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Aflatoxigenic strains of Aspergillus section Flavi isolated from marketed peanuts (Arachis hypogaea) in Algiers (Algeria)

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Abstract

The present study reports on the natural mycobiota occurring in Chinese peanuts marketed in Algiers, paying special attention to the incidence of Aspergillus section Flavi species that are potential producers of aflatoxins. The mean value counts of fungi ranged from 155 to 577 CFU/g dry matter (DM) and the predominant fungi were different species of the genus Penicillium (83.81–93.85 %) and Aspergillus belonging to section Flavi (2.73–73.96 %). Results indicated that 82 isolates (100 %) were aflatoxigenic. The Aspergillus section Flavi strains revealed that 65 isolates (79.27 %) were highly aflatoxigenic, producing four kinds of aflatoxins [AFB1 (0.846–3.330 μg/g), AFB2 (0.005–0.007 μg/g), AFG1 (0.008–1.595 μg/g), and AFG2 (0.005–0.010 μg/g)], whereas 17 isolates (20.73 %) synthetized low levels of one or two aflatoxins (AFB1 and AFG2). Aflatoxin production was also screened on Coconut Agar Medium (CAM), and the results were consistent with the HPLC analysis. Based on the combination of mycotoxins produced, five Aspergillus section Flavi chemotypes were established. Sclerotia production expressed a correlation to aflatoxigenicity. The total aflatoxins were detected in four analyzed samples at levels ranging from 0.71 to 25.50 μg/kg. Furthermore, the amplicons corresponding to the ITS1-5.8 S-ITS2 rDNA of six representative strains showed that four strains belonged to Aspergillus flavus, one to A. minisclerotigenes, and one to A. caelatus. The results obtained indicate that there is a possible risk factor posed by aflatoxins contamination of peanuts marketed in Algiers.

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References

  • Abbas HK, Weaver MA, Zablotowicz RM, Horn BW, Shier WT (2005) Relationships between aflatoxin production and sclerotia formation among isolates of Aspergillus Section Flavi from the Mississippi Delta. Eur J Plant Pathol 112:283–287

    Article  Google Scholar 

  • Barros G, Torres A, Palacio G, Chulze S (2003) Aspergillus species from section Flavi isolated from soil at planting and harvest time in peanut-growing regions of Argentina. J Sci Food Agric 83:1303–1307

    Article  CAS  Google Scholar 

  • Bayman P, Cotty PJ (1993) Genetic diversity in Aspergillus flavus: association with aflatoxin production and morphology. Can J Bot 71:23–34

    Article  CAS  Google Scholar 

  • Bennett JW (1987) Mycotoxins, mycotoxicoses, mycotoxicology. Mycopathologia 100:3–5

    Article  PubMed  CAS  Google Scholar 

  • Bennett JW, Klich M (2003) Mycotoxins. Clin Microbiol Rev 16:497–516

    Article  PubMed  CAS  Google Scholar 

  • Berthier J, Valla G (1998) Moisissures - mycotoxines et aliments: du risque à la prévention. Rev Med Vet 149:479–492

    Google Scholar 

  • Blankenship PD, Cole RJ, Sanders TH, Hill RA (1984) Effect of geocarposphere temperature on preharvest colonization of drought stressed peanut by Aspergillus flavus and subsequent aflatoxin contamination. Mycopathologia 85:69–74

    Article  PubMed  CAS  Google Scholar 

  • Bourais I, Amine A (2006) Aflatoxines toxiques redoutables dans nos aliments. Les techniques de laboratoire. No. 0/2006. p. 4–8

  • Bragulat MR, Abarca ML, Cabanes FJ (2001) An easy screening method for fungi producing ochratoxin A in pure culture. Int J Food Microbiol 71:139–144

    Article  PubMed  CAS  Google Scholar 

  • Cahagnier B (1998) Moisissures des aliments peu hydratés. Lavoisier Tec & Doc, Paris

    Google Scholar 

  • CAMAG (1997). Determination of aflatoxins B1, B2, G1 and G2 in foodstuffs. CAMAG, Application notes, BIBLIOGRAPHY SERVICE (CBS). Thin-layer chromatography. 79, p. 10

  • Castegnaro M, Wild CP (1995) IARC activities in mycotoxin research. In Nat Toxins 3:327–331

    Article  CAS  Google Scholar 

  • Chang PK, Bennett JW, Cotty PJ (2001) Association of aflatoxin biosynthesis and sclerotial development in Aspergillus parasiticus. Mycopathologia 153:41–48

