Abstract
Brugmansia suaveolens mottle virus (BsMoV) was the first potyvirus isolated from “angel trumpet” (Brugmansia suaveolens), described in Brazil. In this study, the complete nucleotide (nt) genome sequence of BsMoV was determined, and the deduced amino acid (aa) sequence was analyzed. The BsMoV RNA genome consists of 9870 nt without a poly-A tail, encoding a putative typical potyviral polyprotein of 3090 aa. Pairwise comparisons of the complete BsMoV genome with those of the most closely related potyviruses revealed a maximum nucleotide identity of 63.7% with pepper mottle virus. These results and phylogenetic analyses based on the complete genome sequence of the most closely related potyviruses confirmed that BsMoV should be considered a member of a distinct species of the genus Potyvirus.
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References
Adams MJ, Antoniw JF, Beaudoin F (2005) Overview and analysis of the polyprotein cleavage sites in the family Potyviridae. Mol Plant Pathol 6:471–487
Adams MJ, Antoniw JF, Fauquet CM (2005) Molecular criteria for genus and species discrimination within the family Potyviridae. Arch Virol 150:459–479
Atreya PL, Atreya CD, Pirone TP (1991) Amino-acid substitutions in the coat protein result in loss of insect transmissibility of a plant-virus. Proc Natl Acad Sci USA 88:7887–7891
Carrington JC, Cary SM, Parks TD, Dougherty WG (1989) A 2nd proteinase encoded by a plant potyvirus genome. EMBO J 8:365–370
Chen J, Chen J, Adams MJ (2001) A universal PCR primer to detect members of the Potyviridae and its use to examine the taxonomic status of several members of the family. Arch Virol 146:757–766
Chung BY, Miller WA, Atkins JF, Firth AE (2008) An overlapping essential gene in the Potyviridae. Proc Natl Acad Sci USA 105:5897–5902
Gal-On A (2000) A point mutation in the FRNK motif of the potyvirus helper component-protease gene alters symptom expression in cucurbits and elicits protection against the severe homologous virus. Phytopathology 90:467–473
Huet H, Gal-On A, Meir E, Lecoq H, Raccah B (1994) Mutations in the helper component protease gene of zucchini yellow mosaic virus affect its ability to mediate aphid transmissibility. J Gen Virol 75:1407–1414
Inokuchi Y, Hirashima A (1987) Interference with viral infection by defective RNA replicase. J Virol 61:3946–3949
Lucinda N, Nagata T, Inoue-Nagata AK, Salaroli RB, Kitajima EW (2008) Brugmansia suaveolens mottle virus, a novel potyvirus causing leaf mottling of Brugmansia suaveolens in Brazil. Arch Virol 153:1971–1976
Maia IG, Haenni A, Bernardi F (1996) Potyviral HC-Pro: a multifunctional protein. J Gen Virol 77:1335–1341
Poch O, Sauvaget I, Delarue M, Tordo N (1989) Identification of four conserved motifs among the RNA-dependent polymerase encoding elements. EMBO J 8:3867–3874
Staden R, Judge DP, Bonfield JK (2003) Managing sequencing projects in GAP4 environment. In: Krawetz SA, Womble DD (eds) Introduction to Bioinformatics: A Theoretical and pratical approach. Human Press Inc, Totawa
Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599
Thompson JD, Higgins DG, Gibson TJ (1994) Clustal-W––improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Acknowledgments
Nucleotide sequence datum is available in the DDBJ/EMBL/GenBank database under accession number AB551370.
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Lucinda, N., Inoue-Nagata, A.K., Kitajima, E.W. et al. Complete genome sequence of Brugmansia suaveolens mottle virus, a potyvirus from an ornamental shrub. Arch Virol 155, 1729–1732 (2010). https://doi.org/10.1007/s00705-010-0798-6
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DOI: https://doi.org/10.1007/s00705-010-0798-6