Abstract
Hydroxyapatite nanoparticles have been demonstrated as eminent nanomaterials because of their upgraded bioactivity, non-toxicity and biocompatibility properties. In this current examination, an upgraded wound healing activity of bioactive metabolites of medicinal polyherbal formulation Triphala fabricated hydroxyapatite nanocomposite was explored utilising in vivo excision wound Wistar rat model. Antibacterial activity of nanocomposite was studied against human pathogenic bacterial strains Pseudomonas aeruginosa and Staphylococcus aureus. Nanofabrication of the nanocomposite on wound dressing material and its antibacterial activity was additionally examined. Metabolites were extricated from Triphala utilising ethanol, and the extracted metabolites were fabricated with nano-hydroxyapatite utilising green science standards as profoundly stable hydroxyapatite nanocomposite. An ointment prepared from the synthesised nanocomposite was assessed for in vivo wound healing on excision wound created in a Wistar rat model. Nanocomposite treatment has shown complete wound-healing activity on the 21st day by recording all signs of the healing mechanism. Synthesised nanocomposite reveals growth inhibition of both the tested bacterial strains by showing zone of inhibition, biofilm inhibition. Antibacterial activity was also studied with nanocomposite, fabricated on dressing material which reveals the fabricated material inhibited the growth of the tested bacterial pathogenic strains. The current investigation proposes the possible use of phytonanotechnology standards as a successful course of wound mending and restraint of twisted disease without the help of manufactured antimicrobials.
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Thanks to Dr Hari Hara Sivakumar, Professor, Department of Pharmacology, KMCH College of Pharmacy, Coimbatore, and Tamil Nadu, for assisting in animal model studies.
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Animal model studies was followed by the rules of the Committee for Control and Supervision of Experiments on Rats (CPCSEA), and the convention was affirmed by the Institutional Animal Ethics Committee (IAEC No: KMCRET/B, TECH/01/2017-18).
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Namasivayam, S.K.R., Venkatachalam, G. & Bharani, R.S.A. Noteworthy enhancement of wound-healing activity of triphala biomass metabolite-loaded hydroxyapatite nanocomposite. Appl Nanosci 11, 1511–1530 (2021). https://doi.org/10.1007/s13204-021-01813-8
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DOI: https://doi.org/10.1007/s13204-021-01813-8