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Investigating the endophytic bacterial diversity and community structures in seeds of genetically related maize (Zea mays L.) genotypes

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Abstract

This research aimed to investigate the composition and diversity of endophytic bacterial community in seeds of four hybrid maize and their parental lines, which was used to reveal the potential relationship and association of endophytic bacteria between maize genotypes and their genetic relevance. High-throughput sequencing (HTS) technology showed that a total of 1419 OTUs (46.6%) were parental lines unique and 1052 OTUs (34.5%) were hybrid varieties unique, with only 575 core OTUs revealed in all the samples. Most OTUs belonged to Proteobacteria. Enterobacter (23.2%), Shigella (21.2%), Pseudomonas (15.8%) and Achromobacter (10.1%) were the major genera; the bacterial community composition and diversity of endophytic bacteria were inconsistent among different seed genotypes. Based on principal component analysis (PCA), the results referred that the endophytic composition of hybrid sample showed obvious correlation with their female parental lines, and in ‘Jingke968’ and ‘MC738’ with the same female line the endophytic community was more similar than other hybrid samples. This was the first ever use of HTS technology for investigating the endophytic bacterial diversity and community structures in seeds of genetically related maize genotypes, it was shown that, there were core microbes shared among all genotypes of seed samples, and the female parental line was more significant to impact on the composition of their hybrid seeds than male parental line. This study would provide scientific clues for the future research on the vertical transmission of endophytes among maize generations.

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Acknowledgements

We thank Dr. Shahbaz Ahmad at University of Science and Technology Beijing and Miss Xiaoxiao Cheng at New Mexico State University (USA) for assistance with English language and grammatical editing of the manuscript.

Funding

This work was supported by the Natural Science Foundation of Beijing, China (no. 6182008), the Fundamental Research Funds for Central Non-profit Scientific Institution (no. 1610132017041) and the Fundamental Research Funds for the Central Universities (no. FRF-TP-18-012A1; FRF-BR-18-009B).

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Authors and Affiliations

  1. College of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China

    Yang Liu, Hai Yan, Miao Li & Fuzhen Yang

  2. Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Beijing, 100081, China

    Xiaoxia Zhang

  3. Maize Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China

    Ruyang Zhang, Tianjun Xu & Jiuran Zhao

  4. Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai, 201203, China

    Huajun Zheng

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  1. Yang Liu
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Liu, Y., Yan, H., Zhang, X. et al. Investigating the endophytic bacterial diversity and community structures in seeds of genetically related maize (Zea mays L.) genotypes. 3 Biotech 10, 27 (2020). https://doi.org/10.1007/s13205-019-2034-8

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