Abstract
This study investigated the potential of a new material, so-called small-flowered nutsedge, for bioethanol production. This plant causes the huge loss of rice yield as it competes nutrients, sunlight and other necessary elements with rice plant to grow. The project plans to transform its biomass into valuable product and bring profitable for famers. The powered raw sample was treated with sodium hydroxide (NaOH) and hydrogen peroxide (H2O2) as followed the design experiment. The use of response surface method is helping researcher to save time and effort but still gain meaningful predicted value that closed to the actual value. The highest total sugar was given when the pretreatment condition is solid to liquid ratio of 0.05, 1% NaOH, 1% H2O2 for 72 h. The efficiency of hydrolysis can reach 47% after 24 h with cellulase enzyme at 50 °C, 150 rpm and highest ethanol concentration was obtained on the fifth day of fermentation.
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References
Ahn DJ, Kim SK, Yun HS (2012) Optimization of pretreatment and saccharification for the production of bioethanol from water hyacinth by Saccharomyces cerevisiae. Bioprocess Biosyst Eng 35(1–2):35–41
Antony J (2014) Design of experiments for engineers and scientists, 2nd edn. Elsevier, London
Aswathy US, Sukumaran RK, Devi GL, Rajasree KP, Singhania RR, Pandey A (2010) Bio-ethanol from water hyacinth biomass: an evaluation of enzymatic saccharification strategy. Bioresour Technol 101(3):925–930
Ayeni AO, Hymore FK, Mudliar SN, Deshmukh SC, Satputea DB, Omoleye JA, Pandey RA (2013) Hydrogen peroxide and lime based oxidative pretreatment of wood waste to enhance enzymatic hydrolysis for a biorefinery: process parameters optimization using response surface methodology. Fuel 106:187–194
Box GEP, Behnken DW (1960) Some new three level designs for the study of quantitative variables. Technometrics 2(4):455–475
Cai BY, Ge JP, Ling HZ, Cheng KK, Ping WX (2012) Statistical optimization of dilute sulfuric acid pretreatment of corncob for xylose recovery and ethanol production. Biomass Bioenergy 36:250–257
Chauhan B, Johnson D (2009) Ecological studies on Cyperus difformis, Cyperus iria and Fimbristylis miliacea: three troublesome annual sedge weeds of rice. Ann Appl Biol 155(1):103–112
Das S, Bhattacharya A, Haldar S, Ganguly A, Gu S, Ting YP, Chatterjee PK (2015) Optimization of enzymatic saccharification of water hyacinth biomass for bio-ethanol: comparison between artificial neural network and response surface methodology. Sustain Mater Technol 3:17–28
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28(3):350–356
Guan X, Yao H (2008) Optimization of Viscozyme L-assisted extraction of oat bran protein using response surface methodology. Food Chem 106(1):345–351
Hendriks ATWM, Zeeman G (2009) Pretreatments to enhance the digestibility of lignocellulosic biomass. Bioresour Technol 100(1):10–18
Kang Q, Appels L, Tan T, Dewil R (2014) Bioethanol from lignocellulosic biomass: current findings determine research priorities. Sci World J 2014:298153
Kuk YI, Kim KH, Kwon OD, Lee DJ, Burgos NR, Jung S, Guh JO (2004) Cross-resistance pattern and alternative herbicides for Cyperus difformis resistant to sulfonylurea herbicides in Korea. Pest Manag Sci 60(1):85–94
Küüt A (2013) Characteristics of bioethanol fuel obtained from lignocellulose biomass in internal combustion reciprocating engines with spark- and compression-ignition. Estonian University of Life Sciences, Tartu, pp 25–28
Mäkelä M (2017) Experimental design and response surface methodology in energy applications: a tutorial review. Energy Convers Manag 151:630–640
Montgomery D, Runger G (2002) Applied Probability and Statistics to Engineering. McGraw Hill, Mexico
Rawat R, Kumbhar BK, Tewari L (2013) Optimization of alkali pretreatment for bioconversion of poplar (Populus deltoides) biomass into fermentable sugars using response surface methodology. Ind Crops Prod 44:220–226
Saha BC, Cotta MA (2006) Ethanol production from alkaline peroxide pretreated enzymatically saccharified wheat straw. Biotechnol Prog 22(2):449–453
Singh R, Krishna BB, Kumar J, Bhaskar T (2016) Opportunities for utilization of non-conventional energy sources for biomass pretreatment. Bioresour Technol 199:398–407
Tarkow H, Feist WC (1969) A mechanism for improving the digestibility of lignocellulosic materials with dilute alkali and liquid ammonia. ACS Publications, Washington D. C.
Vu PT, Unpaprom Y, Ramaraj R (2017) Evaluation of bioethanol production from rice field weed biomass. ELSR 3(2):42–49
Vu PT, Unpaprom Y, Ramaraj R (2018) Impact and significance of alkaline-oxidant pretreatment on the enzymatic digestibility of Sphenoclea zeylanica for bioethanol production. Bioresour Technol 247:125–130
Witek-Krowiak A, Chojnacka K, Podstawczyk D, Dawiec A, Pokomeda K (2014) Application of response surface methodology and artificial neural network methods in modelling and optimization of biosorption process. Bioresour Technol 160:150–160
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I thank the Faculty of Science, Maejo University, to provide the facility to conduct this research, Ms. Phu Thi Vu and Mrs. Sawitree Tipanee for laboratory assistant.
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Ramaraj, R., Unpaprom, Y. Optimization of pretreatment condition for ethanol production from Cyperus difformis by response surface methodology. 3 Biotech 9, 218 (2019). https://doi.org/10.1007/s13205-019-1754-0
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DOI: https://doi.org/10.1007/s13205-019-1754-0