hyun Jeong Lim, Soon Gyu Byun & Young Baek Hur

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  1. Hye Suck An
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  2. Jang Wook Lee
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  3. Woo Jin Kim
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  4. Hyun Jeong Lim
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  5. Eun Mi Kim
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  6. Soon Gyu Byun
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  7. Young Baek Hur
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  10. Chul Min An
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Correspondence to Hye Suck An.

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An, H.S., Lee, J.W., Kim, W.J. et al. Comparative genetic diversity of wild and hatchery-produced Pacific oyster (Crassostrea gigas) populations in Korea using multiplex PCR assays with nine polymorphic microsatellite markers. Genes Genom 35, 805–815 (2013). https://doi.org/10.1007/s13258-013-0140-2

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  • DOI: https://doi.org/10.1007/s13258-013-0140-2

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