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Endophytic Colletotrichum from tropical grasses with a new species C. endophytica

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Abstract

Endophytic fungi are a diverse and important group of microorganisms. We investigated the occurrence of Colletotrichum species as endophytes in two common tropical grass species, Pennisetum purpureum (dwarf napier) and Cymbopogon citratus (lemon grass) in Thailand. Combined phylogenetic analysis of ITS, partial sequences of actin (ACT), calmodulin (CAL) and glyceraldehydes-3-phosphate dehydrogenase (GAPDH) gene regions and morphology were used to characterize the species. This is the first report of an association as endophytes of Colletotrichum fructicola, C. tropicale and C. siamense with Pennisetum purpureum, and C. fructicola and C. siamense with Cymbopogon citratus. Colletotrichum endophytica sp. nov. associated with Pennisetum purpureum, is introduced based on multi-locus phylogenetic analysis with descriptions and illustrations. The potential hyperdiversity of the endophytic Colletotrichum species associated with tropical grasses is discussed with an emphasis on future research.

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Acknowledgments

Dimuthu S. Manamgoda thanks Mushroom Research Foundation, Thailand and the State Key Laboratory of Mycology, Chinese Academy of Science, Beijing, China for Postgraduate Scholarships. K.D. Hyde thanks the National Research Council of Thailand, Colletotrichum grant number 54201020003 and King Abdulaziz City of Science and Technology, Riyadh, Saudi Arabia, project No. 10-Bio-965-02 to study Colletotrichum. L. Cai acknowledges grants NSFC 31070020 and CASKSCX2-YW-Z-1026. Rungtiwa Phookamsak and Fang Liu are thanked for laboratory assistance. We are very grateful to Dr. Roger G. Shivas, who gave valuable suggestions to this work during his academic visit to China (funded by NSFC 31110103906).

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Authors and Affiliations

  1. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No 3 1st West Beichen Road, Chaoyang District, Beijing, 100101, People’s Republic of China

    Dimuthu S. Manamgoda, Dhanushka Udayanga & Lei Cai

  2. Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand

    Dimuthu S. Manamgoda, Dhanushka Udayanga, Ekachai Chukeatirote & Kevin D. Hyde

  3. School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand

    Dimuthu S. Manamgoda, Dhanushka Udayanga, Ekachai Chukeatirote & Kevin D. Hyde

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  1. Dimuthu S. Manamgoda
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  2. Dhanushka Udayanga
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  3. Lei Cai
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  4. Ekachai Chukeatirote
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  5. Kevin D. Hyde
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Correspondence to Lei Cai.

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Manamgoda, D.S., Udayanga, D., Cai, L. et al. Endophytic Colletotrichum from tropical grasses with a new species C. endophytica . Fungal Diversity 61, 107–115 (2013). https://doi.org/10.1007/s13225-013-0256-3

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