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Identification of interior salt-tolerant bacteria from ice plant Mesembryanthemum crystallinum and evaluation of their promoting effects

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Abstract

Endophytic bacteria can stimulate host plant development. Insufficient information is available about NaCl-tolerant bacteria that colonize ice plants (Mesembryanthemum crystallinum) in their habitats. In this study, a culture-dependent method was used to isolate endophytic nitrogen-fixing bacteria from ice plants, and the resulting cultures were screened for salt-stress tolerance in vitro. A total of 17 salt-tolerant bacteria were obtained. The majority of the isolates grew well in 2.05 M NaCl with a maximum tolerance at 3.59 M. Most of the strains were Gram-positive bacteria with various plant growth-promoting traits. The 16S rRNA gene sequences revealed that the 17 isolates were distributed within three genera and corresponded to the bacterial species Halomonas sp., Bacillus sp., and Planococcus sp. Inoculation of cabbage (Brassica olereacea) seeds with selected strains showed that the strain MC1 promoted seed germination, and the same strain significantly increased root dry weight under saline stress by 24.5%. Our study suggests that ice plants naturally accommodate a variety of salt-tolerant endophytic bacteria and that these bacteria are able to relieve abiotic stress during plant growth.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (Grant No.31701968), the Anhui Science and Technology Project (1704a07020078), the Dean’s Youth Innovation Project (17B0307), and the Vegetable Cultivation Scientific Innovation Team (18C0309) from Anhui Academy of Agricultural Sciences. We gratefully acknowledge two anonymous referees and editors who provided valuable and constructive suggestions to improve this manuscript.

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Authors and Affiliations

  1. Institute of Horticulture, Anhui Academy of Agricultural Sciences, Nongke South Road 40#, Hefei, 230031, Anhui Province, China

    Jian Zhang, Pengcheng Wang, Hongmei Tian, Haikun Jiang, Yan Wang & Congsheng Yan

  2. Key Laboratory of Genetic Improvement and Ecophysiology of Horticultural Crops, Hefei, 230031, Anhui Province, China

    Jian Zhang, Pengcheng Wang, Hongmei Tian, Haikun Jiang, Yan Wang & Congsheng Yan

  3. School of Horticulture, Anhui Agricultural University, Hefei, 230036, Anhui Province, China

    Haikun Jiang

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  1. Jian Zhang
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  2. Pengcheng Wang
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  3. Hongmei Tian
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Contributions

ZJ and WPC planed and designed the research. ZJ, WPC, WY, JHK, and YCS performed the experiments. ZJ, WPC, and THM analyzed the data. ZJ, WPC, WY, and THM wrote the manuscript.

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Correspondence to Jian Zhang.

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Zhang, J., Wang, P., Tian, H. et al. Identification of interior salt-tolerant bacteria from ice plant Mesembryanthemum crystallinum and evaluation of their promoting effects. Symbiosis 76, 243–252 (2018). https://doi.org/10.1007/s13199-018-0551-6

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