Abstract
Hydrogels are water-insoluble crosslinked hydrophilic networks capable of retaining a large amount of water. The present work aimed to develop a novel chitosan–PVA-based hydrogel which could behave both as a nanoreactor and an immobilizing matrix for silver nanoparticles (AgNPs) with promising antibacterial applications. The hydrogel containing AgNPs were prepared by repeated freeze–thaw treatment using varying amounts of the crosslinker, followed by in situ reduction with sodium borohydride as a reducing agent. Characterization studies established that the hydrogel provides a controlled and uniform distribution of nanoparticles within the polymeric network without addition of any further stabilizer. The average particle size was found to be 13 nm with size distribution from 8 to 21 nm as per HR-TEM studies. Swelling studies confirmed that higher amount of crosslinker and silver incorporation inside the gel matrices significantly enhanced the porosity and chain entanglement of the polymeric species of the hydrogel, respectively. The AgNP-hydrogel exhibited good antibacterial activity and was found to cause significant reduction in microbial growth (Escherichia coli) in 12 h while such activity was not observed for the hydrogel without AgNPs.
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Introduction
Hydrogel is a water-insoluble gel-like polymeric network which swells in water due to extensive crosslinking. Its potential to retain significant amount of water is attributed to the presence of several functional groups (i.e., amino, carboxyl, amide, hydroxyl and sulphonic) in the polymers of the hydrogel forming network (Peppas and Khare 1993). Hydrogels usually have well-defined structures that can be modified to yield tailorable functionality and release profile (Mukherji et al. 2012). This has attracted many researchers toward its usage for antibacterial applications either by direct use of the polymer for its antibacterial property or, by incorporating antibacterial agents on the polymeric hydrogel network (Rabea et al. 2003). Chitosan, a natural biopolymer produced in bulk quantity from the shells of crustaceans as byproduct, can be chosen as a promising candidate for making antibacterial hydrogels owing to its innate antibacterial property (Shi et al. 2006). Other favorable properties such as gel forming capability, solubility in dilute acids, and low toxicity make it a suitable material for biomedical purposes. However, the main disadvantage associated with chitosan hydrogel is its poor mechanical strength during swelling which may limit its usage for water disinfection purposes. This can be minimized either by physical blending and/or chemical modification by grafting, interpenetrating polymer networks and crosslinking methods (Chandy and Sharma 1992). Chemical crosslinking of chitosan with a polymer having greater mechanical strength, good chemical stability, and hydrophilicity such as polyvinyl alcohol (PVA) may lead to fabrication of a hydrogel composite for antibacterial applications (Zhaoa et al. 2003; Xiao et al. 2010; Hang et al. 2010).
In recent years, there has been growing interest on the usage of silver nanoparticles (AgNPs) for numerous antimicrobial applications, such as, in surgical devices, wound dressings, water disinfection, and antibacterial coatings (Ruparelia et al. 2008; Chen and Schluesener 2008). It is now established that silver nanoparticles exhibit superior antibacterial property than its bulk form and is highly effective against bacteria, virus, and fungi (Li et al. 2010). Most microorganisms find it hard to develop resistance against AgNPs (Galdiero et al. 2011). However, there are some limitations associated with their use for disinfection purposes, when present in aqueous phase. For example, it is difficult to control the shape, size, as well as stability of silver nanoparticles in solution since they undergo rapid oxidation and tend to form aggregates. This may result in reduction of its antimicrobial efficacy at a particular concentration. Also, the cost associated with one time usage of silver nanoparticles and their potential environmental risk to aquatic ecosystem should be taken into consideration. In order to minimize the risk, one promising approach entails the incorporation of silver nanoparticles inside a polymeric matrix that ensures sufficient stabilization with minimum leaching of silver nanoparticles from it.
