Abstract
Mushrooms are ubiquitous in nature. Even though humankind has been consuming mushrooms for ages, their medicinal and nutraceutical properties are not used to its fullest potential in the present market. Edible mushrooms are not only a cheap and nutritious option to mitigate malnutrition, but they also produce effective biomass. Submerged fermentation (SmF) is not only a cost-effective method to produce biomass along with exquisite bioactive metabolites but it also reduces the chances of contamination and the time of production. Therefore, this study unveils the bioactive metabolites being produced by mushrooms. Moreover, it also showcases the recent advances in the areas of bio-active compounds and their judicious implementations in daily life and pharmaceutical industries. Moreover, there is a distinct lack in utilizing the potential benefits of bioactive compounds from mushroom unless in vivo and in vitro studies are demonstrated.
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References
Ahmad Wani B, Bodha R, Wani AH (2010) Nutritional and medicinal importance of mushrooms. J Med Plants Res 4:2598–2604. https://doi.org/10.5897/jmpr09.565
Akindahunsi AA, Oyetayo FL (2006) Nutrient and antinutrient distribution of edible mushroom, Pleurotus tuber-regium (fries) singer. LWT Food Sci Technol 39:548–553. https://doi.org/10.1016/j.lwt.2005.04.005
Ayer WA, Browne LM (1981) Terpenoid metabolites of mushrooms and related basidiomycetes. Tetrahedron 37:2197–2248. https://doi.org/10.1016/S0040-4020(01)97979-7
Badalyan SM (2014) Potential of mushroom bioactive molecules to develop healthcare biotech products. In: Proceedings of the 8th international conference on mushroom biology and mushroom products. pp 373–378
Benkeblia N (2015) Ganoderma lucidum polysaccharides and terpenoids: profile and health benefits. J Food Nutr Diet 01:1–6. https://doi.org/10.19104/jfnd.2015.101
Cao XY, Liu JL, Yang W et al (2015) Antitumor activity of polysaccharide extracted from Pleurotus ostreatus mycelia against gastric cancer in vitro and in vivo. Mol Med Rep 12:2383–2389. https://doi.org/10.3892/mmr.2015.3648
Chen S, Li X, Yong T et al (2017) Cytotoxic lanostane-type triterpenoids from the fruiting bodies of Ganoderma lucidum and their structure-activity relationships. Oncotarget 8:10071–10084. https://doi.org/10.18632/oncotarget.14336
Chiu CH, Chyau CC, Chen CC et al (2014) Polysaccharide extract of Cordyceps sobolifera attenuates renal injury in endotoxemic rats. Food Chem Toxicol 69:281–288. https://doi.org/10.1016/j.fct.2014.04.009
Cho EJ, Oh JY, Chang HY, Yun JW (2006) Production of exopolysaccharides by submerged mycelial culture of a mushroom Tremella fuciformis. J Biotechnol 127:129–140. https://doi.org/10.1016/j.jbiotec.2006.06.013
Cui FJ, Chen XX, Liu WM et al (2016) Control of Grifola frondosa morphology by agitation and aeration for improving mycelia and exo-polymer production. Appl Biochem Biotechnol 179:459–473. https://doi.org/10.1007/s12010-016-2006-y
Devi KSP, Roy B, Patra P et al (2013) Characterization and lectin microarray of an immunomodulatory heteroglucan from Pleurotus ostreatus mycelia. Carbohydr Polym 94:857–865. https://doi.org/10.1016/j.carbpol.2013.02.017
Elisashvili V (2012) Submerged cultivation of medicinal mushrooms: bioprocesses and products (review). Int J Med Mushrooms 14:211–239. https://doi.org/10.1615/IntJMedMushr.v14.i3.10
Elisashvili V, Penninckx M, Kachlishvili E et al (2007) Lentinus edodes and Pleurotus species lignocellulolytic enzymes activity in submerged and solid-state fermentation of lignocellulosic wastes of di V erent composition. Bioresour Technol 99:457–462. https://doi.org/10.1016/j.biortech.2007.01.011