    Article  Google Scholar 

  • Cole G, Kendrick B (1981) Biology of conidial fungi. Academic, London

    Google Scholar 

  • Cotty PJ (1989) Virulence and cultural characteristics of two Aspergillus flavus strains pathogenic on cotton. Phytopathology 79:808–814

    Article  Google Scholar 

  • Cotty PJ (1997) Aflatoxin-producing potential of communities of Aspergillus section Flavi from cotton producing areas in the United States. Mycol Res 101:698–704

    Article  Google Scholar 

  • Davis ND, Iyer SK, Diener UL (1987) Improved method of screening for aflatoxin with a coconut agar medium. Appl Environ Microbiol 53:1593–1595

    PubMed  CAS  Google Scholar 

  • Dehghan P, Zaini F, Mahmoudi M, Jebali A, Kordbacheh P, Rezaei S (2008) Aflatoxin and sclerotia production in clinical isolates of Aspergillus flavus group. Iranian J Publ Health 37:41–50

    Google Scholar 

  • D’Mello JPF, McDonald AMC (1997) Mycotoxins. Anim Feed Sci Technol 69:155–166

    Article  Google Scholar 

  • Dorner JW, Cole RJ (2002) Effect of application of nontoxigenic strain of Aspergillus flavus and A. parasiticus on subsequent aflatoxin contamination of peanut in storage. J Stored Prod Res 38:329–339

    Article  CAS  Google Scholar 

  • Egel DS, Cotty PJ, Ellias KS (1994) Relationships among isolates of Aspergillus sect. Flavi that vary in aflatoxin production. Phytopathology 84:906–912

    Article  CAS  Google Scholar 

  • Ellis WO, Smith PJ, Simpson BK, Khanizadeh S, Oldham JH (1993) Control of growth and aflatoxin production of Aspergillus flavus under modified atmosphere packaging conditions. Food Microbiol 10:9–21

    Article  CAS  Google Scholar 

  • European Commission (2006) Final report of a mission carried out in China, in order to assess the felicities and measures in place for the control of aflatoxin contamination in peanuts intended for export into the UE. Report DG (SANCO)/8126/2006

  • European Union (UE) (2005) Commission Regulation (EC) N°. 856/2005. Official Journal of the European Union 48 (6 June 2005), 3–8

  • Felsentein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    Article  Google Scholar 

  • Fente CA, Ordaz JJ, Vazquez BI, Franco CM, Cepeda A (2001) New additive for culture media for rapid identification of aflatoxin-producing Aspergillus strains. Appl Environ Microbiol 67:4858–4862

    Article  PubMed  CAS  Google Scholar 

  • Frisvad JC, Samson RA, Smedsgaard J (2004) Emericella astellata, a new producer of aflatoxin B1, B2 and sterigmatocystin. Lett Appl Microbiol 38:440–445

    Article  PubMed  CAS  Google Scholar 

  • Gabal MA, Hegazi SA, Hassanin N (1994) Aflatoxin production by Aspergillus flavus field isolates. Vet Hum Toxicol 36:519–521

    PubMed  CAS  Google Scholar 

  • Geiser DM, Dorner JW, Horn BW, Taylor JW (2000) The phylogenetics of mycotoxin and sclerotium production in Aspergillus flavus and Aspergillus oryzea. Fungal Genet Biol 31:169–179

    Article  PubMed  CAS  Google Scholar 

  • Giorni P, Magan N, Pietri A, Bertuzzi T, Battilani P (2007) Studies of Aspergillus section Flavi isolated from maize in northen Italy. Int J Food Microbiol 113:330–338

    Article  PubMed  CAS  Google Scholar 

  • Goncalez E, Nogueira JH, Fonseca H, Felicio JD, Pino FA, Correa B (2008) Mycobiota and mycotoxins in Brazilian peanut kernels from sowing to harvest. Int J Food Microbiol 123:184–190

    Article  PubMed  CAS  Google Scholar 

  • Hanlin RT (1969) Fungi in developing peanut fruits. Mycopath Mycol Appl 38:93–100

    Article  Google Scholar 

  • Hill RA, Lacey J (1983) Factors determining the microflora of stored barley grain. Ann App Biol 102:467–483

    Article  Google Scholar 

  • Horn BW (2006) Relationship between soil densities of Aspergillus species and colonization of wounded peanut seeds. Can J Microbiol 52:951–960

    Article  PubMed  CAS  Google Scholar 

  • Horn BW, Dorner JW (1998) Soil populations of Aspergillus species from section Flavi along a transect through peanut-growing regions of the United States. Mycologia 90:767–776

