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Isolation and characterization of a lead (Pb) tolerant Pseudomonas aeruginosa strain HF5 for decolorization of reactive red-120 and other azo dyes

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Abstract

Presence of heavy metals including lead (Pb) in the textile effluents is a crucial factor affecting the growth and potential of the dye decolorizing bacterial strains. This work was planned to isolate and characterize a bacterial strain exhibiting the potential to decolorize a range of azo dyes as well as the resistance to Pb. In this study, several Pb tolerant bacteria were isolated from effluents of textile industry. These bacterial isolates were screened for their potential of decolorizing the reactive red-120 (RR120) azo dye with presence of Pb (50 mg L−1). The most efficient isolate was further characterized for its potential to resist Pb and decolorize different azo dyes under varying cultural and incubation conditions. Out of the total 82 tested bacterial isolates, 30 bacteria were found to have varying potentials to resist the presence of lead (Pb) and carry out decolorization of an azo dye reactive red-120 (RR120) in the medium amended with Pb (50 mg L−1). The most efficient selected bacterium, Pseudomonas aeruginosa strain HF5, was found to show a good potential not only to grow in the presence of considerable concentration of Pb but also to decolorize RR120 and other azo dyes in the media amended with Pb. The strain HF5 completely (> 90%) decolorized RR120 in mineral salt medium amended with 100 mg L−1 of Pb and 20 g L−1 NaCl. This strain also considerably (> 50%) decolorized RR120 up to the presence of 2000 mg L−1 of Pb and 50 g L−1 of NaCl but with reduced rate. The optimal decolorization of RR120 by HF5 was achieved when the pH of the Pb amended (100 mg L−1) mineral salt media was adjusted at 7.5 and 8.5. Interestingly, this strain also showed the tolerance to a range of metal ions with varying MIC values. The Pseudomonas aeruginosa strain HF5 harboring the unique potentials to grow and decolorize the azo dyes in the presence of Pb is envisaged as a potential bioresource for devising the remediation strategies for treatment of colored textile wastewaters loaded with Pb and other heavy metal ions.

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Funding

This research work was funded by Government College University, Faisalabad, from its own resources under a GCUF2012 grant.

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Author notes

  1. Farhan Hafeez and Huma Farheen contributed equally to this work.

Authors and Affiliations

  1. Department of Environmental Sciences, COMSATS University Islamabad, Abbottabad Campus, Tobe Camp University Road, Abbottabad, 22060, Pakistan

    Farhan Hafeez & Muhammad Iqbal

  2. Department of Environmental Sciences & Engineering, Government College University, Allama Iqbal Road, Faisalabad, 38000, Pakistan

    Huma Farheen, Faisal Mahmood, Tanvir Shahzad & Sabir Hussain

  3. Department of Bioinformatics and Biotechnology, Government College University, Allama Iqbal Road, Faisalabad, 38000, Pakistan

    Muhammad Shahid

  4. Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan

    Sumaira Rasul & Hamid Manzoor

Authors
  1. Farhan Hafeez
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  2. Huma Farheen
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  3. Faisal Mahmood
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  4. Tanvir Shahzad
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  5. Muhammad Shahid
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  6. Muhammad Iqbal
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  7. Sumaira Rasul
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  8. Hamid Manzoor
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  9. Sabir Hussain
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Correspondence to Sabir Hussain.

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Hafeez, F., Farheen, H., Mahmood, F. et al. Isolation and characterization of a lead (Pb) tolerant Pseudomonas aeruginosa strain HF5 for decolorization of reactive red-120 and other azo dyes. Ann Microbiol 68, 943–952 (2018). https://doi.org/10.1007/s13213-018-1403-6

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  • DOI: https://doi.org/10.1007/s13213-018-1403-6

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