Till date, several polymeric materials for incorporation of silver nanoparticles have been investigated in the form of microparticles, multilayered films, nanofibers, and hydrogel structures (Zhang et al. 2004; Wu et al. 2009; Yuana et al. 2010; Ma et al. 2009). Although these hybrid materials provide good stability to the synthesized nanoparticles, sincere research efforts have been made to synthesize new hydrogel nanoparticulate system where both the hydrogel and nanoparticle could retain their individual features and characteristics in the combined system (Hamidi et al. 2008; Mohan et al. 2007). In their swollen stage, hydrogels offer large free space within the crosslinked network that can act as a nanoreactor and thus facilitate synthesis of nanoparticles by providing sufficient space for nucleation and growth of nanoparticles (Wang et al. 2004; Mohan et al. 2006, 2007). Inside the hydrogel matrix, silver ions make favorable interactions with the functional moieties present in the polymeric chain and thus, form a uniform distribution inside the whole network (Murthy et al. 2008; Basit et al. 2008). Upon reduction, uniformly distributed silver nanoparticles of controlled size may be achieved which would subsequently immobilize within the polymeric matrix. Recently, a few studies have shown the use of chitosan–PVA hydrogels for antimicrobial and food packaging applications (Tripathi et al. 2009; Hang et al. 2010; Vimala et al. 2011). However, no study has reported their dual role as template-mediated in situ formation and immobilization of silver nanoparticles into the hybrid polymer, for potable water disinfection purposes. Therefore, the aim of the current work is to synthesize and characterize the antimicrobial chitosan–PVA hydrogel which would serve both as a template and an immobilizing matrix for silver nanoparticles. Consequently, the combination of silver nanoparticles within chitosan–PVA polymeric architecture seems to be a promising candidate for the design of new antimicrobial materials.
Materials and methods
Chitosan (<90 % deacetylation degree, MW 100,000–300,000) was purchased from Marine Chemicals, Kochin, and was used as received. PVA (MW 85,000–124,000) was procured from SD Fine Ltd, Mumbai, and was used without purification. Silver nitrate was obtained from Merck India Pvt Ltd. All other chemicals, i.e., sodium hydroxide, glutaraldehyde solution (25 wt%), glacial acetic acid, and sodium borohydride were obtained from SD Fine Ltd and were of analytical grade. For antibacterial experiments, the E. coli MTCC443 strain (ATCC 25922) was procured from Institute of Microbial Technology (IMTECH-Chandigarh, India). Deionized water was used throughout the experiments and as polymerization medium during hydrogel formation.
Preparation of chitosan/PVA blended hydrogel
Chitosan polymer (2 % w/v) was dissolved in dilute acetic acid solution at room temperature while stirring overnight. The solution was filtered to remove any impurities and/or undissolved polymer before use. PVA solution (4 % w/v) was prepared by dissolving PVA in deionized water at 80 °C with stirring for 4 h. Synthesis of chitosan–PVA hydrogel was done in the following manner. A mixture of chitosan and PVA solution was stirred at 60 °C for 2 h to obtain a homogenous phase and left overnight with continuous stirring. To achieve network formation, glutaraldehyde was employed as a crosslinker and added to the resulting polymeric mixture. For this, the required amount of glutaraldehyde (25 and 50 mM, 1 ml) was added slowly to the chitosan–PVA solution at 25 °C and was mixed well for 5 min before casting the resultant solution in the petri dish. The hydrogel membranes were removed from the plate by precipitating in 12 wt% NaOH (aq) at room temperature for 1 h, washed thrice with distilled water, then soaked in distilled water for 24 h and lyophilized. Finally, porous semi-interpenetrating networks were developed by repeated freezing and thawing cycles at −20 °C for 6 h and 25 °C for 2 h, respectively, for the next 7 days. In this way, two types of chitosan (2 %)/PVA (4 %) hydrogel were prepared with different crosslinking densities achieved using 25 and 50 mM glutaraldehyde.
