Skip to main content
SpringerLink
Log in

Comparison of the physical properties and in vivo bioactivities of silkworm-cocoon-derived silk membrane, collagen membrane, and polytetrafluoroethylene membrane for guided bone regeneration

  • Articles
  • Published:
Macromolecular Research Aims and scope Submit manuscript

Abstract

The aim of this study was to evaluate silkworm-cocoon-derived silk membrane (SM) for application in guided bone regeneration (GBR) and to compare it with commercially available GBR membranes, such as collagen membrane (CM) and polytetrafluoroethylene membrane (PM). The SM, CM, and PM membranes were examined via scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) analysis, and tensile strength evaluation. Thirty rats were used for the experiments. Critical-sized parietal bone defects (diameter: 8.0 mm) were divided into three groups according to the applied GBR membrane. The rats were sacrificed 4 or 8 weeks after surgery and evaluated by micro-computerized tomography (μ-CT) and histology. The FTIR analysis of the SM was similar to that of the CM. The SEM images showed that the surface characteristics of the SM differed from those of the CM and PM. The SM showed a complex network structure of silk fibers. The wet SM was also more resistant to tensile stress than the wet CM and PM. The μ-CT results of the animal study showed that the bone volume of the SM group was higher than those of the CM and PM groups 4 weeks after the operation (p<0.05). The histological analysis showed that the new bone regeneration of the SM group was higher than those of the CM and PM groups 4 and 8 weeks after the operation (p<0.05). This study demonstrated that the tensile strength of the wet SM was higher than the tensile strength of the wet CM and PM groups. The bone formation of the SM group was higher than those of the other two groups.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. M. C. Bottino, V. Thomas, G. Schmidt, Y. K. Vohra, T. M. Chu, M. J. Kowolik, and G. M. Janowski, Dent. Mater., 28, 703 (2012).

    Article  CAS  Google Scholar 

  2. P. Bunyaratavej and H. L. Wang, J. Periodontol., 72, 215 (2001).

    Article  CAS  Google Scholar 

  3. E. E. Machtei, J. Periodontol., 72, 512 (2001).

    Article  CAS  Google Scholar 

  4. D. N. Tatakis, A. Promsudthi, and U. M. Wikesjö, Periodontol. 2000, 19, 59 (1999).

    Article  CAS  Google Scholar 

  5. S. Zahedi, R. Legrand, G. Brunel, A. Albert, W. Dewe, B. Coumans, and J. P. Bernard, J. Periodontol., 69, 1238 (1998).

    Article  CAS  Google Scholar 

  6. D. Rothamel, F. Schwarz, M. Sager, M. Herten, A. Sculean, and J. Becker, Clin. Oral Implants Res., 16, 369 (2005).

    Article  Google Scholar 

  7. D. P. Speer, M. Chvapil, C. D. Eskelson, and J. Ulreich, J. Biomed. Mater. Res., 14, 753 (1980).

    Article  CAS  Google Scholar 

  8. S. Zhao, E. M. Pinholt, J. E. Madsen, and K. Donath, J. Craniomaxillofac. Surg., 28, 116 (2000).

    Article  CAS  Google Scholar 

  9. C. S. Ki, Y. H. Park, and H. J. Jin, Macromol. Res., 17, 935 (2009).

    Article  CAS  Google Scholar 

  10. W. Zaoming, R. Codina, E. Fernandez-Caldas, and R. F. Lockey, J. Investig. Allergol. Clin. Immunol., 6, 237 (1996).

    CAS  Google Scholar 

  11. K. H. Kim, L. Jeong, H. N. Park, S. Y. Shin, W. H. Park, S. C. Lee, T. I. Kim, Y. J. Park, Y. J. Seol, Y. M. Lee, Y. Ku, I. C. Rhyu, S. B. Han, and C. P. Chung, J. Biotechnol., 120, 327 (2005).

    Article  CAS  Google Scholar 

  12. G. H. Altman, F. Diaz, C. Jakuba, T. Calabro, R. L. Horan, J. Chen, H. Lu, J. Richmond, and D. L. Kaplan, Biomaterials, 24, 401 (2003).

    Article  CAS  Google Scholar 

  13. H. Yoon, E. Y. Kim, H. Kim, C. H. Park, C. K. Joo, and G. Khang, Macromol. Res., 22, 297 (2014).

    Article  CAS  Google Scholar 

  14. S. W. Lee, S. G. Kim, J. Y. Song, H. Kweon, Y. Y. Jo, K. G. Lee, S. W. Kang, and B. E. Yang, J. Craniofac. Surg., 24, 1927 (2013).

    Article  Google Scholar 

  15. J. Y. Song, S. G. Kim, J. W. Lee, W. S. Chae, H. Kweon, Y. Y. Jo, K. G. Lee, Y. C. Lee, J. Y. Choi, and J. Y. Kim, Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod., 112, e26 (2011).

