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Comparative secretome analysis of Fusarium sp. Q7-31T during liquid fermentation using oat straw as a carbon source

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Abstract

Q7-31T was screened from seven strains with high plant cell wall (PCW)-degrading enzyme activities and identified as Fusarium sp. based on the morphological characteristics and internal transcribed spacer (ITS) rDNA sequence analysis. The protein composition of the secretome produced by Q7-31T grown in a liquid medium with oat straw powders or glucoses as a carbon source was investigated using two-dimensional electrophoresis coupled with tandem mass spectrometry. A total of 28 protein species were identified among 115 spots that only existed or had a larger quantity (threefold or more) in the induction medium. As expected, most of them were potentially involved in PCW degradation. Six different superfamilies of glucoside hydrolases (GH5, GH7, GH10, GH13, GH18, and PL1) were identified, and proteases, oxidases, reductases, phospholipases, transferases, and proteins with unknown functions were also detected.

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References

  • Abbas A, Koc H, Liu F, Tien M (2005) Fungal degradation of wood: initial proteomic analysis of extracellular proteins of Phanerochaete chrysosporium grown on oak Substrate. Curr Genet 47:49–56

    Article  CAS  PubMed  Google Scholar 

  • Adav SS, Chao LT, Sze SK (2012) Quantitative secretomic analysis of Trichoderma reesei strains reveals enzymatic composition for lignocellulosic biomass degratation. Am Soc Biochem Mol Biol 11:1–15

    Google Scholar 

  • Carberry S, Doyle S (2007) Proteomic studies in biomedically and industrially relevant fungi. Cytotechnology 53:95–100

  • Cázares-García SV, Vázquez-Garcidueñas MS, Vázquez-Marrufo G (2013) Structural and phylogenetic analysis of laccases from Trichoderma: a bioinformatics approach. PLoS ONE 8:e55295

    Article  PubMed Central  PubMed  Google Scholar 

  • De Almeida MN, Guimarães VM, Bischoff KM, Falkoski DL, Pereira OL, Gonçalves DSPO, De Rezende ST (2011) Cellulases and hemicellulases from endophytic Acremonium species and its application on sugarcane bagasse hydrolysis. Appl Biochem Biotechnol 165:594–610

    Article  PubMed  Google Scholar 

  • De Moreno M, Smith J, Smith R (1985) Silver staining of protein in polyacrylamide gel increased sensitivity through a coomassie blue-silver stain procedure. Anal Biochem 151:466–470

  • Druzhinina IS, Shelest E, Kubicek CP (2012) Novel traits of Trichoderma predicted through the analysis of its secretome. FEMS Microbiol Lett 337:1–9

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Eveleigh D, Mandels M, Andreotti R, Roche C (2009) Measurement of saccharifying cellulase. Biotechnol Biofuels 2:21–23

    Article  PubMed Central  PubMed  Google Scholar 

  • Fryksdale BG, Jedrzejewski PT, Wong DL, Gaertner AL, Miller BS (2002) Impact of deglycosylation methods on two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization–time of flight-mass spectrometry for proteomic analysis. Electrophoresis 23:2184–2193

    Article  CAS  PubMed  Google Scholar 

  • Gitau EN, Kokwaro GO, Newton CR, Ward SA (2011) Global proteomic analysis of plasma from mice infected with Plasmodium berghei ANKA using two dimensional gel electrophoresis and matrix assisted laser desorption ionization-time of flight mass spectrometry. Malar J 10:205–213

  • Gomez-Gomez E, Isabel M, Roncero G, Di Pietro A, Hera C (2001) Molecular characterization of a novel endo-beta-1,4-xylanase gene from the vascular wilt fungus Fusarium oxysporum. Curr Genet 40:268–275