In situ synthesis and immobilization of silver nanoparticles inside the hydrogel network
Preparation of Ag containing chitosan–PVA hydrogel was performed as follows. Here, in situ reduction of silver ions inside the hydrogel network was done using sodium borohydride as reducing agent. Briefly, various circular discs (10 mm diameter) were cut from the prepared hydrogel membrane and were introduced in the 10 mM solution of silver nitrate (silver ion precursor) for 24 h. The silver ion-loaded discs were rinsed twice with deionized water in order to remove the unbound silver ions and were immersed in sodium borohydride solution (10 mM) for 30 min. After complete reduction, the discs were again rinsed using deionized water, lyophilized and kept for further characterization. The schematic representation of silver nanoparticle formation inside the hydrogel network is shown as below.
Swelling studies on the chitosan/PVA blended hydrogel membranes
The chitosan/PVA blended hydrogel membrane was cut into 10-mm circular discs and dried under vacuum for 72 h to determine their dry weight (W2). Water content was measured by immersing the chitosan/PVA blended hydrogel membrane in distilled water at 25 °C for 2 h. The wet weight (W1) was determined by wiping off the surface water with a piece of filter paper. The absorbed water content was then calculated by determining the water content in term of the swelling ratio (S %) using Eq. 1:
Characterization of hydrogel composites
The Fourier transform infrared (FTIR) spectra of pure chitosan–PVA and Ag immobilized Chitosan–PVA were recorded with an FTIR-Spectrophotometer (MAGNA 550, Nicolet Instruments Corporation, USA) using KBr pellet. UV–visible spectra of the silver nanoparticles from the nanocomposite hydrogel were recorded using a spectrophotometer (Perkin-Elmer, CA) in the absorbance mode (range 200–800 nm). The morphology of hydrogel membrane was observed by FEG-SEM (JEOL JSM-7600, Japan) with platinum coating using a sputter system. The particle size and size distribution of AgNPs in the composite hydrogel was determined using HR-TEM (JEOL JEM 2100F, Japan) at an operational voltage of 200 kV. Mass loading of AgNP inside the hydrogel was determined using inductively coupled plasma-atomic emission spectrometer (ARCOS ICP-AES spectro, Germany) using the following procedure. Initially, a pre-weighed hydrogel was crushed into powdered form and was immersed in 5 ml of deionized water overnight. The solution was then sonicated for 2 h, treated with nitric acid (70 %, 200 μl) to ensure dissolution of the AgNPs and finally filtered (Whatman 40) to remove the hydrogel debris. Various dilutions were tested using silver standards in the range of 10 ppb to 100 ppm. The presence of residual boron in the hydrogel was also investigated through ICP-AES. The study was conducted in duplicate.
Antibacterial activity of the hydrogel
The antibacterial activity of these hydrogel nanocomposites was determined using E. coli MTCC443 strain in nutrient broth medium. The sterilized medium was inoculated using 10 μl of microbial culture and kept in the rotary shaker at 150 rpm, 37 °C for 16 h. At the mid-log phase of microbial growth, cells were centrifuged, washed thrice and resuspended in 2 % phosphate buffer. The final cell concentration was adjusted to unit absorbance at 600 nm using UV spectrophotometer which corresponded to 1–2 × 109 CFU/ml. The antibacterial activity of the composite hydrogel was tested by immersing the hydrogel discs (10 mm diameter, 2 in number) into the microbial solution and incubating under aforementioned conditions. Samples were withdrawn from the test solution at different time intervals for determining the biocidal action of the composite hydrogel. Pure chitosan–PVA hydrogel was also used as a control for the antibacterial study. The growth kinetics of pure culture solution (devoid of hydrogel and silver nanoparticles) was also employed as a negative control.