El-Enshasy HA, Hatti-Kaul R (2013) Mushroom immunomodulators: Unique molecules with unlimited applications. Trends Biotechnol 31:668–677. https://doi.org/10.1016/j.tibtech.2013.09.003
Faccin LC, Benati F, Rincão VP et al (2007) Antiviral activity of aqueous and ethanol extracts and of an isolated polysaccharide from Agaricus brasiliensis against poliovirus type 1. Lett Appl Microbiol 45:24–28. https://doi.org/10.1111/j.1472-765X.2007.02153.x
Feng YL, Li WQ, Wu XQ et al (2010) Statistical optimization of media for mycelial growth and exo-polysaccharide production by Lentinus edodes and a kinetic model study of two growth morphologies. Biochem Eng J 49:104–112. https://doi.org/10.1016/j.bej.2009.12.002
Ferreira ICFR, Barros L, Abreu RMV (2009) Antioxidants in wild mushrooms. Curr Med Chem 16:1543–1560. https://doi.org/10.2174/092986709787909587
García-Cruz F, Durán-Páramo E, Garín-Aguilar MA et al (2020) Parametric characterization of the initial pH effect on the polysaccharides production by Lentinula edodes in submerged culture. Food Bioprod Process 119:170–178. https://doi.org/10.1016/j.fbp.2019.10.016
Gupta S, Summuna B, Gupta M, Annepu SK (2018) Edible mushrooms: cultivation, bioactive molecules, and health benefits. Bioact Mol Food. https://doi.org/10.1007/978-3-319-78030-6_86
Han JW, Oh M, Lee YJ et al (2018) Crinipellins A and I, two diterpenoids from the basidiomycete fungus Crinipellis rhizomaticola, as potential natural fungicides. Molecules 23:1–11. https://doi.org/10.3390/molecules23092377
Hoa HT, Wang C-L (2015) The effects of temperature and nutritional conditions on mycelium growth of two oyster mycobiology the effects of temperature and nutritional conditions on mycelium growth of two oyster mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus). Mycobiology 43:14–23. https://doi.org/10.5941/MYCO.2015.43.1.14
Di Hsu K, Wu SP, Lin SP et al (2017) Enhanced active extracellular polysaccharide production from Ganoderma formosanum using computational modeling. J Food Drug Anal 25:804–811. https://doi.org/10.1016/j.jfda.2016.12.006
Hwang HJ, Kim SW, Choi JW, Yun JW (2003) Production and characterization of exopolysaccharides from submerged culture of Phellinus linteus KCTC 6190. Enzyme Microb Technol 33:309–319. https://doi.org/10.1016/S0141-0229(03)00131-5
Isaka M, Palasarn S, Sommai S et al (2017) Lanostane triterpenoids from the edible mushroom Astraeus asiaticus. Tetrahedron 73:1561–1567. https://doi.org/10.1016/j.tet.2017.01.070
Jiaojiao Z, Fen W, Kuanbo L et al (2018) Heat and light stresses affect metabolite production in the fruit body of the medicinal mushroom Cordyceps militaris. Appl Microbiol Biotechnol 102:4523–4533. https://doi.org/10.1007/s00253-018-8899-3
Kalač P (2012) A review of chemical composition and nutritional value of wild-growing and cultivated mushrooms. J Sci Food Agric 93:209–218
Kawagishi H, Nomura A, Mizuno T et al (1990) Isolation and characterization of a lectin from Grifola frondosa fruiting bodies. BBA Gen Subj 1034:247–252. https://doi.org/10.1016/0304-4165(90)90045-X
Khalili M, Ebrahimzadeh MA, Kosaryan M et al (2015) Iron chelation and liver disease healing activity of edible mushroom (Cantharellus cibarius), in vitro and in vivo assays. RSC Adv 5:4804–4810. https://doi.org/10.1039/c4ra11561a
Khan A, Tania M (2012) Nutritional and medicinal importance of pleurotus mushrooms : an overview. Food Rev Int 28:313–329. https://doi.org/10.1080/87559129.2011.637267
