Abstract
A new deep-sea-derived actinomycete 12A22 was isolated from the sediment of the South China Sea which showed potential cytotoxic and antimicrobial activities. The actinomycete was identified as Actinoalloteichus cyanogriseus by investigating morphological characteristics and phylogenetic analyses based on its 16S rRNA gene sequence. Two compounds, cyclo-(L-Pro-D-Pro-L-Tyr-L-Tyr) (1) and 2-hydroxyethyl-3-methyl-1,4-naphthoquinone (2), were isolated and characterized from the fermentation broth of the strain 12A22. Compound 2 exhibited significant inhibitory activities against a variety of phytopathogenic fungi (Fusarium oxysporum f. sp. cucumerinum, Setosphaeria turcica, and Botrytis cinerea) and Gram-positive bacterium (Bacillus subtilis). In particular, this compound showed better antifungal activity against Botrytis cinerea than positive control amphotericin B. Besides, compound 2 showed moderate cytotoxic activity against human breast cancer MDA-MB-435 cells with IC50 10.59 µM, weaker than the positive control diaminedichloroplatinum with 5.91 μM. Our results suggested that this naphthoquinone could be used as a potential antimicrobial and antitumor agent.
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Acknowledgements
This research was supported by the National Natural Science Foundation of China (31872036, 41776178, and 41576136), the LiaoNing Revitalization Talents Program (XLYC1807268), the Science and Technology Innovation Program for the Youth Talents of Shenyang (RC170266), and the Youth Innovation Promotion Association CAS (2018229).
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This research was supported by the National Natural Science Foundation of China (31872036, 41776178 and 41576136), the LiaoNing Revitalization Talents Program (XLYC1807268), the Science and Technology Innovation Program for the Youth Talents of Shenyang (RC170266), and the Youth Innovation Promotion Association CAS (2018229).
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HP designed and conducted the experiments, and analyzed the data. XZ and CS participated in the bioactivity assays and data analysis. XZ and HP wrote the manuscript. YB and JH revised the manuscript. All authors reviewed and approved the manuscript.
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Zhang, X., Song, C., Bai, Y. et al. Cytotoxic and antimicrobial activities of secondary metabolites isolated from the deep-sea-derived Actinoalloteichus cyanogriseus 12A22. 3 Biotech 11, 283 (2021). https://doi.org/10.1007/s13205-021-02846-0
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DOI: https://doi.org/10.1007/s13205-021-02846-0