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Isolation of Endophytic Plant Growth-Promoting Bacteria Associated with the Halophyte Salicornia europaea and Evaluation of their Promoting Activity Under Salt Stress

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Abstract

Several reports have highlighted that many plant growth-promoting endophytic bacteria (PGPE) can assist their host plants in coping with various biotic and abiotic stresses. However, information about the PGPE colonizing in the halophytes is still scarce. This study was designed to isolate and characterize PGPE from salt-accumulating halophyte Salicornia europaea grown under extreme salinity and to evaluate in vitro the bacterial mechanisms related to plant growth promotion. A total of 105 isolates were obtained from the surface-sterilized roots, stems, and assimilation twigs of S. europaea. Thirty-two isolates were initially selected for their ability to produce 1-aminocyclopropane-1-carboxylate deaminase as well as other properties such as production of indole-3-acetic acid and phosphate-solubilizing activities. The 16S rRNA gene-sequencing analysis revealed that these isolates belong to 13 different genera and 19 bacterial species. For these 32 strains, seed germination and seedling growth in axenically grown S. europaea seedlings at different NaCl concentrations (50–500 mM) were quantified. Five isolates possessing significant stimulation of the host plant growth were obtained. The five isolates were identified as Bacillus endophyticus, Bacillus tequilensis, Planococcus rifietoensis, Variovorax paradoxus, and Arthrobacter agilis. All the five strains could colonize and can be reisolated from the host plant interior tissues. These results demonstrate that habitat-adapted PGPE isolated from halophyte could enhance plant growth under saline stress conditions.

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Acknowledgments

This work was supported by the Foundation of Chinese Academy of Sciences (Nos. 2015-XBQN-B-16, TSS-2015-014-FW-3-1, KFJ-SW-STS-141 and Y371162) and the Foundation of Science & Technology Department of Xinjiang Uygur Autonomous Region (QN2015BS017), and by the National Natural Science Foundation of China (No. 31300432).

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Authors and Affiliations

  1. State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, China

    Shuai Zhao, Na Zhou, Zheng-Yong Zhao, Ke Zhang & Chang-Yan Tian

  2. University of Chinese Academy of Sciences, Beijing, China

    Na Zhou

  3. Shizuishan Seed Management Station, Shizuishan, China

    Guo-Hua Wu

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  1. Shuai Zhao
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  2. Na Zhou
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Correspondence to Chang-Yan Tian.

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Zhao, S., Zhou, N., Zhao, ZY. et al. Isolation of Endophytic Plant Growth-Promoting Bacteria Associated with the Halophyte Salicornia europaea and Evaluation of their Promoting Activity Under Salt Stress. Curr Microbiol 73, 574–581 (2016). https://doi.org/10.1007/s00284-016-1096-7

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  • DOI: https://doi.org/10.1007/s00284-016-1096-7

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