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Evaluation of thermotolerant and ethanol-tolerant Saccharomyces cerevisiae as an alternative strain for bioethanol production from industrial feedstocks

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Abstract

Despite the fact that yeast Saccharomyces cerevisiae is by far the most commonly used in ethanol fermentation, few have been reported to be resistant to high ethanol concentrations at high temperatures. Hence, in this study, 150 S. cerevisiae strains from the Thailand Bioresource Research Center (TBRC) were screened for ethanol production based on their glucose utilization capability at high temperatures. Four strains, TBRC 12149, 12150, 12151, and 12153, exhibited the most outstanding ethanol production at high temperatures in shaking-flask culture. Among these, strain TBRC 12151 demonstrated a high ethanol tolerance of up to 12% at 40 °C. Compared to industrial and laboratory strains, TBRC 12149 displayed strong sucrose fermentation capacity whereas TBRC 12153 and 12151, respectively, showed the greatest ethanol production from molasses and cassava starch hydrolysate at high temperatures in shaking-flask conditions. In 5-L batch fermentation, similarly to both industrial strains, strain TBRC 12153 yielded an ethanol concentration of 66.5 g L−1 (58.4% theoretical yield) from molasses after 72 h at 40 °C. In contrast, strain TBRC12151 outperformed other industrial strains in cell growth and ethanol production from cassava starch hydrolysis at 40 °C with an ethanol production of 65 g L−1 (77.7% theoretical yield) after 72 h. Thus, the thermotolerant and ethanol-tolerant S. cerevisiae TBRC 12151 displayed great potential and possible uses as an alternative strain for industrial ethanol fermentation using cassava starch hydrolysate.

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All data generated or analysed during this study are included in this article and its supplementary information files.

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Acknowledgements

WK is thankful to the Postdoctoral Research Fellowship from National Center for Genetic Engineering and Biotechnology (BIOTEC). We are grateful to Dr. Philip J. Shaw for critically editing the manuscript.

Funding

This work was supported by BIOTEC (Grant numbers: P-18-52414 and P-19-52258).

Author information

Author notes

  1. Worarat Kruasuwan

    Present address: Siriraj Long-Read Laboratory (Si-LoL), Division of Medical Bioinformatics, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand

Authors and Affiliations

  1. Microbial Cell Factory Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Khlong Nueng, Khlong Luang, 12120, Pathum Thani, Thailand

    Worarat Kruasuwan, Aekkachai Puseenam, Kanokarn Kocharin, Sutipa Tanapongpipat & Niran Roongsawang

  2. Microbial Diversity and Utilization Research Team, Thailand Bioresource Research Center, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Khlong Nueng, Khlong Luang, 12120, Pathum Thani, Thailand

    Somjit Am-in & Sasitorn Jindamorakot

  3. Enzyme Technology Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Khlong Nueng, Khlong Luang, 12120, Pathum Thani, Thailand

    Srisakul Trakarnpaiboon & Warasirin Sornlek

  4. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China

    Feng-Yan Bai

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  1. Worarat Kruasuwan
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Correspondence to Niran Roongsawang.

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Kruasuwan, W., Puseenam, A., Am-in, S. et al. Evaluation of thermotolerant and ethanol-tolerant Saccharomyces cerevisiae as an alternative strain for bioethanol production from industrial feedstocks. 3 Biotech 13, 23 (2023). https://doi.org/10.1007/s13205-022-03436-4

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