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Solid-state protease production using anchovy waste meal by moderate halophile Serratia proteamaculans AP-CMST isolated from fish intestine

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Abstract

The possibility of utilising anchovy waste meal as a substrate for protease production by the fish gut isolate Serratia proteamaculans AP-CMST was assessed through solid-state fermentation. A time course for protease production revealed 72 h to be the optimum duration for higher production (146.24 U/g). The most suitable pH, temperature and moisture level observed for higher protease production were pH 7 (123.5 U/g), 30°C (97.22 U/g) and 75% (126.7 U/g), respectively. Protease production by S. proteamaculans AP-CMST was high in medium with added xylose (198.21 U/g), peptone (118.42 U/g), Triton X-100 (152.56 U/g) and manganese sulphate (178.33 U/g) when compared to other tested medium components. The halotolerancy of S. proteamaculans AP-CMST for protease production was 4% sodium chloride (155.65 U/g). Enzyme recovery from fermented anchovy waste meal was greatest (130.52 U/g) when 10% ethyl acetate was used as the extractant, and the optimum time range for extraction was 90–120 min.

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Acknowledgements

We gratefully acknowledge the financial assistance extended in the form of Senior Research Fellowship by the Council of Scientific and Industrial Research (CSIR), Ministry of Human Resource Development, Government of India to P.E. Also we thank Dr. Georgina Sandoval (Industrial Biotechnology Department, CIATEJ, Guadalajara, Mexico) for her valuable suggestions, support and constant encouragement.

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Authors and Affiliations

  1. Marine Biotechnology Laboratory, Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, 629 502, Kanyakumari District, Tamilnadu, India

    Palanichamy Esakkiraj, Suppiah Sankaralingam, Raj Usha, Arunachalam Palavesam & Grasian Immanuel

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  1. Palanichamy Esakkiraj
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  2. Suppiah Sankaralingam
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  3. Raj Usha
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  4. Arunachalam Palavesam
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Correspondence to Arunachalam Palavesam.

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Esakkiraj, P., Sankaralingam, S., Usha, R. et al. Solid-state protease production using anchovy waste meal by moderate halophile Serratia proteamaculans AP-CMST isolated from fish intestine. Ann Microbiol 61, 749–755 (2011). https://doi.org/10.1007/s13213-010-0191-4

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