Skip to main content
SpringerLink
Log in

Phytase production by Bacillus subtilis US417 in submerged and solid state fermentations

  • Original Article
  • Published:
Annals of Microbiology Aims and scope Submit manuscript

Abstract

When the variables (inoculum size, methanol and yeast extract) identified to affect phytase production by Bacillus subtilis US417 using Plackett-Burman design were optimized by RSM, a high enzyme production of 112 U/g of wheat bran was attained. Overall, a 5-fold improvement in phytase production was achieved. In SSF, on the other hand, a 4-fold enhancement in enzyme titer was attained (85 U/g of wheat bran). Based on these findings, phytase productivity was higher in SF [2.3 U/(g × h)] than in SSF [1.2 U/(g × h)].

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Choi YM, Dong ON, Cho SH, Lee HK, Suh HJ, Chung SH (1999) Isolation of a phytase producing Bacillus sp. KHU-10 and its phytase production. J Microbiol Biotechnol 9(2):223–236

    CAS  Google Scholar 

  • Dijkhuizen L, Levering PR (1992) De Vries GE. In: Murrell JC, Dalton H (eds) Methane and methanol utilizers. Plenum, New York, p 149

    Google Scholar 

  • Ebune A, Al-Asheh S, Duvnjak Z (1995) Production of phytase during solid-state fermentation using Aspergillus ficuum NRRL 3135 in canola meal. Bioresour Technol 53:7–12

    Article  CAS  Google Scholar 

  • Engelen AJ, van der Heeft FC, Randsdorp PHG, Smit ELC (1994) Simple and rapid determination of phytase activity, J Assoc Off Anal Chem 77:760–764

    Google Scholar 

  • Farhat A, Chouayekh H, Ben Farhat M, Bouchaala K, Bejar S (2008) Gene cloning and characterization of a thermostable phytase from Bacillus subtilis US417 and assessment of its potential as a feed additive in comparison with a commercial enzyme. Mol Biotechnol 40(2):127–135

    Article  PubMed  CAS  Google Scholar 

  • Greiner R, Egli I (2003) Determination of the activity of acidic phytate-degrading enzymes in cereal seeds. J Agric Food Chem 51:847–850

    Article  PubMed  CAS  Google Scholar 

  • Greiner R, Konietzny U (2006) Phytase for food application. Food Technol Biotechnol 44(2):125–140

    CAS  Google Scholar 

  • Gulati HK, Chadha BS, Saini HS (2007) Production and characterization of thermostable alkaline phytase from Bacillus laevolacticus isolated from rhizosphere soil. J Ind Microbiol Biotechnol 34:91–98

    Article  PubMed  CAS  Google Scholar 

  • Gunashree BS, Venkateswaran G (2008) Effect of different cultural conditions for phytase production by Aspergillus niger CFR 335 in submerged and solid-state fermentations. J Ind Microbiol Biotechnol 35(12):1587–1596

    Article  PubMed  CAS  Google Scholar 

  • Haefner S, Knietsch A, Scholten E, Braun J, Lohscheidt M, Zelder O (2005) Biotechnological production and applications of phytases. Appl Microbiol Biotechnol 68(5):588–597

    Article  PubMed  CAS  Google Scholar 

  • Heck JX, Flôres SH, Hertz PF, Ayub MAZ (2006) Statistical optimization of thermo-tolerant xylanase activity from Amazon isolated Bacillus circulans on solid-state cultivation. Bioresour Technol 97:1902–1906

    Article  PubMed  CAS  Google Scholar 

  • Jareonkitmongkol S, Ohya M, Watanabe R, Takagi H, Nakamori S (1997) Partial purification of phytase from a soil isolate bacterium, Klebsiella oxytoca MO-3. J Ferment Bioeng 83(4):393–394

    Article  CAS  Google Scholar 

  • Kammoun R, Chouayekh H, Abid H, Naili B, Bejar S (2009) Purification of CBS 819.72 α-amylase by aqueous two-phase systems: modelling using response surface methodology. Biochem Eng J 46:306–312

    Article  CAS  Google Scholar 

  • Kerovuo J, Tynkkynen S (2000) Expression of Bacillus subtilis phytase in Lactobacillus plantarum 755. Lett Appl Microbiol 30(4):325–329

    Article  PubMed  CAS  Google Scholar 

  • Kim YO, Kim HK, Bae KS, Yu JH, Oh TK (1998) Purification and properties of thermostable phytase from Bacillus sp. DS11. Enzyme Microb Technol 22:2–7

    Article  CAS  Google Scholar 

  • Kim DS, Godber JS, Kim HR (1999) Culture conditions for a new phytase-producing fungus. Biotechnol Lett 21:1077–1181

    Article  CAS  Google Scholar 

  • Krishna C, Nokes SE (2001) Predicting vegetative inoculum performance to maximize phytase production in solid-state fermentation using response surface methodology. J Ind Microbiol Biotechnol 26:161–170

