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Dual gold nanostructure-based electrochemical immunosensor for CA125 detection

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Abstract

With an aim to develop a sensitive immunoassay for ovarian cancer detection that has linearity over a broad range, we hereby report a dual gold nanostructure-based electrochemical immunosensor to detect ovarian cancer biomarker carcinoma antigen 125 (CA125) in serum. Gold nanorods (GNRs) are coated on indium tin oxide (ITO) and used as working electrode after immobilizing capture anti-CA125 on it. Second, gold nanoparticles (GNPs) tagged with cadmium ion (Cd2+) and another CA125 antibody is used as a probe for signal generation. A sandwich between gold nanorod-modified working electrode and GNP-based probe is formed through CA125 antigen. Direct detection of probe bound on the surface of immunosensor was done by differential pulse voltammetry (DPV) and the peak signals were directly proportional to the CA125 antigen concentration. The developed sensor has shown a detection limit of 3.4 U mL−1 with a broad linear detection range of 20–100 U mL−1. The immunosensor is stable, reproducible and has a good correlation coefficient of 0.97 for the clinical serum samples tested with the developed immunosensor and enzyme-linked immunosorbent assay (ELISA). It has the potential to detect CA125 in clinical samples which show abnormally high or low levels of CA125 in serum.

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Acknowledgements

We gratefully acknowledge Director of MNNIT Allahabad for providing necessary research facilities, Sophisticated Analysis Instrumentation Facility, Panjab University, Chandigarh for TEM facility, Centre for Interdisciplinary Research (CIR), MNNIT, Allahabad for electrochemical workstation facility and Design and Innovation Centre (DIC), Ministry of Human Resource and Development (MHRD), New Delhi for their kind support. The authors also thank sincerely to Dr. Manisha Sachan, Assistant Professor, Department of Biotechnology, MNNIT Allahabad and Kings George Medical University (KGMU, Lucknow, India) for their generous effort in providing serum samples of ovarian cancer patients.

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This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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  1. Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad, 211004, UP, India

    Nitin Kumar, Shivesh Sharma & Seema Nara

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  1. Nitin Kumar
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  2. Shivesh Sharma
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Correspondence to Seema Nara.

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Kumar, N., Sharma, S. & Nara, S. Dual gold nanostructure-based electrochemical immunosensor for CA125 detection. Appl Nanosci 8, 1843–1853 (2018). https://doi.org/10.1007/s13204-018-0857-y

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