Skip to main content
Log in

Bio-preservative effect of blends of essential oils: natural anti-oxidant and anti-microbial agents for the shelf life enhancement of emulsion based chicken sausages

  • Original Article
  • Published:
Journal of Food Science and Technology Aims and scope Submit manuscript

Abstract

This work explores the efficacy and potential of four different blends of essential oils as bio-preservative for enhancement of shelf life of emulsion based ready-to-eat chicken sausages. Pre-optimized levels of four different blends of essential oils: 0.25% each of B-1, B-2, B-3 and 0.125% of B-4, were tried in the chicken sausages. Four different treatments along with control were then aerobically packaged and stored under refrigerated (4 ± 1 °C) conditions. An increase in pH and TBARS value was found to be significantly lower in the case of B-2 products. Significantly higher values for DPPH activity (% inhibition) and total phenolic content (µg/g) were also observed for B-2 products which indicated that B-2 products had better oxidative stability. Further, B-1 and B-2 products were observed with significantly lower microbial count; however, B-4 products received slightly higher sensory scores than B-2. It was found that B-1, 2 and 3 (each at 0.25%) and B-4 (0.125%) enhanced the shelf life of chicken sausages by 13–14 days, 16–17 days, 10–11 days and 6–7 days, respectively under refrigerated (4 ± 1 °C) storage.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Adiani V, Gupta S, Chatterjee S, Variyar PS, Sharma A (2015) Activity guided characterization of antioxidant components from essential oil of Nutmeg (Myristica fragrans). J Food Sci Technol 52(1):221–230

    CAS  Google Scholar 

  • Aggarwal SG, Goyal S (2012) Comparative analysis of antimicrobial activity of essential oil of Ocimum kilimandscharium. Asian J Pharma Clinical Res 5(1):53–55

    Google Scholar 

  • Alfonzo A, Martorana A, Guarrasi V, Barbera M, Gaglio R, Santulli A, Settanni L, Galati A, Moschetti G, Francesca N (2017) Effect of the lemon essential oils on the safety and sensory quality of salted sardines (Sardina pilchardus Walbaum). Food Cont 73:1265–1274

    CAS  Google Scholar 

  • AOAC (2005) Official Method of Analysis, 16th edn. Association of officical analytical chemists, Washington, DC

    Google Scholar 

  • APHA 2005 Ameruican Public Health Association. Compendium of methods for microbiological examination of foods. 4th Edition. Sperk M.L. Washington D.C

  • Badee A, Moawad R, ElNoketi M, Gouda M (2013) Antioxidant and antimicrobial activities of marjoram (Origanum majorana L.) essential oil. J. Appl Sci Res 9:1193–1201

    CAS  Google Scholar 

  • Chandran J, Nayana N, Roshini N, Nisha P (2017) Oxidative stability, thermal stability and acceptability of coconut oil flavored with essential oils from black pepper and ginger. J Food Sci Technol 54(1):144–152

    CAS  PubMed  Google Scholar 

  • Cooner DE, Beuchat LR (1984) Effects of essential oils from plants on growth of food spoilage yeasts. J Food Sci 49(2):429–434

    Google Scholar 

  • Cremer M, Chipley J (1977) Satellite food service system, time and temperature and microbiological and sensory quality of precooked frozen hamburger patties. J Food Protect 40:603–607

    CAS  Google Scholar 

  • Duncan DB (1955) Multiple range and multiple F tests. Biometrics 11:1–42

    Google Scholar 

  • El-Mesallamy AMD, El-Gerby M, Mohamed HM, El-Azim A, Awad A (2012) Antioxidant, antimicrobial, activities and volatile constituents of clove flower buds. J Essen Oil Bear Plants 15(6):900–907

    Google Scholar 

  • El-Sayed HS, Chizzola R, Ramadan AA, Edris AE (2017) Chemical composition and antimicrobial activity of garlic essential oils evaluated in organic solvent, emulsifying, and self-micro-emulsifying water based delivery systems. Food Chem 221:196–204

    CAS  PubMed  Google Scholar 

  • FAO 2017 http://www.fao.org/docrep/003/x6556e/x6556e04.htm Accessed on 12.12.2017

  • Han F, Ma G, Yang M, Yan L, Xiong W, Shu XuH (2017) Chemical composition and antioxidant activities of essential oils from different parts of the oregano. J Zhejiang Univ Sci B 18(1):79–84