    Article  Google Scholar 

  16. P. Aramwit, S. Kanokpanont, W. De-Eknamkul, and T. Srichana, J. Biosci. Bioeng., 107, 556 (2009).

    Article  CAS  Google Scholar 

  17. P. Aramwit, P. Towiwat, and T. Srichana, Nat. Prod. Commun., 8, 501 (2013).

    CAS  Google Scholar 

  18. B. Panilaitis, G. H. Altman, J. Chen, H. J. Jin, V. Karageorgiou, and D. L. Kaplan, Biomaterials, 24, 3079 (2003).

    Article  CAS  Google Scholar 

  19. F. Chen, D. Porter, and F. Vollrath, Acta Biomater., 8, 2620 (2012).

    Article  CAS  Google Scholar 

  20. J. H. Kim, S. H. Oh, and J. H. Lee, Biomater. Res., 10, 55 (2006).

    CAS  Google Scholar 

  21. S. R. Thaller, A. Dart, and H. Tesluk, Ann. Plast. Surg., 31, 429 (1993).

    Article  CAS  Google Scholar 

  22. P. P. Spicer, J. D. Kretlow, S. Young, J. A. Jansen, F. K. Kasper, and A. G. Mikos, Nat. Protoc., 7, 1918 (2012).

    Article  CAS  Google Scholar 

  23. D. Lundgren, S. Nyman, T. Mathisen, S. Isaksson, and B. Klinge, J. Craniomaxillofac. Surg., 20, 257 (1992).

    Article  CAS  Google Scholar 

  24. L. Dupoirieux, D. Pourquier, M. C. Picot, and M. Neves, Int. J. Oral Maxillofac. Surg., 30, 58 (2001).

    Article  CAS  Google Scholar 

  25. A. Jan, G. K. Sándor, B. B. Brkovic, S. Peel, A. W. Evans, and C. M. Clokie, Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod., 107, 157 (2009).

    Article  Google Scholar 

  26. A. Odgaard, E. B. Jensen, and H. J. Gundersen, J. Microsc., 157, 149 (1990).

    Article  CAS  Google Scholar 

  27. A. Odgaard, Bone, 20, 315 (1997).

    Article  CAS  Google Scholar 

  28. D. Foschi, F. Corsi, P. Cellerino, A. Rizzi, E. Morandi, and E. Trabucchi, Eur. Surg. Res., 33, 16 (2001).

    Article  CAS  Google Scholar 

  29. J. Street, M. Bao, L. de Guzman, S. Bunting, F. V. Peale Jr, N. Ferrara, H. Steinmetz, J. Hoeffel, J. L. Cleland, A. Daugherty, N. van Bruggen, H. P. Redmond, and R. A. Carano, Proc. Natl. Acad. Sci. U.S.A., 99, 9656 (2002).

    Article  CAS  Google Scholar 

  30. Y. J. Chen, T. Wurtz, C. J. Wang, Y. R. Kuo, K. D. Yang, H. C. Huang, and F. S. Wang, J. Orthop. Res., 22, 526 (2004).

    Article  CAS  Google Scholar 

  31. R. E. Unger, A. Sartoris, K. Peters, A. Motta, C. Migliaresi, M. Kunkel, U. Bulnheim, J. Rychly, and C. J. Kirkpatrick, Biomaterials, 28, 3965 (2007).

    Article  CAS  Google Scholar 

  32. K. Tsubouchi, Y. Igarashi, Y. Takasu, and H. Yamada, Biosci. Biotechnol. Biochem., 69, 403 (2005).

    Article  CAS  Google Scholar 

  33. B. B. Mandal and S. C. Kundu, Biomaterials, 30, 2956 (2009).

    Article  CAS  Google Scholar 

  34. D. Rothamel, F. Schwarz, A. Sculean, M. Herten, W. Scherbaum, and J. Becker, Clin. Oral Implants Res., 15, 443 (2004).

    Article  Google Scholar 

  35. T. Takata, H. L. Wang, and M. Miyauchi, J. Periodont. Res., 36, 322 (2001).

    Article  CAS  Google Scholar 

  36. S. Keten, Z. Xu, B. Ihle, and M. J. Buehler, Nat. Mater., 9, 359 (2010).

    Article  CAS  Google Scholar 

  37. Y. Cao and B. Wang, Int. J. Mol. Sci., 10, 1514 (2009).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University, Gangwon, 210-702, Korea

    Yong-Yun Ha, Young-Wook Park & Seong-Gon Kim

  2. Sericultural and Apicultural Materials Division, National Academy of Agricultural Science, Gyeonggi, 441-707, Korea

    HaeYong Kweon & You-Young Jo

Authors
  1. Yong-Yun Ha
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Young-Wook Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. HaeYong Kweon
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. You-Young Jo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Seong-Gon Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Seong-Gon Kim.

Additional information

The image from this article is used as the cover image of the Volume 22, Issue 9.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ha, YY., Park, YW., Kweon, H. et al. Comparison of the physical properties and in vivo bioactivities of silkworm-cocoon-derived silk membrane, collagen membrane, and polytetrafluoroethylene membrane for guided bone regeneration. Macromol. Res. 22, 1018–1023 (2014). https://doi.org/10.1007/s13233-014-2138-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13233-014-2138-2

Keywords

Search

Navigation