Kim SW, Hwang HJ, Xu CP et al (2003) Effect of aeration and agitation on the production of mycelial biomass and exopolysaccharides in an enthomopathogenic fungus Paecilomyces sinclairii. Lett Appl Microbiol 36:321–326
Le J, Hu S, Xu M (2007) Optimisation of submerged culture conditions for the production of mycelial biomass and exopolysaccharide by Pleurotus nebrodensis. Ann Microbiol 57:389–393. https://doi.org/10.1007/BF03175078
Leathers TD, Nunnally MS, Stanley AM, Rich JO (2016) Utilization of corn fiber for production of schizophyllan. Biomass Bioenergy J 95:132–136. https://doi.org/10.1016/j.biombioe.2016.10.001
Lee BC, Bae JT, Pyo HB et al (2004) Submerged culture conditions for the production of mycelial biomass and exopolysaccharides by the edible Basidiomycete Grifola frondosa. Enzyme Microb Technol 35:369–376. https://doi.org/10.1016/j.enzmictec.2003.12.015
Lee C-Y, Park J-E, Kim B-B et al (2009) Determination of mineral components in the cultivation substrates of edible mushrooms and their uptake into fruiting bodies. Korean Soc Mycol 37:109–113. https://doi.org/10.4489/myco.2009.37.2.109
Lee I-S, Kirschner R, Chen S-T (2019) Medicinal mushroom Taiwanofungus camphoratus: a potential cure for cancer. Funct Foods Heal Dis 9:508–520. https://doi.org/10.31989/ffhd.v9i8.576
Li Q, Zheng Y, Zhou X (2019) Fungal immunomodulatory proteins : characteristic, potential antitumor activities and their molecular mechanisms. Drug Discov Today 24:307–314. https://doi.org/10.1016/j.drudis.2018.09.014
Lin ES, Sung SC (2006) Cultivating conditions influence exopolysaccharide production by the edible Basidiomycete Antrodia cinnamomea in submerged culture. Int J Food Microbiol 108:182–187. https://doi.org/10.1016/j.ijfoodmicro.2005.11.010
Liu J, Du C, Wang Y, Yu Z (2015) Anti-fatigue activities of polysaccharides extracted from Hericium erinaceus. Exp Ther Med 9:483–487. https://doi.org/10.3892/etm.2014.2139
Liu SR, Zhang WR (2019) Optimization of submerged culture conditions involving a developed fine powder solid seed for exopolysaccharide production by the medicinal mushroom Ganoderma lucidum. Food Sci Biotechnol 28:1135–1145. https://doi.org/10.1007/s10068-018-0536-5
Manzi P, Pizzoferrato L (2000) Beta-glucans in edible mushrooms. Food Chem 68:315–318. https://doi.org/10.1016/S0308-8146(99)00197-1
Marquez-Rocha FJ, Guillén GK, Sánchez JE, Vazquez-Duhalt R (1999) Growth characteristics of Pleurotus ostreatus in bioreactors. Biotechnol Tech 13:29–32. https://doi.org/10.1023/A:1008861432337
Martin AM (1983) Submerged production of Agaricus campestris mycellum in peat extracts. J Food Sci 48:206–207. https://doi.org/10.1111/j.1365-2621.1983.tb14824.x
Mikiashvili N, Wasser SP, Nevo E, Elisashvili V (2006) Effects of carbon and nitrogen sources on Pleurotus ostreatus ligninolytic enzyme activity. World J Microbiol Biotechnol 22:999–1002. https://doi.org/10.1007/s11274-006-9132-6
Mizuno T (1995) Bioactive biomolecules of mushrooms: food function and medicinal effect of mushroom fungi. Food Rev Int 11:7–21. https://doi.org/10.1080/87559129509541017
Oba K, Kobayashi M, Matsui T et al (2009) Individual patient based meta-analysis of lentinan for unresectable/recurrent gastric cancer. Anticancer Res 29:2739–2745
Petre M, Petre V, Teodorescu A, Patrulescu F (2013) Nutritive mushroom biomass producing through submerged fermentation of agricultural organic wastes. Stud UBB Ambientum 58(1–2):79–86
Phan C, David P, Sabaratnam V (2017) Edible and medicinal mushrooms: emerging brain food for the mitigation of neurodegenerative diseases. J Med Food 20:1–10. https://doi.org/10.1089/jmf.2016.3740
