Skip to main content
Log in

Fractionation and identification of salty peptides from yeast extract

  • Short Communication
  • Published:
Journal of Food Science and Technology Aims and scope Submit manuscript

Abstract

Salty taste is an important sensory attribute in many foods, which stimulates the appetite. But high-salt diets bring many health risks, and salty alternatives should be explored to solve this problem. The salt-reducing agents may impart new odors in food. Therefore, the research should focus on developing a novel agent, which would replace the salt without affecting the taste of the food. Generally, some yeast extracts taste salty and can be used for replacing salts in foods without imparting any additional odor. In this study, we fractionated salty peptides from FA31 (Angel Yeast) by ultrafiltration, gel permeation chromatography, preparative liquid chromatography (pre-HPLC), with the combination of sensory evaluation, and the peptide sequence was identified by ESI-Q-TOF LC/MS as Asp-Asp, Glu-Asp, Asp-Asp-Asp, Ser-Pro-Glu, and Phe-Ile.

Graphic abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  • Alim A, Yang C, Song H, Liu Y, Zou T, Zhang Y, Zhang S (2019) The behavior of umami components in thermally treated yeast extract. Food Res Int 120:534–543

    Article  CAS  Google Scholar 

  • Brandsma I (2006a) Reducing sodium: a European perspective. Food Technol 60(3):25–29

    Google Scholar 

  • Brandsma I (2006b) Reducing sodium: a European perspective[J]. Food Technol 60(3):25–29

    Google Scholar 

  • Bruni R, Gianfranceschi G, Koch G (2005) On peptide de novo sequencing: a new approach. J Pept Sci 11:225–23410

    Article  CAS  Google Scholar 

  • Chandrashekar J, Kuhn C, Oka Y, Yarmolinsky DA, Hummler E, Ryba NJ, Zuker CS (2010) The cells and peripheral representation of sodium taste in mice. Nature 464:297–3015

    Article  CAS  Google Scholar 

  • Charve J, Manganiello S, Glabasnia A (2018) Analysis of umami taste compounds in a fermented corn sauce by means of sensory-guided fractionation. J Agric Food Chem 66:1863–1871

    Article  CAS  Google Scholar 

  • Ganesan B, Brown K, Irish DA, Brothersen C, McMahon DJ (2014) Manufacture and sensory analysis of reduced-and low-sodium cheddar and mozzarella cheeses. J Dairy Sci 97:1970–1982

    Article  CAS  Google Scholar 

  • Gębski J, Jezewska-Zychowicz M, Szlachciuk J et al (2019) Impact of nutritional claims on consumer preferences for bread with varied fiber and salt content. Food Qual Prefer 76:91–99

    Article  Google Scholar 

  • Inguglia ES, Zhang Z, Tiwari BK, Kerry JP, Burgess CM (2017) Salt reduction strategies in processed meat products—a review. Trends Food Sci Technol 59:70–78

    Article  CAS  Google Scholar 

  • Israr T, Rakha A, Sohail M, Rashid S, Shehzad A (2016) Salt reduction in baked products: strategies and constraints. Trends Food Sci Technol 51:98–105

    Article  CAS  Google Scholar 

  • Kang L (2017) Identification of Maillard Reactive peptides in beef and deducing the mechanism of its flavor products formation. Master Thesis. Beijing Technology and Business University

  • Li YN, Liu WY, Zhang SL, Cheng XY (2016) Separation, purification and analysis of salty peptides derived from bovine bone by chromatography and mass spectrometry. Meat Res 30(3):25–284

    CAS  Google Scholar 

  • Li YN, Liu WY, Zhang SL, Qiao XL, Chen WH, Qu C, Cheng XY (2016) Amino acid composition and stability analysis of salty peptides derived from bovine bone under simulated processing conditions. Meat Res 30(1):11–144

    CAS  Google Scholar 

  • Liem DG, Miremadi F, Keast RS (2011) Reducing sodium in foods: the effect on flavor. Nutrients 3:694–711

    Article  CAS  Google Scholar 

  • Liu J (2015) The mechanism of chicken peptide in flavor generation via Maillard reaction. Master Thesis. Beijing Technology and Business University

  • Liu J, Song H, Liu Y, Li P, Yao J, Xiong J (2015) Discovery of kokumi peptide from yeast extract by LC-Q-TOF-MS/MS and sensomics approach. J Sci Food Agric 95:3183–3194

    Article  CAS  Google Scholar 

  • Makoto Tada ISAH (1984) L-ornithyltaurineOrnithyltaurine, a new salty peptide. J Agrie Food Chem 32:992–996

    Article  Google Scholar 

  • Mitchell M, Brunton NP, Wilkinson MG (2011) Current salt reduction strategies and their effect on sensory acceptability: a study with reduced salt ready-meals. Eur Food Res Technol 232:529–539

    Article  CAS  Google Scholar 

  • Münch P, Hofmann T, Schieberle P (1997) Comparison of key odorants generated by thermal treatment of commercial and self-prepared yeast extracts: influence of the amino acid composition on odorant formation. J Agric Food Chem 45(4):1338–1344

    Article  Google Scholar 

  • Onuma T, Maruyama H, Sakai N (2018) Enhancement of saltiness perception by monosodium glutamate taste and soy sauce odor: a near infrared spectroscopy study. Chem Senses 43(3):151–167

    Article  Google Scholar 

  • Peng ZQ, Zhang YW, Guo XY (2011) Polypeptide common salt substitute and preparation method. CN102224921A

  • Silva HLA, Balthazar CF, Esmerino EA, Vieira AH, Cappato LP, Neto RPC, Verruck S, Cavalcanti RN, Portela JB, Andrade MM, Moraes J, Franco RM, Tavares MIB, Prudencio ES, Freitas MQ, Nascimento JS, Silva MC, Raices RSL, Cruz AG (2017) Effect of sodium reduction and flavor enhancer addition on probiotic prato cheese processing. Food Res Int 99:247–255

    Article  CAS  Google Scholar 

  • Steen H, Mann M (2004) The ABC’s (and XYZ’s) of peptide sequencing. Nat Rev Mol Cell Biol 5(9):699–711

    Article  CAS  Google Scholar 

  • Strazzullo P, D'Elia L, Ngianga-Bakwin K, Cappucccio FP (2009) Salt intake, stroke and cardiovascular disease: metaanalysis of prospective studies. BMJ 339:b4567

    Article  Google Scholar 

  • Van Der Klaauw NJ, Smith DV (1995) Taste quality profiles for fifteen organic and inorganic salts. Physiol Behav 58(2):295–306

    Article  Google Scholar 

  • Xu X, You M, Song H, Gong L, Pan W (2018) Investigation of umami and kokumi taste-active components in bovine bone marrow extract produced during enzymatic hydrolysis and Maillard reaction. Int J Food Sci Technol 53:2465–2481

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Huanlu Song.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 15 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zheng, Y., Tang, L., Yu, M. et al. Fractionation and identification of salty peptides from yeast extract. J Food Sci Technol 58, 1199–1208 (2021). https://doi.org/10.1007/s13197-020-04836-1

Download citation

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13197-020-04836-1

Keywords

Navigation