Abstract
Delftia sp. strain JD2 is a soil bacterium that produces auxins and acts as a nodulation-assisting bacterium in legumes inoculated with rhizobia. The co-inoculation of soybean (Glycine max) seeds with Bradyrhizobium elkanii and JD2 enhances plant growth, nitrogen fixation, and grain yield. This work aimed to characterize bioactive compounds in soybean plants under different inoculation treatments. Metabolomic profiling by Ultra-High-Performance Liquid Chromatography coupled with High-Resolution Mass Spectrometry (UHPLC-HRMS) was carried out on freeze-dried rhizospheric exudates and root extracts from hydroponic or greenhouse plants experiments, respectively. The differential metabolomic profiling between treatments was analyzed by pair-wise comparisons. Statistical results showed that both root and rhizospheric samples clustered according to the inoculation treatment and the sampling time of plants. This classification derives from the different production of cinnamic acids, coumarins, benzoic acids, and flavonoids, among other discriminant compounds between treatments. We observed the over-production of nod genes inducers in roots from plants inoculated with JD2. In contrast, a reduction in the discriminant flavonoids occurred in co-inoculated plants. JD2 positively influences the production of auxins. These changes in the composition of flavonoids and auxins are likely to explain the differences observed in the growth and nodulation of co-inoculated plants. This study emphasizes some metabolic changes that positively affect the co-inoculation of soybean seeds with bradyrhizobia and JD2.
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Riviezzi, B., Campmajó, G., Cagide, C. et al. Comparative metabolite analysis of Delftia-Bradyrhizobium co-inoculated soybean plants using UHPLC-HRMS-based metabolomic profiling. Symbiosis 85, 325–341 (2021). https://doi.org/10.1007/s13199-021-00818-9
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DOI: https://doi.org/10.1007/s13199-021-00818-9