    Article  Google Scholar 

  • Horn BW, Greene RL, Dorner JW (1995) Effect of corn and peanut cultivation on soil populations of Aspergillus flavus and A. parasiticus in southwestern Georgia. Appl Environ Microbiol 61:2472–2475

    PubMed  CAS  Google Scholar 

  • Horn BW, Greene RL, Sorensen RB, Blankenship PD, Dorner JW (2001) Conidial movement of nontoxigenic Aspergillus flavus and Aspergillus parasiticus in peanut fields following application to soil. Mycopathologia 151:81–92

    Article  PubMed  CAS  Google Scholar 

  • IARC (1982) The evaluation of the carcinogenic risk of chemical to humans, IARC Monograph Supplement, vol 4, 4th edn. International Agency for Research on Cancer, Lyon

    Google Scholar 

  • Jukes TH, Cantor CR (1969) Evolution of Protein Molecules. In: Murno HN (ed). Academic, New York, pp 21–132

  • Klich MA (1998) Soil fungi of some low-altitude desert cotton fields and ability of their extracts to inhibit Aspergillus flavus. Mycopathologia 142:97–100

    Article  PubMed  CAS  Google Scholar 

  • Klich MA (2007) Environmental and developmental factors influencing aflatoxin production by Aspergillus flavus and Aspergillus parasiticus. Mycoscience 48:71–80

    Article  CAS  Google Scholar 

  • Kozakiewicz Z (1989) Aspergillus species on stored products. Mycological Papers, vol 161. CAB International, Wallingford

    Google Scholar 

  • Liu D, Coloe S, Baird R, Pedersen J (2000) Rapid Mini-Preparation of Fungal DNA for PCR. J Clin Microbiol 38:471

    PubMed  CAS  Google Scholar 

  • Luchese RH, Harrigan WF (1993) Biosynthesis of aflatoxin – the role of nutritional factors. J Appl Bacteriol 74:5–14

    Article  PubMed  CAS  Google Scholar 

  • Mishra HN, Das C (2003) A review on biological control and metabolism of aflatoxin. Crit Rev Food Sci Nutr 43:245–264

    Article  PubMed  CAS  Google Scholar 

  • Molina M, Gianuzzi L (2002) Modelling of aflatoxin production by Aspergillus parasiticus in a solid medium at different temperatures, pH and propionic acid concentrations. Food Res Int 35:585–594

    Article  CAS  Google Scholar 

  • Moss MO (1996) Mode of formation of ochratoxin A. Food Addit Contam 135:5–9

    Google Scholar 

  • Nicholson P, Chandler E, Simpson DR, Thomsett M, WILSON A (2003) Molecular methods for species and chemotype detection of toxigenic fungi. Asp App Bio 68:11–20

    Google Scholar 

  • Novas MV, Cabral D (2002) Association of mycotoxin and sclerotia production with compatibility groups in Aspergillus flavus from peanut in Argentina. Plant Dis 86:215–219

    Article  CAS  Google Scholar 

  • Okano K, Tomita T, Kumeda Y, Matsumaru K, Ichinoe M (2008) Aflatoxin B and G contamination in imported peanuts [Arachis hypogaea] and Aspergillus section Flavi of the causing fungi. Mycotoxins. AGRIS Categories. Food Contamin Toxicol 58:107–114

    CAS  Google Scholar 

  • Payne GA, Brown MP (1998) Genetics and Physiology of aflatoxin biosynthesis. Annu Rev Phytopath 36:329–362

    Article  CAS  Google Scholar 

  • Pildain MB, Vaamonde G, Cabral D (2004) Analysis of population structure of Aspergillus flavus from peanut based on vegetative compatibility, geographic origin, mycotoxin and sclerotia production. Int J Food Microbiol 93:31–40

    Article  PubMed  CAS  Google Scholar 

  • Pildain MB, Frisvad JC, Vaamonde G, Cabral D, Varga J, Samson RA (2008) Two novel aflatoxin producing Aspergillus species from Argentinean peanuts. Int J Sysyt Evol Microbiol 58:725–735

    Article  CAS  Google Scholar 

  • Pitt JI, Hocking AD (1997) Fungi and food Spoilage, 2nd edn. Blackie, London

    Book  Google Scholar 

  • Pitt JI, Hocking AD, Bhudhasamai K, Miscamble BF, Wheeler KA, Tanboon-Ek P (1993) The normal mycoflora of commodities from Thailand. 1. Nuts and oilseeds. Int J Food Microbiol 20:211–226

