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In vivo bone regeneration ability of different layers of natural silk cocoon processed using an eco-friendly method

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Abstract

Silk cocoons are primarily composed of the proteins fibroin and sericin. To achieve guided bone regeneration (GBR), we have developed a simple and ecofriendly processing technique to obtain microperforated thin membranes from cocoons. The separated silk membranes composed of both fibroin and sericin were classified by the cocoon layers (i.e., inner, middle, or outer) from which they originated. This report details the biological properties and the cellular responses of the three silk layers. The different cocoon layers were compared for their bone regeneration capabilities in vivo. The porosity of the silk nets increased from the inner layer to the outer layer when all of the membranes were compared using scanning electron microscopy (SEM). A difference in spectral intensity was observed in the Fourier transform infrared (FT-IR) spectra, indicating different amino acid compositions in these layers. An amino acid composition test demonstrated that the serine content decreased from the outer layer to the inner layer. Characterization of the protein release from each net demonstrated that the highest amount of protein release was observed in the inner layer group. The middle layer showed higher alkaline phosphatase (ALP) activity than the other layers in cellular experiments. Animal experiments indicated that the middle layers exhibit the highest bone volume 8 weeks post-operation (p<0.05). The membranes obtained directly from the thin middle layer of silk cocoons without any regeneration protocol have the potential to be used as an eco-friendly bone regeneration material for in vivo applications.

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Author information

Authors and Affiliations

  1. Sericultural and Apicultural Materials Division, National Institute of Agricultural Science, Wanju-gun, Jeonbuk, 55365, Korea

    HaeYong Kweon & You-Young Jo

  2. Department of Oral and Maxillofacial Surgery, Chungbuk National University Hospital, Cheongju, Chungbuk, 28644, Korea

    Hyun Seok

  3. Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University, Gangneung, Gangwon, 25457, Korea

    Seong-Gon Kim

  4. Daegu Center, Korea Basic Science Institute, Daegu, 41566, Korea

    Weon-Sik Chae

  5. Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal, 721302, India

    Sunaina Sapru & Subhas C. Kundu

  6. Department of Oral Biochemistry, College of Dentistry, Gangneung-Wonju National University, Gangneung, Gangwon, 25457, Korea

    Dae-Won Kim

  7. Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute, Skeletal Diseases Genome Research Center, BK21 Plus KNU Biomedical Convergence Program, Kyungpook National University School of Medicine, Daegu, 41944, Korea

    Na-Rae Park, Xiangguo Che & Je-Yong Choi

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  1. HaeYong Kweon
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  2. You-Young Jo
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  3. Hyun Seok
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  4. Seong-Gon Kim
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  5. Weon-Sik Chae
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  6. Sunaina Sapru
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  7. Subhas C. Kundu
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  11. Je-Yong Choi
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Corresponding author

Correspondence to Seong-Gon Kim.

Additional information

Acknowledgments: This work was carried out with the support of the “Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01121404)”, Rural Development Administration, Republic of Korea.

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Kweon, H., Jo, YY., Seok, H. et al. In vivo bone regeneration ability of different layers of natural silk cocoon processed using an eco-friendly method. Macromol. Res. 25, 806–816 (2017). https://doi.org/10.1007/s13233-017-5085-x

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  • DOI: https://doi.org/10.1007/s13233-017-5085-x

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