    Article  PubMed  CAS  Google Scholar 

  • Lan GQ, Abdullah N, Jalaludin S, Ho YW (2002) Culture conditions influencing phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle. J Appl Microbiol 93(4):668–674

    Article  PubMed  CAS  Google Scholar 

  • Nampoothiri KM, Tomes GJ, Krishnan R, Szakacs G, Nagy V, Soccol CR, Pandey A (2004) Thermostable phytase production by Thermoascus aurantiacus in submerged fermentation. Appl Biochem Biotechnol 118(1–3):205–214

    Article  PubMed  CAS  Google Scholar 

  • Ramachandran S, Roopesh K, Nampoothiri KM, Szakacs G, Pandey A (2005) Mixed substrate fermentation for the production of phytase by Rhizopus sp. using oilcakes as substrates. Process Biochem 40:1749–1754

    Article  CAS  Google Scholar 

  • Rao DECS, Rao KV, Reddy VD (2008) Cloning and expression of Bacillus phytase gene (phy) in ‘Escherichia coli’ and recovery of active enzyme from the inclusion bodies. J Appl Microbiol 105(4):1128–1137

    Article  PubMed  CAS  Google Scholar 

  • Roopesh K, Ramachandran SI, Nampoothiri KM, Szakacs G, Pandey A (2006) Comparison of phytase production on wheat bran and oilcakes in solid-state fermentation by Mucor racemosus. Bioresour Technol 97:506–511

    Article  PubMed  CAS  Google Scholar 

  • Servi S, Zkaya HO, Colakoglu AS (2008) Dephytinization of wheat bran by fermentation with bakers’ yeast, incubation with barley malt flour and autoclaving at different pH levels. J Cereal Sci 48:471–476

    Article  CAS  Google Scholar 

  • Singh B, Satyanarayana T (2008) Improved phytase production by a thermophilic mould Sporotrichum thermophile in submerged fermentation due to statistical optimization. Bioresour Technol 99:824–830

    Article  PubMed  CAS  Google Scholar 

  • Sung HG, Shin HT, Ha JK, Lai HL, Cheng KJ, Lee JH (2005) Effect of germination temperature on characteristics of phytase production from barley. Bioresour Technol 96:1297–1303

    Article  PubMed  CAS  Google Scholar 

  • Sunitha K, Lee JK, Oh TK (1999) Optimization of medium components for phytase production by E. coli using response surface methodology. Bioprocess Eng 21:477–481

    CAS  Google Scholar 

  • Vats P, Banerjee UC (2004) Production studies and catalytic properties of phytases (myo-inositolhexakisphosphate phosphohydrolases): an overview. Enzyme Microb Technol 35:3–14

    Article  CAS  Google Scholar 

  • Vuolanto A, Weymarn NV, Kerovuo J, Ojamo H, Leisola M (2001) Phytase production by high cell density culture of recombinant Bacillus subtilis. Biotechnol Lett 23:761–766

    Article  CAS  Google Scholar 

  • Xiong AS, Yao QH, Peng RH, Han PL, Cheng ZM, Li Y (2005a) High level expression of a recombinant acid phytase gene in Pichia pastoris. J Appl Microbiol 98:418–428

    Article  PubMed  CAS  Google Scholar 

  • Xiong C, Shouwen C, Ming S, Ziniu Y (2005b) Medium optimization by response surface methodology for poly-γ-glutamic acid production using dairy manure as the basis of a solid substrate. Appl Microbiol Biotechnol 69(4):390–396

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This work was funded by the Tunisian Government “Contrat Programme CBS-LMB” and the local Company “Nutrisud” through a research agreement. The authors wish to express their gratitude to Mr. Moncef Affes for his critical reading of the manuscript. Thanks are also due to Mr Anouar Smaoui from the Sfax Faculty of Sciences for carefully proofreading and editing the present paper.

Author information

Authors and Affiliations

  1. Laboratoire de Microorganismes et Biomolécules, Centre de Biotechnologie de Sfax, Université de Sfax, Route de Sidi Mansour Km 6, BP “1177” 3018, Sfax, Tunisie

    Radhouane Kammoun, Ameny Farhat, Hichem Chouayekh, Kameleddine Bouchaala & Samir Bejar

Authors
  1. Radhouane Kammoun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ameny Farhat
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hichem Chouayekh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kameleddine Bouchaala
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Samir Bejar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Radhouane Kammoun.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kammoun, R., Farhat, A., Chouayekh, H. et al. Phytase production by Bacillus subtilis US417 in submerged and solid state fermentations. Ann Microbiol 62, 155–164 (2012). https://doi.org/10.1007/s13213-011-0240-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13213-011-0240-7

Keywords

Search

Navigation