    Article  PubMed  CAS  Google Scholar 

  • Raper KB, Fennell DI (1977) The genus Aspergillus. Krieger, Malabar

    Google Scholar 

  • Razzaghi-Abyaneh M, Shams-Ghahf Arokhi M, Allameh A, Kazeroon-Shiri A, Ranjbar-Bahadori S, Mirzahoseini H, Rezaee MB (2006) A survey on distribution of Aspergillus section Flavi in corn field soils in Iran: Population patterns based on aflatoxins, cyclopiazonic acid and sclerotia production. Mycopathologia 161:183–192

    Article  PubMed  CAS  Google Scholar 

  • Reboux G (2006) Mycotoxines: effets sur la santé et interactions avec d’autres composants organiques. Rev Fr Allergol 46:208–212

    Article  Google Scholar 

  • Riba A, Bouras N, Mokrane S, Mathieu F, Lebrihi A, Sabaou N (2010) Aspergillus section Flavi and aflatoxins in Algerian wheat and derived products. Food Chem Toxicol 48:2772–2777

    Article  PubMed  CAS  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  • Sultan Y, Magan N (2010) Mycotoxigenic fungi in peanuts from different geographic regions of Egypt. Mycotoxin Res 26:133–140

    Article  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  PubMed  CAS  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–80

    Article  PubMed  CAS  Google Scholar 

  • Turner NW, Subrahmanyam S, Piletsky SA (2009) Analytical methods for determination of mycotoxins: a review. Anal Chim Acta 632:168–180

    Article  PubMed  CAS  Google Scholar 

  • Vaamonde G, Patriarca A, Fernandez Pinto V, Comerio R, Degrossi C (2003) Variability of aflatoxin and cyclopiazonic acid production by Aspergillus section Flavi from different substrates in Argentina. Int J Food Microbiol 88:79–84

    Article  PubMed  CAS  Google Scholar 

  • Varga J, Frisvad JC, Samson RA (2009) A reappraisal of fungi producing aflatoxins. World Mycotoxin J 2:263–277

    Article  CAS  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR Protocols: a guide to methods and Applications. Academic, San Diego, pp 315–322

    Google Scholar 

  • Williams JH, Phillips TD, Jolly PE, Stiles JK, Jolly CM, Aggarwal D (2004) Human aflatoxicosis in developing countries: a review of toxicology, exposure, potential health consequences, and interventions. Am J Clin Nutr 80:1106–1122

    PubMed  CAS  Google Scholar 

  • Yu J, Bathnagar D, Cleveland TE (2004) Completed sequence of aflatoxin pathway gene cluster in Aspergillus parasiticus. FEBS Lett 564:126–130

    Article  PubMed  CAS  Google Scholar 

  • Zehrer W (1996) Promotion de la Protection Intégrée des Cultures et des Denrées Stockées à Madagascar. Recueil des exposés lors du Symposium tenu à Toliara du 7 au 11 Octobre 1996. Service de la Surveillance Phytosanitaire du Territoire National Direction de la Protection des Végétaux. BP 1042 – Nanisana. 101 Antananarivo. Madagascar

  • Zhang Z, Schwartz S, Wagner L, Miller W (2000) A greedy algorithm for aligning DNA sequences. J Comput Biol 7:203–14

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Microbiologie. Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumediene, BP. 32 El Alia 16111, Bab Ezzouar, Algiers, Algeria

    Nadjet Guezlane-Tebibel

  2. Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, B.P. 92, 16 050, Vieux-Kouba, Algiers, Algeria

    Noureddine Bouras & Salim Mokrane

  3. Laboratoire Central de Police Scientifique, Département de Sécurité Alimentaire et Environnement, 1 rue Abdelaziz Khelalfa, Châteauneuf, Ben Aknoun, Algiers, Algeria

    Tahar Benayad

  4. Université de Toulouse, Laboratoire de Génie Chimique UMR 5503 (CNRS/INPT/UPS), ENSAT/INP de Toulouse, 1 avenue de l’Agrobiopôle, Castanet-Tolosan Cedex, France

    Florence Mathieu

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  1. Nadjet Guezlane-Tebibel
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Guezlane-Tebibel, N., Bouras, N., Mokrane, S. et al. Aflatoxigenic strains of Aspergillus section Flavi isolated from marketed peanuts (Arachis hypogaea) in Algiers (Algeria). Ann Microbiol 63, 295–305 (2013). https://doi.org/10.1007/s13213-012-0473-0

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