  • Herpoël-Gimbert I, Margeot A, Dolla A, Jan G, Mollé D, Lignon S, Mathis H, Sigoillot JC, Monot F, Asther M (2008) Comparative secretome analyses of two Trichoderma reesei RUT-C30 and CL847 hypersecretory strains. Biotechnol Biofuels 1:18

    Article  PubMed Central  PubMed  Google Scholar 

  • Himmel ME, Xu Q, Luo Y, Ding S-Y, Lamed R, Bayer EA (2010) Microbial enzyme systems for biomass conversion: emerging paradigms. Biofuels 1:323–341

    Article  CAS  Google Scholar 

  • Jiang L, He L, Fountoulakis M (2004) Comparison of protein precipitation methods for sample preparation prior to proteomic analysis. J Chromatogr A 20041023:317–320

    Article  Google Scholar 

  • Koc EC, Burkhart W, Blackburn K, Moyer MB, Schlatzer DM, Moseley A, Spremulli LL (2001) The large subunit of the mammalian mitochondrial ribosome-analysis of the complement of ribosomal proteins present. J Biol Chem 276:43958–43969

    Article  CAS  PubMed  Google Scholar 

  • Kuhad RC, Gupta R, Singh A (2011) Microbial cellulases and their industrial applications. Enzym Res 2011:280696

    Article  Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin-Phenol reagent. J Biol Chem 193:265–275

    CAS  PubMed  Google Scholar 

  • Martinez D, Berka RM, Henrissat B, Saloheimo M, Arvas M, Baker SE, Chapman J, Chertkov O, Coutinho PM, Cullen D, Danchin EGJ, Grigoriev IV, Harris P, Jackson M, Kubicek CP, Han CS, Ho I, Larrondo LF, De Leon AL, Magnuson JK, Merino S, Misra M, Nelson B, Putnam N, Robbertse B, Salamov AA, Schmoll M, Terry A, Thayer N, Westerholm-Parvinen A, Schoch CL, Yao J, Barbote R, Nelson MA, Detter C, Bruce D, Kuske CR, Xie G, Richardson P, Rokhsar DS, Lucas SM, Rubin EM, Dunn-Coleman N, Ward M, Brettin TS (2008) Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina). Nat Biotechnol 26:553–560

    Article  CAS  PubMed  Google Scholar 

  • Marx I, van Wyk N, Smit S, Jacobson D, Viljoen-Bloom M, Volschenk H (2013) Comparative secretome analysis of Trichoderma asperellum S4F8 and Trichoderma reesei Rut C30 during solid-state fermentation on sugarcane bagasse. Biotechnol Biofuels 6:172–185

    Article  PubMed Central  PubMed  Google Scholar 

  • Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428

    Article  CAS  Google Scholar 

  • Miller I, Crawford J, Gianazza E (2006) Protein stains for proteomic applications: Which, when, why? Proteomics 6:5385–5408

    Article  CAS  PubMed  Google Scholar 

  • Oda K, Kakizono D, Yamada O, Iefuji H, Akita O, Iwashita K (2006) Proteomic analysis of extracellular proteins from Aspergillus oryzae grown under submerged and solid-state culture conditions. Appl Environ Microbiol 72:3448–3457

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Pablo A, Ballesteros M, Negro MJ (2013) Progress on enzymatic saccharification technologies for biofuels production. In: Gupta VK, Tuohy MG (eds)  Biofuel technologies: Recent Developments. Springer, Berlin, pp 145–169

  • Ravalason H, Grisel S, Chevret D, Favel A, Berrin JG, Sigoillot JC, Herpoël-Gimbert I (2012) Fusarium verticillioides secretome as a source of auxiliary enzymes to enhance saccharification of wheat straw. Bioresour Technol 114:589–596

    Article  CAS  PubMed  Google Scholar 

  • Resing KA, Ahn NG (2005) Proteomics strategies for protein identification. FEBS Lett 579:885–889