Pushparajah V, Fatima A, Chong CH et al (2016) Characterisation of a new fungal immunomodulatory protein from tiger milk mushroom, Lignosus rhinocerotis. Sci Rep 6:1–12. https://doi.org/10.1038/srep30010
Ribeiro B, de Pinho PG, Andrade PB et al (2011) Do bioactive carotenoids contribute to the color of edible mushrooms? Open Chem Biomed Methods J 4:14–18
Roupas P, Keogh J, Noakes M et al (2012) The role of edible mushrooms in health: evaluation of the evidence. J Funct Foods 4:687–709. https://doi.org/10.1016/j.jff.2012.05.003
Royse DJ, Baars J, Tan Q (2017) Current overview of mushroom production in the world. Edible Med Mushrooms Technol Appl. https://doi.org/10.1002/9781119149446.ch2
Shi X, Liu L, Gao J, Zhang A (2011) Cyathane diterpenes from Chinese mushroom Sarcodon scabrosus and their neurite outgrowth-promoting activity. Eur J Med Chem 46:3112–3117. https://doi.org/10.1016/j.ejmech.2011.04.006
Sillapachaiyaporn C, Chuchawankul S (2019) HIV-1 protease and reverse transcriptase inhibition by tiger milk mushroom (Lignosus rhinocerus) sclerotium extracts: in vitro and in silico studies. J Tradit Complement Med. https://doi.org/10.1016/j.jtcme.2019.08.002
Subramaniyam R, Vimala R (2012) Solid state and submerged fermentation for the production of bioactive substances: a comparative study. Int J Sci Nat 3:480–486
Sullivan R, Smith JE, Rowan NJ (2006) Medicinal mushrooms and cancer therapy: translating a traditional practice into Western medicine. Perspect Biol Med 49:159–170. https://doi.org/10.1353/pbm.2006.0034
Tang Y, Zhu L, Li H, Li D (2007) Submerged culture of mushrooms in bioreactors—challenges, current state-of-the-art, and future prospects. Food Technol Biotechnol 45:221–229
Tsukamoto S, Macabalang AD, Nakatani K et al (2003) Tricholomalides A–C, new neurotrophic diterpenes from the mushroom Tricholoma sp. J Nat Prod 66:1578–1581
Vacharathit V (2014) Production of provitamina carotenoids in mushrooms and uses thereof. US Patent 2:1–17
Vamanu E (2012) Biological activities of the polysaccharides produced in submerged culture of two edible Pleurotus ostreatus mushrooms. J Biomed Biotechnol 2012:1–7. https://doi.org/10.1155/2012/565974
Volman JJ, Mensink RP, Van Griensven LJLD, Plat J (2010) Effects of α-glucans from Agaricus bisporus on ex vivo cytokine production by LPS and PHA-stimulated PBMCs; a placebo-controlled study in slightly hypercholesterolemic subjects. Eur J Clin Nutr 64:720–726. https://doi.org/10.1038/ejcn.2010.32
Wan JM (2013) Polysaccaride krestin (PSK) and polysaccharopeptide PSP, 2nd edn. Elsevier Inc, Amsterdam
Wang H, Gao J, Ng TB (2000) A new lectin with highly potent antihepatoma and antisarcoma activities from the oyster mushroom Pleurotus ostreatus. Biochem Biophys Res Commun 275:810–816. https://doi.org/10.1006/bbrc.2000.3373
Wang HX, Ng TB, Liu WK et al (1995) Isolation and characterization of two distinct lectins with antiproliferative activity from the cultured mycelium of the edible mushroom Tricholoma mongolicum. Int J Pept Protein Res 46:508–513. https://doi.org/10.1111/j.1399-3011.1995.tb01606.x
Wang P-H, Hsu C-I, Tang S-C et al (2004) Fungal immunomodulatory protein from flammulina velutipes induces interferon-γ production through p38 mitogen-activated protein kinase signaling pathway. J Agric Food Chem 52:2721–2725. https://doi.org/10.1021/jf034556s
Wang S, Bao L, Zhao F et al (2013) Isolation, identification, and bioactivity of monoterpenoids and sesquiterpenoids from the mycelia of edible mushroom Pleurotus cornucopiae. J Agric Food Chem 61:5122–5129. https://doi.org/10.1021/jf401612t
