Abstract
We describe here a simple technological process based on the direct fermentation of potato starch waste (PSW), an inexpensive agro-processing industrial waste, by a potential probiotic strain, Lactococcus lactis subsp. lactis, for enhancing L-lactic acid production. To maximize bioconversion and increase cell stability, we designed and tested a novel dialysis sac-based bioreactor. Shake flask fermentation (SFF) and fed batch fermentation in the dialysis sac bioreactor were compared for L-lactic acid production efficiency. The results showed that the starch (20 g/L) in the PSW-containing medium was completely consumed within 24 h in the dialysis sac bioreactor, compared with 48 h in the SFF. The maximum lactic acid concentration (18.9 g/L) and lactic acid productivity (0.79 g/L·h) obtained was 1.2- and 2.4-fold higher in the bioreactor than by SFF, respectively. Simultaneous saccharification and fermentation was effected at pH 5.5 and 30 °C. L. lactis cells were viable for up to four cycles in the fed batch fermentation compared to only one cycle in the SFF.
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Acknowledgments
The authors thank the University Grants Commission for financial support in the form of a fellowship (RGNF) to one of the authors (Ms. Seema Bhanwar) and the Director, Thapar University, for providing the infrastructure for the work.
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Bhanwar, S., Singh, A. & Ganguli, A. Effective conversion of industrial starch waste to L-Lactic acid by Lactococcus lactis in a dialysis sac bioreactor. Ann Microbiol 64, 1447–1452 (2014). https://doi.org/10.1007/s13213-013-0754-2
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DOI: https://doi.org/10.1007/s13213-013-0754-2