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Characterization of 20 microsatellite loci by multiplex PCR in swimming crab, Portunus trituberculatus

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Abstract

The swimming crab, Portunus trituberculatus (Crustacea: Decapoda: Brachyura), is one of the most important fishery resources in East Asia. The aim of this work is to develop microsatellite multiplex PCR systems for 20 microsatellites which were isolated from a microsatellite-enriched genomic library and to explore the genetic diversity of P. trituberculatus captured in the coastal regions of the Yellow Sea in Korea. We prepared four multiplex PCR systems (two hexplex and tetraplex systems each) which could be used for the genotyping of 20 markers. Most markers in this system are highly informative with mean polymorphism information contents and observed heterozygosity of 0.913 and 0.930. The total number of alleles observed in this study was 724, and the mean allelic number per locus was 36.2. Significant deviations from the Hardy–Weinberg equilibrium (HWE) were observed at six loci in the Wonsando Island population, but all the loci deviated from the HWE in the Jeonjangpo population. The genetic differentiation between the two populations and the phylogenetic tree constructed by the unweighted pair group method with the arithmetic average suggested that there is no significant genetic difference between Wonsando Island and Jeonjangpo populations. The prepared multiplex PCR systems and population genetic data will be helpful to study phylogeographic analysis as well as to prepare strategies of stock management and aquaculture of P. trituberculatus.

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Acknowledgments

We thank Dr. Hyung Chul Seo in the National Fisheries Research and Development Institute and Dr. Sung Il Hwang in the Institute of Subaqueous Ecotechnology collecting crab samples. This study was supported by the Korea Fisheries Resources Agency (FIRA), Ministry for Food, Agriculture, Forestry and Fisheries, and the National Research Foundation (NRF) grant funded by the Ministry of Education, Science and Technology, Republic of Korea (2011-0013694).

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Authors and Affiliations

  1. Department of Biological Science, Kongju National University, 182 Sinkwan-dong, Gongju, 314-701, Chungnam, South Korea

    Hye Jin Lee, Young Se Hyun & Ki Wha Chung

  2. Korea Fisheries Resources Agency, Busan, 612-020, South Korea

    Do-Hyun Lee, Seung-Jong Yoon, Dae Hyun Kim & Sang-Gyu Kim

  3. Department of Biological Sciences, Inha University, 253 Yonghyun-dong, Nam-gu, Incheon, 402-751, South Korea

    Gi-Sik Min

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  1. Hye Jin Lee
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  2. Do-Hyun Lee
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  3. Seung-Jong Yoon
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  4. Dae Hyun Kim
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  5. Sang-Gyu Kim
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  6. Young Se Hyun
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  7. Gi-Sik Min
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  8. Ki Wha Chung
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Correspondence to Ki Wha Chung.

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Hye Jin Lee and Do-Hyun Lee contributed equally to this study.

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Lee, H.J., Lee, DH., Yoon, SJ. et al. Characterization of 20 microsatellite loci by multiplex PCR in swimming crab, Portunus trituberculatus . Genes Genom 35, 77–85 (2013). https://doi.org/10.1007/s13258-013-0062-z

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  • DOI: https://doi.org/10.1007/s13258-013-0062-z

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