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Characterization of fungal pathogens associated with grapevine trunk diseases in Arkansas and Missouri

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Abstract

Grapevine trunk diseases are a major concern to the wine- and table-grape industries worldwide, limiting both vineyard longevity and productivity. Field surveys conducted throughout the grape-growing regions of Arkansas and Missouri revealed the presence of three economically important grapevine trunk diseases including, Botryosphaeria canker, Eutypa dieback and esca. Morphological studies along with multi-gene phylogenetical analyses confirmed the identification of 15 different fungal taxa associated with different vascular symptoms. These include Botryosphaeria dothidea, Diatrypella sp., Diplodia seriata, Dothiorella americana, Eutypa lata, Eutypella vitis, Lasiodiplodia missouriana, Lasiodiplodia viticola, Neofusicoccum ribis, Neofusicoccum vitifusiforme, Pestalotiopsis sp., Pestalotiopsis uvicola, Phaeomoniella chlamydospora, Phomopsis viticola, Schyzophyllum commune, and Togninia minima. All of these represent new records on grapevines in Arkansas and Missouri. Dothiorella americana, L. missouriana and L. viticola are described as new species, and both N. ribis and N. vitifusiforme are first reported as grapevine pathogens in North America. Koch’s postulates confirmed the pathogenicity of all fungal species except S. commune in the interspecific hybrids Vignoles, Chambourcin, Norton, and Traminette. Lasiodiplodia spp., N. ribis, and P. viticola were the most virulent fungi, while D. americana, E. vitis and N. vitifusiforme were considered to be weak pathogens. This research highlights the importance that grapevine trunk diseases have on grapevine health in growing regions where, due to different climatological conditions, interspecific hybrid cultivars are predominantly grown.

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Acknowledgments

This work was partially funded by a grant from the Missouri Wine and Grape Board. The authors thank E. Bergmeier and A. Allen (Institute for Continental Climate Viticulture & Enology, University of Missouri, Columbia, Missouri) for providing the plant material for the pathogenicity assays and valuable pictures of the different grapevine symptoms, respectively.

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Authors and Affiliations

  1. Pacific Agri-Food Research Centre, 4200 Highway 97, Box 5000, Summerland, BC, V0H1Z0, Canada

    Jose Ramon Urbez-Torres

  2. Department of Plant Pathology, University of California Davis, Davis, CA, 95616, USA

    Francesca Peduto & W. D. Gubler

  3. Institute for Continental Climate Viticulture & Enology, University of Missouri, Columbia, MO, 65211, USA

    R. K. Striegler

  4. Department of Plant Pathology, University of Arkansas, Fayetteville, AR, 72701, USA

    K. E. Urrea-Romero, J. C. Rupe & R. D. Cartwright

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  1. Jose Ramon Urbez-Torres
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  2. Francesca Peduto
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  3. R. K. Striegler
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  4. K. E. Urrea-Romero
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  5. J. C. Rupe
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  7. W. D. Gubler
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Correspondence to W. D. Gubler.

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Urbez-Torres, J.R., Peduto, F., Striegler, R.K. et al. Characterization of fungal pathogens associated with grapevine trunk diseases in Arkansas and Missouri. Fungal Diversity 52, 169–189 (2012). https://doi.org/10.1007/s13225-011-0110-4

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