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Volatile emission compounds from plant growth-promoting bacteria are responsible for the antifungal activity against F. solani

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Abstract

The aims of this work were to screen isolated bacteria with a dual capacity: to inhibit Fusarium solani and to promote plant growth. Also, volatile compounds that would be responsible for that effect were identified. Seventy bacterial strains from the air, agricultural soils, hydrocarbons-contaminated soils, and extremophile soils were tested. The former were identified by Matrix-Assisted Laser Desorption/Ionization-time of flight mass spectrometry and 16S rDNA sequencing. The plant growth-promoting bacteria (PGPB) and their capability for phosphate solubilization, siderophores production, and indole production were determined. Twenty isolates from Bacillus and Pseudomonas genera inhibited the mycelial growth up to 40% in direct assays. Eleven isolates significantly inhibited mycelial growth in 18–24% via volatile emissions. Volatile compounds related to antifungal activity or stress response include ketones, sesquiterpenes, monoterpenoids, alkanes, and fatty acids. Our results support the potential of these PGPB to act as biocontrol agents against fungal pathogens via volatile emissions.

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Acknowledgements

A. Gutierrez-Santa Ana acknowledges the support through fellowship (720742) of Consejo Nacional de Ciencia y Tecnología (CONACYT), Mexico.

Funding

The present study was funded by the Fondo Institucional de Fomento Regional para el Desarrollo Científico, Tecnológico y de Innovación (FORDECYT) under the project 292399.

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Authors and Affiliations

  1. Unidad de Tecnología Ambiental, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), Av. Normalistas No. 800, Col. Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico

    Andrea Gutiérrez-Santa Ana, H. A. Carrillo-Cerda & S. M. Contreras-Ramos

  2. Unidad de Servicios Analíticos y Metrológicos, CIATEJ, Av. Normalistas No. 800, Col. Colinas de la Normal, 44270, Guadalajara, Mexico

    J. Rodriguez-Campos

  3. Unidad de Tecnología Industrial, CIATEJ, Camino Arenero No. 1227, El Bajío, 45019, Zapopan, Jalisco, Mexico

    M. R. Kirchmayr

  4. Catedra-Conacyt assigned to Unidad de Tecnología Ambiental at CIATEJ, Av. Normalistas No. 800, Col. Colinas de la Normal, 44270, Guadalajara, Mexico

    J. B. Velázquez-Fernández

Authors
  1. Andrea Gutiérrez-Santa Ana
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  2. H. A. Carrillo-Cerda
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  3. J. Rodriguez-Campos
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  4. M. R. Kirchmayr
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  5. S. M. Contreras-Ramos
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  6. J. B. Velázquez-Fernández
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Contributions

Conceptualization and experimental design were made by SMC-R and JBV-F. Experiments were mainly performed by AG-SA with the guidance, experiment design and help of JR-C (for chromatographical analysis and compound identification), MK (for MALDI-TOF identificaction) and JBV-F (MALDI-TOF identification, 16S identification and statistical analysis). HAC-C helped with microbial culture and confrontation of bacteria against F. solani. First draft was written by AG-SA and all authors read, revised, commented on previous versions. All authors whose names appear on the submission. (1) made substantial contributions to the design of the work, the acquisition, analysis, or interpretation of data; (2) approved the version to be published; and (3) agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

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Correspondence to J. B. Velázquez-Fernández.

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Gutiérrez-Santa Ana, A., Carrillo-Cerda, H.A., Rodriguez-Campos, J. et al. Volatile emission compounds from plant growth-promoting bacteria are responsible for the antifungal activity against F. solani. 3 Biotech 10, 292 (2020). https://doi.org/10.1007/s13205-020-02290-6

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