    Article  CAS  PubMed  Google Scholar 

  • Rezende MI, Barbosa ADM, Flora A, Vasconcelos D, Endo AS (2002) Xylanase production by Trichoderma harzianum Rifai by solid state fermentation on sugarcane bagasse. Braz J Microbiol 33:67–72

    Article  CAS  Google Scholar 

  • Sandgren M, Stahlberg J, Mitchinson C (2005) Structural and biochemical studies of GH family 12 cellulases: improved thermal stability, and ligand complexes. Prog Biophys Mol Biol 89:246–291

    Article  CAS  PubMed  Google Scholar 

  • Scheller HV, Ulvskov P (2010) Hemicelluloses. Ann Rev Plant Biol 61:263–289

    Article  CAS  Google Scholar 

  • Shallom D, Shoham Y (2003) Microbial hemicellulases. Curr Opin Microbiol 6:219–223

    Article  CAS  PubMed  Google Scholar 

  • Stricker AR, Mach RL, de Graaff LH (2008) Regulation of transcription of cellulase- and hemicellulase-encoding genes in Aspergillus niger and Hypocrea jecorina (Trichoderma reesei). Appl Microbiol Biotechnol 78:211–220

    Article  CAS  PubMed  Google Scholar 

  • Sukumaran RK, Singhania RR, Mathew GM, Pandey A (2009) Cellulase production using biomass feed stock and its application in lignocellulose saccharification for bio-ethanol production. Renew Energy 34:421–424

    Article  CAS  Google Scholar 

  • Van Burik J, Schreckhise RW, White TC, Bowden RA, Myerson D (1998) Comparison of six extraction techniques for isolation of DNA from filamentous fungi. Med Mycol 36:299–303

    Article  PubMed  Google Scholar 

  • Wilkins MR, Williams KL (1997) Cross-species protein identification using amino acid composition, peptide mass fingerprinting, isoelectric point and molecular mass: a theoretical evaluation. J Theor Biol 186:7–15

    Article  CAS  PubMed  Google Scholar 

  • Wilm M, Shevchenko A, Houthaeve T, Breit S, Schweigerer L, Fotsis T, Mann M (1996) Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry. Nature 379:466–469

    Article  CAS  PubMed  Google Scholar 

  • Xie ZL, Gao HY, Zhang Q, Wang H, Liu Y (2012) Cloning of a novel xylanase gene from a newly isolated Fusarium sp. Q7-31 and its expression in Escherichia coli. Braz J Microbiol 43(1):405–417

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Yan JX, Wait R, Berkelman T, Harry RA, Westbrook JA, Wheeler CH, Dunn MJ (2000) A modified silver staining protocol for visualization of proteins compatible with matrix-assisted laser desorption/ionization and electrospray ionization-mass spectrometry. Electrophoresis 21:3666–3672

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We gratefully acknowledge the financial support from the National Science Foundation of China (NSFC, project No.31260021) and the Science and Technology Office of Qinghai province (project No.2014-ZJ-903).

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Authors and Affiliations

  1. College of Ecological and Environment Engineering, State Key Laboratory Breeding Base for innovation and utilization of plateau crop germplasm, University of Qinghai, 251 Ningda Road, Xining, Qinghai, 810016, People’s Republic of China

    Fei Tian, Zhan-ling Xie, Lian-zheng Zhao, Jing Guo, Xing-bao Han, Li-fang Xie, Yan Wang & Xin-yuan Chang

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  1. Fei Tian
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  2. Zhan-ling Xie
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  3. Lian-zheng Zhao
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  6. Li-fang Xie
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  7. Yan Wang
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Correspondence to Zhan-ling Xie.

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Tian, F., Xie, Zl., Zhao, Lz. et al. Comparative secretome analysis of Fusarium sp. Q7-31T during liquid fermentation using oat straw as a carbon source. Ann Microbiol 65, 2131–2140 (2015). https://doi.org/10.1007/s13213-015-1051-z

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  • DOI: https://doi.org/10.1007/s13213-015-1051-z

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