Abstract
Efficient in vitro propagation systems via organogenesis and synthetic seeds were developed for the first time for conservation and commercial propagation from leaf or longitudinal thin cell layer (lTCL) leaf or shoot-tip explants of Urginea altissima. Various plant growth regulators and phloroglucinol were used in semi-solid and liquid Murashige and Skoog (MS) medium to establish multiplication of shoots and roots for in vitro regeneration. Of the various treatments, the highest number of shoots (17.4 per lTCL leaf explant) was obtained on liquid MS medium supplemented with 10 µM meta-Topolin (mT) and 2 µM benzyladenine followed by transferal to semi-solid MS media. The shoot tips were encapsulated with liquid MS medium plus 3% (w/v) sodium alginate and 100 mM calcium chloride. Adventitious shoot regeneration (91.0%; 12.6 shoots per synthetic seed) of synthetic seeds was achieved on semi-solid MS medium supplemented with 10 µM mT and 2 µM naphthaleneacetic acid (NAA) after 15 days of storage in darkness at 25 ± 2 °C. Regenerated shoots rooted (9.8 roots per shoot; 6.5 cm long) efficiently when transferred to 5 µM indole-3-butyric acid and 2.5 µM NAA. All the plantlets were successfully acclimatized (100%) in a vermiculite:soil (1:1 v/v) mixture in the greenhouse.
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References
Ascough GD, Fennell CW (2004) The regulation of plant growth and development in liquid culture. S Afr J Bot 70:181–190
Baskaran P, Ncube B, Van Staden J (2012) In vitro propagation and secondary product production by Merwilla plumbea (Lindl.) Speta. Plant Growth Regul 67:235–245
Baskaran P, Singh S, Van Staden J (2013) In vitro propagation, proscillaridin A production and antibacterial activity in Drimia robusta. Plant Cell Tissue Organ Cult 114:259–267
Baskaran P, Kumari A, Van Staden J (2015) Embryogenesis and synthetic seed production in Mondia whitei. Plant Cell Tissue Organ Cult 121:205–214
El Grari R, Backhaus RA (1987) In vitro propagation of red squill, Urginea maritima Baker. Plant Cell Tissue Organ Cult 10:65–71
Fernando SC, Goodger JQD, Gutierrez SS, Johnson AAT, Woodrow IE (2016) Plant regeneration through indirect organogenesis and genetic transformation of Eucalyptus polybractea R.T. Baker. Ind Crop Prod 86:73–78
Georgiev V, Ivanov I, Berkov S, Pavlov A (2011) Alkaloids biosynthesis by Pancratium maritimum L. shoots in liquid culture. Acta Physiol Plant 33:927–933
Grzegorczyk-Karolak I, Rytczak P, Bielecki S, Wysokińska H (2017) The influence of liquid systems for shoot multiplication, secondary metabolite production and plant regeneration of Scutellaria alpine. Plant Cell Tissue Organ Cult 128:479–486
Hutchings A (1989) A survey and analysis of traditional medicinal plants as used by the Zulu, Xhosa and Sotho. Bothalia 19:111–123
Hutchings A, Scott H, Lewis G, Cunningllam AB (1996) Zulu medicinal plants-an inventory. University of Natal press, Pietermaritzburg
Islam MS, Bari MA (2012) In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L. J Biosci 20:99–108
Jha S, Sen S (1986) Mass propagation of Indian squill, Urginea indica Kunth in vitro. Acta Hortic 188:105–109
Jha S, Mitra GC, Sen S (1984) In vitro regeneration from bulb explants of Indian squill, Urginea indica Kunth. Plant Cell Tissue Organ Cult 3:91–100
Koperuncholan S, Ahmed John M (2012) Direct root regeneration and indirect organogenesis in Silybum marianum and preliminary phytochemical, antibacterial studies of its callus. Int J Pharm 2:392–400
