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Aptasensor for ovarian cancer biomarker detection using nanostructured gold electrodes

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Abstract

The development of an electrochemical aptasensor for the detection of CA125 as an ovarian cancer biomarker using gold nanostructures (GNs) modified electrodes is reported. The GNs were deposited on the surface of fluorine-doped tin oxide electrodes using a simple electrochemical method and the effects of pH and surfactant concentration on the topography and electrochemical properties of the resulting GNs modified electrodes were investigated. The electrodes were characterized using field-emission scanning electron microscopy and X-ray diffraction, cyclic voltammetry, and electrochemical impedance spectroscopy. The best electrode, in terms of the uniformity of the deposited GNs and the increase in electroactive surface area, was used for development of an aptasensor for CA125 tumor marker detection in human serum. Signal amplification was done by using aptamer-conjugated gold nanorods resulting in the detection limit of 2.6 U/ml and a linear range of 10 to 800 U/ml. The results showed that without the need for expensive antibodies, the developed aptasensor could specifically measure the clinically relevant concentrations of the tumor marker in human serum.

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Acknowledgements

Tarbiat Modares University is acknowledged for partial financial support of this work.

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Authors and Affiliations

  1. Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, P.O. Box, Tehran, 14115-175, Iran

    Masood Amirabadizadeh & Maryam Nikkhah

  2. Department of Physics, Faculty of Basic Sciences, Tarbiat Modares University, P.O. Box, Tehran, 14115-175, Iran

    Hossein Siampour, Sara Abbasian & Ahmad Moshaii

  3. Biosensor Research Center (BRC), Isfahan University of Medical Sciences, P.O. Box, Isfahan, 81746-73461, Iran

    Hossein Siampour

  4. Department of Sensor and Biosensor, Faculty of Interdisciplinary Sciences and Technologies, Tarbiat Modares University, P.O. Box, Tehran, 14115-336, Iran

    Maryam Nikkhah & Ahmad Moshaii

Authors
  1. Masood Amirabadizadeh
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  2. Hossein Siampour
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  3. Sara Abbasian
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  4. Maryam Nikkhah
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Correspondence to Maryam Nikkhah.

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Amirabadizadeh, M., Siampour, H., Abbasian, S. et al. Aptasensor for ovarian cancer biomarker detection using nanostructured gold electrodes. Microchim Acta 191, 2 (2024). https://doi.org/10.1007/s00604-023-06072-8

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