Results and discussion
Template-mediated in situ formation of silver nanoparticles in the hydrogel
Due to the importance of chitosan–silver nanofilms/membranes in antimicrobial application, water purification and wound dressing applications, a chitosan/PVA hydrogel was developed to act both as a nanoreactor as well as a template for immobilization of silver nanoparticles. The hydrogel networks acted as a carrier in the reaction process and subsequently behaved as an immobilizing matrix for the synthesized nanoparticles (Mohan et al. 2007). An uniform distribution of silver nanoparticles inside the hydrogel could be achieved due to its tendency to form complex and/or non-covalent interactions with silver ions and the functional moieties present in the hydrogel network such as –OH, –NH2, –C=O (Tarnavchyk et al. 2009; Bozanic et al. 2010; Mohan et al. 2010). The addition of the fully swollen hydrogel to silver nitrate solution caused adsorption/anchoring of Ag+ ions with the functional groups via ion-exchange mechanism. Also, many silver ions may get trapped in the free spaces between the crosslinked polymeric networks (Mohan et al. 2010). Thus, reduction of silver ions using any reducing agent results in the formation of silver nanoparticles within as well as in the free spaces between the polymeric chains. In the present approach, silver-loaded hydrogel networks were reduced in situ with NaBH4 to form highly dispersed silver nanoparticles. Since sodium borohydride is a strong reducing agent, the color of the hydrogel changed to deep yellow within 10 s which indicated the formation of silver nuclei. However, color of the discs changed gradually from yellow to light brown and finally to deep brown over time (30 min) which indicated the formation of silver nanoparticles, as shown in Fig. 1.
It is reported that, change in concentration of the crosslinking agent may affect the free space between the polymeric networks, and may affect the size, shape or distribution of the silver nanoparticles within the hydrogel. This can be achieved either by altering the crosslinker amount or by choosing different types of crosslinker, often in different combinations. A highly dense hydrogel network is desired which favors the intra–inter molecular interactions between the silver ions and the gel network, resulting in the formation of smaller size silver nanoparticles. In contrast, a hydrogel with high porosity provides large surface area for silver nuclei generation and thus, produces a large number of silver nanoparticles with its potential to limit their aggregation. The porosity affords an opportunity for the rapid diffusion of water molecules into and out of the polymers and this property may be important for antibacterial applications. Therefore, the crosslinker density appears to be a key factor for in situ formation and immobilization of silver nanoparticles inside the hydrogel.
Considering the toxic effects of glutaraldehyde, a minimum amount of crosslinker (25 and 50 mM) was used in forming the hydrogels. In a study by Spencer et al. (1978), male rats when exposed to 0.25 % glutaraldehyde (i.e., 25 mM) in their drinking water for 11 weeks exhibited no evidence of toxicity or damage to the peripheral or central nervous systems. Several studies have reported effective removal of unused glutaraldehyde by repeated washing with deionized water (Hoffmann et al. 2009). Moreover, in some established protocols for microscopic fixation, unreacted glutaraldehyde is completely removed by treatment with sodium borohydride (Gard and Kropf 1993). In the current study, the synthesized hydrogels were extensively washed with DI water to remove the unreacted glutaraldehyde. Subsequently, an excess of sodium borohydride was used during in situ AgNP formation. This would further ensure complete removal of glutaraldehyde from the hydrogel. Thus, the chitosan–PVA hydrogels synthesized using two different concentrations of glutaraldehyde, i.e., 25 and 50 mM (designated as CP-25 and CP-50, respectively) are not expected to contain any unreacted glutaraldehyde that may contribute to toxicity.
