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Moderate levels of glyphosate and its formulations vary in their cytotoxicity and genotoxicity in a whole blood model and in human cell lines with different estrogen receptor status

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Abstract

In vitro studies were conducted to determine the short-term cytotoxic and genotoxic effects of pure glyphosate and two glyphosate formulations (Roundup® and Wipeout®) at concentrations relevant to human exposure using whole blood (cytotoxicity) and various cancer cell lines (cytotoxicity and genotoxicity). Pure glyphosate (pure glyph) and Roundup® (Ro) showed similar non-monotonic toxicological profiles at low dose exposure (from 10 µg/ml), whereas Wipeout® (Wo) demonstrated a monotonic reduction in cell viability from a threshold concentration of 50 µg/ml, when tested in whole blood. We evaluated whether using various cancer cells (the estrogen-E2-responsive HEC1A, MCF7 and the estrogen-insensitive MDA-MB-231) exposed to moderate doses (75–500 µg/ml) would indicate varied toxicity and results indicated significant effects in the HEC1A cancer cells. A non-monotonic reduction in cell viability was observed in HEC1A exposed to pure glyph (75–500 µg/ml) and proliferative effects were observed after exposure to Wo (75, 125 and 250 µg/ml). Genotoxicity assessment (test concentration 500 µg/ml) demonstrated DNA damage in the HEC1A and MDA-MB-231 cells. Adjuvants and/or glyphosate impurities were potential contributing factors of toxicity based on the differential toxicities displayed by Ro and Wo in human whole blood and the HEC1A cells. This study contributes to the existing knowledge about in vitro exposure to moderate concentrations of glyphosate or glyphosate formulations at cytotoxic and genotoxic levels. In addition, a suggestion on the relevance of the estrogen receptor status of the cell lines used is provided, leading to the need to further investigate a potential endocrine disruptive role.

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Abbreviations

EPSP:

5-Enolpyruvylshikimate-3-phosphate synthase

EPA:

Environmental Protection Agency

ER:

Estrogen receptor

E-responsive:

Estrogen responsive

Pure glyph:

Pure glyphosate (99.5% purity)

Ro:

Roundup®

Wo:

Wipeout®

DI H2O:

Deionised water

FITC:

Fluorescein isothiocyanate

SEM:

Standard error of the mean

TM:

Olive tail moment

TL:

Tail length (µm)

TDNA:

Tail DNA (%)

WHO:

World Health Organization

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Acknowledgements

The authors would like to thank the Water Research Commission (WRC) of South Africa for funding. Conclusions drawn and opinions expressed are those of the authors and should not be attributed to the funding body. The authors wish to thank the reviewers for their valuable insight as it has improved the work.

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  1. Department of Biochemistry and Microbiology, Rhodes University, P.O. Box 94, Grahamstown, 6140, South Africa

    L. K. S. De Almeida & B. I. Pletschke

  2. Department of Biochemistry and Microbiology, Nelson Mandela University, P.O. Box 77000, Port Elizabeth, 6031, South Africa

    C. L. Frost

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  1. L. K. S. De Almeida
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De Almeida, L.K.S., Pletschke, B.I. & Frost, C.L. Moderate levels of glyphosate and its formulations vary in their cytotoxicity and genotoxicity in a whole blood model and in human cell lines with different estrogen receptor status. 3 Biotech 8, 438 (2018). https://doi.org/10.1007/s13205-018-1464-z

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