Kumari A, Baskaran P, Chukwujekwu JC, de Kock CA, Smith BJ, Van Staden J (2016) The changes in morphogenesis and bioactivity of Tetradenia riparia, Mondia whitei and Cyanoptis speciosa by an aeroponic system. Ind Crop Prod 84:199–204
Langat LC, Westchnig W, Langat MK, Mulholland DA (2013) Novel bufadienolides from South African Urginavia altissima (Hyacinthaceae). In: Proceedings of ICNP, p 213
Manjkhola S, Dhar U, Joshi M (2005) Organogenesis, embryogenesis, and synthetic seed production in Arnebia euchroma—a critically endangered medicinal plant of the Himalaya. In Vitro Cell Dev Biol Plant 41:244–248
McCartan SA, Van Staden J (1999) Micropropagation of members of the Hyacinthaceae with medicinal and ornamental potential—a review. S Afr J Bot 65:361–369
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497
Nasircilar AG, Mirci S, Karaguzel O, Eren O, Baktir I (2011) In vitro propagation of endemic and endangered Muscari mirum from different explant types. Turk J Bot 35:37–43
Nyende AB, Schittenhelm S, Mix-Wagner G, Greef JM (2003) Production, storability, and regeneration of shoot tips of potato (Solanum tuberosum L.) encapsulated in calcium alginate hollow beads. In Vitro Cell Dev Biol Plant 39:540–549
Ray A, Bhattacharya S (2008) Storage and plant regeneration from encapsulated shoot tips of Rauvolfia serpentine—an effective way of conservation and mass propagation. S Afr J Bot 74:776–779
Rout GR, Mohapatra A, Mohan Jain S (2006) Tissue culture of ornamental pot plant: a critical review on present scenario and future prospects. Biotechnol Adv 24:531–560
Sajc L, Grubisic D, Vunjak-Novakovic G (2000) Bioreactors for plant engineering: an outlook for further research. Biochem Eng J 4:89–99
SANBI (2017) Statistics: red list of South African plants version 2017.1. http://redlist.sanbi.org/species.php?species=3812-21. Accessed 25 May 2017
Shahzad A, Shaheen A (2013) In vitro conservation protocols for some threatened medicinal-plant. In: Shahid M, Shahzad A, Malik A, Sahal A (eds) Recent trends in biotechnology and therapeutic applications of medicinal plants. Springer, Dordrecht, pp 293–304
Stojakowska A (1993) Micropropagation of Urginea maritima (L.) Baker S. STR. Acta Bot Pol 62:11–15
Verma SK, Das AK, Cingoz GS, Uslu E, Gurel E (2016) Influence of nutrient media on callus induction, somatic embryogenesis and plant regeneration in selected Turkish crocus species. Biotechnol Rep 10:66–74
Watt JM, Breyer-Brandwijk MG (1962) Medicinal and poisonous plants of southern and eastern Africa. E & S Livingstone Ltd., Edinburgh
Williams VL, Raimondo D, Crouch NR, Brueton VJ, Cunningham AB, Scott-Shaw CR, Lötter M, Ngwenya AM (2016) Drimia altissima (L.f.) Ker Gawl. National Assessment: Red List of South African Plants version 2017.1. http://redlist.sanbi.org/species.php?species=3812-21. Accessed 25 May 2017
Yanthan SJ, Kehie M, Kumaria S, Tandon P (2017) In vitro regeneration of Drosera burmannii Vahl.: a carnivorous plant of north-east India. 3 Biotech 70:124
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The financial support by the University of KwaZulu-Natal, Pietermaritzburg is gratefully acknowledged.
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Baskaran, P., Kumari, A. & Van Staden, J. In vitro propagation via organogenesis and synthetic seeds of Urginea altissima (L.f.) Baker: a threatened medicinal plant. 3 Biotech 8, 18 (2018). https://doi.org/10.1007/s13205-017-1028-7
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DOI: https://doi.org/10.1007/s13205-017-1028-7