Swelling studies
The impact of silver nanoparticle incorporation on the chitosan–PVA hydrogel and crosslinking densities on the swelling behavior was evaluated as shown in Table 1. The results showed that for 25 and 50 mM crosslinker concentration, pure hydrogel had swelling ratios of 11 and 26 %, respectively, while for Ag-loaded hydrogel it decreased to 5 and 22 %, respectively. This can be explained on the basis of additional cross-links between AgNPs and the electron-rich O and N atoms present in the polymeric chains which restrained the water penetration ability of the hydrogel and decreased the overall swelling behavior (Vimala et al. 2011). In another study, Ma et al. (2009) established that formation of silver nanoparticles inside the polymeric matrix enhanced the flexibility of the hydrogel network due to stronger interactions between Ag and the hydrophilic moieties present in the polymer. The resulting decrease in hydrophilicity of the composite hydrogel reduced the swelling behavior. Swelling studies also revealed that a higher concentration of the crosslinker could significantly increase the porosity and template area for nanoparticle formation, resulting in an enhanced swelling ratio. Wang and Gunasekaran (2006) studied the effect of crosslinker amount on the swelling ratio of the hydrogel and postulated that higher crosslinker amount leads to higher degree of localization of water molecules inside the hydrogel network resulting in an increase in the swelling ratio. This can be explained on the basis of stronger interactions and flexible chain entanglements between the two polymers blended as the crosslinker amount is increased.
Figure 2 shows the swelling kinetics of chitosan hydrogels in deionized water (pH 7) at room temperature. For both CP-25 and CP-50, there was a gradual increase in the swelling ratio up to 120 min after which no significant change was observed. Swelling ratio at this stage was termed as the equilibrium swelling ratio, whose value was 11.63 ± 0.18 and 25.85 ± 0.38 % for CP-25 and CP-50 hydrogel, respectively. However, it should be noted that the equilibrium swelling ratio is a temperature-dependent property which varies with formation or breakup of hydrogen bonds between hydroxyl groups of PVA and amino groups present within the chitosan polymeric network (Kim et al. 2003).
On the basis of higher hydrophilic nature, CP-50 hydrogel was chosen over CP-25 for further characterizations and antimicrobial study. Chung et al. (2004) illustrated a correlation between hydrophilic nature of the chitosan and its antibacterial activity against Gram negative bacteria, i.e., E. coli.
Characterization of synthesized chitosan–PVA hydrogel
FTIR studies
The FTIR spectrum of synthesized chitosan–PVA hydrogel (CP-50) and nano-silver-loaded hydrogel was evaluated (Fig. 3). Result showed that the synthesized hydrogels displayed good compatibility between chitosan and PVA polymer with significant changes observed after silver nanoparticle incorporation. A peak appeared at 3,445 cm−1 indicating the presence of hydrogen bonds between chitosan and PVA polymers causing OH/NH2 stretching (Vimala et al. 2011). Other peaks, i.e., 1,721, 1,635 and 1,418 cm−1 may be attributed to carbonyl group stretching, absorption of NHCOCH3 groups and C–H bonds present, respectively, in the chitosan/PVA polymeric chains (Lin et al. 2011; Yang et al. 2004; Vimala et al. 2011). The appearance of carbonyl group in chitosan/PVA hydrogel, even after crosslinking with glutaraldehyde indicates free –OH moieties of PVA due to the incomplete reaction of glutaraldehyde with hydroxyl groups of PVA (Mansur et al. 2008). These hydroxyl groups therefore represent ideal sites for the anchoring of silver ions, contributing later to the silver nanoparticle immobilization. Interestingly, the absorption peaks were observed at 2,955 and 2,860 cm−1 in both pristine and the nano-silver-loaded hydrogels, which originate from CH2 asymmetric stretching and C–H bond of chitosan/PVA and chitosan chains, respectively (Xiao et al. 2010; Vimala et al. 2011). However, their intensity was increased in nano-silver-loaded hydrogel, which confirmed that the incorporation of silver nanoparticles could enhance stretching of the polymeric backbone chain, resulting in an increase in the flexibility of the hydrogel, as also demonstrated by the swelling studies.