Wang X, Su K, Bao T et al (2012) Immunomodulatory effects of fungal proteins. Curr Top Nutraceutical Res 10:1–12
Wang Y, Zhang Y, Shao J et al (2019) Potential immunomodulatory activities of a lectin from the mushroom Latiporus sulphureus. Int J Biol Macromol 130:399–406. https://doi.org/10.1016/j.ijbiomac.2019.02.150
Wasser SP (2002) Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl Microbiol Biotechnol 60:258–274. https://doi.org/10.1007/s00253-002-1076-7
Wei J, Guo WH, Cao CY et al (2018) Polyoxygenated cyathane diterpenoids from the mushroom Cyathus africanus, and their neurotrophic and anti-neuroinflammatory activities. Sci Rep 8:1–15. https://doi.org/10.1038/s41598-018-20472-4
Weng CJ, Chau CF, Chen KD et al (2007) The anti-invasive effect of lucidenic acids isolated from a new Ganoderma lucidum strain. Mol Nutr Food Res 51:1472–1477. https://doi.org/10.1002/mnfr.200700155
Wu Y, Choi MH, Li J et al (2016) Mushroom cosmetics: the present and future. Cosmetics 3:1–13. https://doi.org/10.3390/cosmetics3030022
Xu DQ, Fu ML, Chen QH, Liu J (2011a) Effect of submerged culture conditions on exopolysaccharides production by Armillaria luteo-virens Sacc QH and kinetic modeling. Bioprocess Biosyst Eng 34:103–111. https://doi.org/10.1007/s00449-010-0451-5
Xu X, Yan H, Chen J, Zhang X (2011b) Bioactive proteins from mushrooms. Biotechnol Adv 29:667–674. https://doi.org/10.1016/j.biotechadv.2011.05.003
Yaoita Y, Kikuchi M, Machida K (2014) Terpenoids and sterols from some Japanese Mushrooms Yasunori. NPC Nat Prod Commun 9:419–426. https://doi.org/10.1177/1934578X1400900332
Zhang B-B, Guan Y-Y, Hu P-F et al (2019) Production of bioactive metabolites by submerged fermentation of the medicinal mushroom Antrodia cinnamomea: recent advances and future development. Crit Rev Biotechnol 39:541–554. https://doi.org/10.1080/07388551.2019.1577798
Zhang G, Sun J, Wang H, Ng TB (2010) First isolation and characterization of a novel lectin with potent antitumor activity from a Russula mushroom. Phytomedicine 17:775–781. https://doi.org/10.1016/j.phymed.2010.02.001
Zhao Z, Chen H, Wu B et al (2017) Matsutakone and matsutoic acid, two (Nor) steroids with unusual skeletons from the edible mushroom Tricholoma matsutake. J Org Chem. https://doi.org/10.1021/acs.joc.7b01230
Zou X, Sun M, Guo X (2006) Quantitative response of cell growth and polysaccharide biosynthesis by the medicinal mushroom Phellinus linteus to NaCl in the medium. World J Microbiol Biotechnol 22:1129–1133. https://doi.org/10.1007/s11274-006-9153-1
Acknowledgements
The authors would like to thank Indian Institute of Technology-Hyderabad for providing the required facilities. The work is funded by Science and Engineering Research Board, Department of Science and Technology (SERB/EEQ/2018/000131). The authors are also grateful to the members of IBBL for their continuous support and efforts. The first author would also like to extend her sincere gratitude to her husband Mr. Haji Mastan and her family for their unconditional support and faith in her.
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UTD: conceptualization, methodology, data curation, writing—original draft preparation, investigation. KD: data curation, validation. Dr. DSK: visualization, writing—reviewing and editing, supervision.
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Dudekula, U.T., Doriya, K. & Devarai, S.K. A critical review on submerged production of mushroom and their bioactive metabolites. 3 Biotech 10, 337 (2020). https://doi.org/10.1007/s13205-020-02333-y
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DOI: https://doi.org/10.1007/s13205-020-02333-y