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Full-length genome sequence of Cyrtanthus elatus virus-A isolated from Narcissus tazetta in India

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Abstract

Narcissus tazetta L. is a bulbous ornamental plant popular for its notable fragrant flowers which make it the plant of high importance. In spite of its economic value, narcissus is found to be susceptible for a number of diseases borne by fungi, bacteria, nematodes, and viruses. A potyvirus, Cyrtanthus elatus virus-A isolate NBRI16 (CEVA-NBRI16), associated with leaf chlorotic stripe disease of N. tazetta cv. Paperwhite was reported for first time in India from our laboratory based on the partial coat protein gene sequence. In present study, the full-length genomic sequence of CEVA-NBRI16 is determined which consists of 9942 nucleotides, excluding the polyA tail, and encodes a single large polyprotein of 3102 amino acids with the genomic features typical of a potyvirus. It shares highest 93% nucleotide sequence identity and closest phylogenetic relationship with sequences of CEVA-Marijiniup7-1 and CEVA-Marijiniup7-2, both reported from Australia on Cyrtanthus elatus host. The full-length genomic sequence of CEVA from narcissus plant is being reported for the first time from India.

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Acknowledgements

This study was funded by the Council of Scientific and Industrial Research, New Delhi, India (Grant Number BSC-0117). RR is thankful to University Grant Commission for Rajiv Ghandi National fellowship.

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Authors and Affiliations

  1. Plant Molecular Virology Laboratory, Council of Scientific and Industrial Research-National Botanical Research Institute (CSIR-NBRI), Lucknow, UP, 226001, India

    Rashmi Raj, Charanjeet Kaur & S. K. Raj

  2. Department of Plant Microbe Interactions, CSIR-NBRI, Lucknow, UP, 226001, India

    Lalit Agrawal, P. S. Chauhan & Susheel Kumar

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  1. Rashmi Raj
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  2. Charanjeet Kaur
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  3. Lalit Agrawal
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  4. P. S. Chauhan
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  5. Susheel Kumar
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  6. S. K. Raj
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Correspondence to S. K. Raj.

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Raj, R., Kaur, C., Agrawal, L. et al. Full-length genome sequence of Cyrtanthus elatus virus-A isolated from Narcissus tazetta in India. 3 Biotech 8, 168 (2018). https://doi.org/10.1007/s13205-018-1189-z

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