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Functional characterization of the rice root Germin-like protein gene-1 (OsRGLP1) promoter in Nicotiana tabacum

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Abstract

Germin (GER) and germin-like protein (GLP) genes play a very important role against various stresses. Promoter analysis provide significant insight into gene’s function and regulation. Presently, upstream region (1228 bp) of the OsRGLP1 gene was functionally characterized via heterologous expression. It was fused with the Glucuronidase (GUS) reporter gene and the expression cassette was used to transform Nicotiana tabacum using Agrobacterium-mediated transformation. Transgenic plants were examined via quantitative real-time PCR (qPCR) to analyze its role in wounding, salinity, drought, abscisic acid (ABA) and circadian rhythm. OsRGLP1 was highly induced by ABA and drought by showing 28- and 25-fold changes in GUS mRNA level respectively as compared to wounding (fourfold change) and salinity (threefold change). However, no activity was observed in circadian rhythm. Histochemically, strong GUS activity was observed in leaf veins, midrib, epidermal hair, stomata guard cells, stem cortex, root hairs, xylem and phloem and at cellular level in cell wall, cytoplasm and its periphery. OsRGLP1 promoter can be used to develop agronomically important transgenic plants in future food program.

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Acknowledgements

We are thankful to The Higher Education Commission (HEC) of Pakistan for providing financial assistance. Grant no.: 17-5-(2BM1-351)HEC/Sch-ind/2012.

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Authors and Affiliations

  1. Department of Botany, University of Swabi, Anbar Swabi, 23561, Khyber Pakhtunkhwa, Pakistan

    Muhammad Ilyas

  2. Department of Botany, Faculty of Biological sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan

    Tariq Mahmood

  3. Bacha Khan University, Charsadda, KP, Pakistan

    Syed Muhammad Saqlan Naqvi

  4. Department of Botany, Mohi-Ud-Din Islamic University, Nerian Sharif, Azad Jammu and Kashmir, Pakistan

    Wasim Akhtar

  5. Department of Botany, University of Gujrat sub campus Rawalpindi, Rawalpindi, Pakistan

    Shazia Rehman

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  1. Muhammad Ilyas
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  2. Wasim Akhtar
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  3. Shazia Rehman
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  4. Syed Muhammad Saqlan Naqvi
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  5. Tariq Mahmood
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Contributions

MI and TM conducted experiments while SR, WA and SMSN helped in manuscript writing.

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Correspondence to Muhammad Ilyas.

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The authors declared that they have no conflict of interest in the publication.

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Ilyas, M., Akhtar, W., Rehman, S. et al. Functional characterization of the rice root Germin-like protein gene-1 (OsRGLP1) promoter in Nicotiana tabacum. 3 Biotech 9, 130 (2019). https://doi.org/10.1007/s13205-019-1644-5

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  • DOI: https://doi.org/10.1007/s13205-019-1644-5

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