UV–vis spectroscopy
Synthesis of silver nanoparticles inside the hydrogel networks was analyzed by UV-spectroscopy as shown in Fig. 4. The composite hydrogel showed an absorption peak at 428 nm, which is due to the localized surface plasmon resonance (LSPR) of silver nanoparticles. The spectra of the hydrogel film showed a relatively broad band which was rather unexpected. It could be indicative of a distribution of silver nanoparticles with larger particle size range. Another probable reason could be the immobilization of silver nanoparticle deep within the hydrogel film which may cause their spectral behavior to differ from those of isolated nanoparticles. To overcome the possibility of hindrance within the immobilizing network, silver nanoparticles were retrieved from the hydrogel structure as described by Thomas et al. (2009). AgNP containing hydrogel films (CP-50/nano-Ag) were sonicated in deionized water so as to facilitate the relaxation of polymeric chain entanglement within the hydrogel network. As a result, mesh size of the swollen gel increased, ensuring recovery of some of the silver nanoparticles in colloidal form. Interestingly, these nanoparticles displayed a sharp peak at 400 nm which confirmed the presence of small-sized nanoparticles with a narrow size distribution. This blue shift of 28 nm in the absorption maxima indicated that the actual size of silver nanoparticles would be even smaller than that expected through the UV–vis study. Thus, HR-TEM analysis was performed further to identify the exact size and distribution of the silver nanoparticles synthesized inside the hydrogel films. However, in both spectra no absorption peak was observed at 335 and 560 nm which indicated the absence of any AgNP aggregates and silver clusters within the hydrogel (Gutiérrez and Henglein 1993; Ma et al. 2009).
HR-TEM studies
HR-TEM study provides valuable information regarding size and distribution of AgNP within hydrogel nanocomposites. Figure 5 indicated that the average size of AgNP was 13.3 ± 3.8 nm with size distribution in the range of 8–21 nm (inset). No aggregates of nanoparticles were observed.
FEG-SEM studies
The cross-sectional morphology of the hydrogels was observed to evaluate in situ formation of silver nanoparticles inside the hydrogel networks. As shown in Fig. 6a, b, pristine hydrogel (CP-50) did not show any particles in the FEG-SEM image. Loading of silver nanoparticles inside the hydrogel did not affect its morphology and structural integrity (Fig. 6c, d). Figure 6e, f showed that silver nanoparticles were evenly distributed between the polymeric chain networks with a size range of 25–35 nm. This corroborates with the UV absorption maxima at 428 nm which corresponds to silver nanoparticles of similar size range as shown by Hutter and Fendler (2004). However, it is not possible to judge the actual size of impregnated silver nanoparticles inside the hydrogels from FEG-SEM results where analysis is performed on dried hydrogel samples as discussed by Mohan et al. (2010). Thus, the size of silver nanoparticles determined using HR-TEM studies, i.e., 13.3 nm was considered to be more appropriate for this study. As shown by the FEG-SEM results, the main advantage of the current technique is that it provided a controlled and uniform distribution of nanoparticles within the hydrogel network without addition of any other stabilizer. It was expected that a homogeneous distribution of silver nanoparticles would be achieved throughout the hydrogel networks, although there was a rapid reduction of the silver ions into the silver nanoparticles with sodium borohydride. The reason is that the silver nanoparticles initially formed in the network causes expansion of the free space in the hydrogel, since silver ions interact electrostatically with hydroxyl groups of the PVA moieties. This made it possible for NaBH4 to enter into the entire gel network to reduce the silver ions present in the network. Nevertheless, the presence of intra- and inter-molecular forces between chitosan and polyvinyl alcohol units facilitated formation of an extensive physical network of hydrogen bonds, which provided ‘nano’ domains for growth of the nanoparticles as well as ensured their protection within the network. Furthermore, chitosan chains in the hydrogel possibly participated in the reduction process, as also suggested by previous studies (Vimala et al. 2011).
Amount of silver in the hydrogel
The amount of silver nanoparticles loaded inside the hydrogel was evaluated using ICP-AES. Crushing of the hydrogel and sonication were used to completely retrieve AgNPs from the interior of the hydrogel matrix while nitric acid digestion was used to ensure dissolution of the AgNPs. The solution obtained after extracting and dissolving the AgNPs loaded on a hydrogel (0.218 g) in 5 ml acidified deionized water contained 22.3 ± 2.3 ppm of silver and 19.2 ± 4.7 ppb of boron based on ICP studies. Thus, the CP-50 hydrogel contained 0.51 ± 0.06 mg of AgNP/g of CP-50 hydrogel (0.05 % AgNP). This confirms significant immobilization of AgNPs within the hydrogel network. Boron constituted less than 0.1 % of the mass of silver nanoparticles immobilized inside the hydrogel. Thus, traces of boron impurity exist and it cannot be completely excluded as also reported in other studies (Ruparelia et al. 2008).
Antibacterial studies
The antibacterial action of synthesized hydrogel (CP-50) is an important factor for determining its application in water disinfection devices. Here, the pure hydrogel and nano-Ag-loaded hydrogels tested against E. coli MTCC443 strain showed a significant reduction in the bacterial growth over an incubation period of 720 min (Fig. 7). After 12 h, there was 83.5 % reduction in E. coli growth for Ag-incorporated hydrogel compared to 24.4 % reduction for the pure hydrogel. Moreover, it was found that silver-immobilized hydrogels showed superior antimicrobial efficacy compared to its pure form at all sampling times. The presence of the porous hydrogel facilitates frequent diffusion of liquid media across the hydrogel which could release silver nanoparticles into it (Vimala et al. 2011). However, the basis for growth inhibition in pure chitosan–PVA was due to the inherent antimicrobial nature of the chitosan polymer present in the hybrid network (Shi et al. 2006). Polycationic chitosan can interact with the negatively charged moieties present on the bacterial surface causing membrane leakage, loss in cell permeability and cell death. Therefore, porous hydrogel–silver nanocomposites exhibited good antimicrobial activity and displayed enhanced biocidal efficacy for disinfection purposes.
Conclusion
A porous, chemically crosslinked chitosan–PVA hydrogel was prepared by repeated freeze–thaw treatment for promising antibacterial applications. The hydrogel is an ideal candidate for the successful in situ synthesis of silver nanoparticles within the polymeric networks, which also serve as an immobilizing matrix. Morphology studies (FEG-SEM, FEG-TEM) indicate that AgNPs are located on the surface as well as interior of the hydrogel and an effective incorporation of metal nanoparticles into the hydrogel network was achieved. An additional advantage is that a uniform distribution of silver nanoparticles with narrow size distribution was achieved without use of any stabilizer. Swelling studies also revealed that a higher crosslinker concentration and incorporation of silver nanoparticles enhanced the overall morphology of the hydrogel structure, i.e., they imparted higher porosity and flexibility to the polymeric chain units. The developed nanocomposite hydrogel showed effective biocidal action against E. coli within 12 h, thus showing great potential for use in water disinfection purposes.
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Acknowledgments
The authors would like to acknowledge use of research facilities at Centre for Research in NanoTechnology and Sciences (CRNTS), and Sophisticated Analytical Instrumentation Facility (SAIF), IIT-Bombay. The antimicrobial experiments were conducted at the Centre for Environmental Science and Engineering (CESE), IIT-Bombay. Financial support from the DST-funded project [Centre for Nanotechnology (Polymer nanosensors, Nanobiotechnology, and Nanoelectronics)] is greatly acknowledged.
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Agnihotri, S., Mukherji, S. & Mukherji, S. Antimicrobial chitosan–PVA hydrogel as a nanoreactor and immobilizing matrix for silver nanoparticles. Appl Nanosci 2, 179–188 (2012). https://doi.org/10.1007/s13204-012-0080-1
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DOI: https://doi.org/10.1007/s13204-012-0080-1