    CAS  PubMed  PubMed Central  Google Scholar 

  • Hassanien MFR, Assiri AMA, Alzohairy AM, Oraby HF (2015) Health-promoting value and food applications of black cumin essential oil: an overview. J Food Sci Technol 52(10):6136–6142

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ibrahim H, Moawad R, Emam W (2012) Antioxidant effect of pomegranate rind, seed extracts and pomegranate juice on lipid oxidation and some quality properties of cooked beef patties. J Appl Sci Res 8:4023–4032

    Google Scholar 

  • Jalali N, Ariiai P, Fattahi E (2016) Effect of alginate/carboxyl methyl cellulose composite coating incorporated with clove essential oil on the quality of silver carp fillet and Escherichia coli O157:H7 inhibition during refrigerated storage. J Food Sci Technol 53(1):757–765

    CAS  PubMed  Google Scholar 

  • Karabagias I, Badeka A, Kontominas MG (2011) Shelf life extension of lamb meat using thyme or oregano essential oils and modified atmosphere packaging. Meat Sci 88:109–116

    CAS  PubMed  Google Scholar 

  • Keeton JT (1983) Effects of fat and NaCl/phosphate levels on the chemical and sensory properties of pork patties. J Food Sci 48:878–881

    CAS  Google Scholar 

  • Khaleque MA, Keya CA, Hasan KN, Hoque MM, Inatsu Y, Bari ML (2016) Use of cloves and cinnamon essential oil to inactivate Listeria monocytogenes in ground beef at freezing and refrigeration temperatures. LWT Food Sci Technol 74:219–223

    CAS  Google Scholar 

  • Kumar A, Mendiratta SK, Sen AR, Kandeepan G, Talukder S, Sharma H, Soni A, Irshad A, Kumar S (2015) Preparation and storage stability of meat spread developed from spent hens. Vety World 8(5):651–655

    CAS  Google Scholar 

  • Kumar S, Mendiratta SK, Agarwal RK, Sharma H, Kumar RR (2017) Quality evaluation of mutton nuggets incorporated with optimized level of flaxseed flour. Nutri Food Sci 47(1):67–77

    Google Scholar 

  • Kumar S, Mendiratta SK, Agrawal RK, Sharma H, Singh BP (2018) Anti-oxidant and anti-microbial properties of mutton nuggets incorporated with blends of essential oils. J Food Sci Technol 55(2):821–832

    CAS  PubMed  PubMed Central  Google Scholar 

  • Li H, Ge Y, Luo Z, Zhou Y, Zhang X, Zhang J, Fu Q (2017) Evaluation of the chemical composition, antioxidant and anti-inflammatory activities of distillate and residue fractions of sweet basil essential oil. J Food Sci Technol. https://doi.org/10.1007/s13197-017-2620-x

    Article  PubMed  PubMed Central  Google Scholar 

  • Mele MA, Islam MZ, Baek JP, Kang HM (2017) Quality, storability, and essential oil content of Ligularia fischeri during modified atmosphere packaging storage. J Food Sci Technol. 54(3):743–750

    CAS  PubMed  PubMed Central  Google Scholar 

  • Moradi M, Tajik H, Rohani SMR, Mahmoudian A (2016) Antioxidant and antimicrobial effects of zein edible film impregnated with Zataria multiflora Boiss essential oil and monolaurin. LWT Food Sci Technol 72:37–43

    CAS  Google Scholar 

  • Naveena BM, Sen A, Vaithiyanathan S, Babji Y, Kondaiah N (2008) Comparative efficacy of pomegranate juice, pomegranate rind powder extract and BHT as antioxidants in cooked chicken patties. Meat Sci 80:1304–1308

    CAS  PubMed  Google Scholar 

  • Noor S, Bhat ZF, Kumar S, Mudiyanselage RJ (2018) Preservative effect of Asparagus racemosus: a novel additive for bioactive edible films for improved lipid oxidative stability and storage quality of meat products. Meat Sci 139:207–212

    CAS  PubMed  Google Scholar 

  • Olmedo R, Nepote V, Grosso NR (2014) Antioxidant activity of fractions from oregano essential oils obtained by molecular distillation. Food Chem 156:212–219

    CAS  PubMed  Google Scholar 

  • Raeisi M, Tabaraei A, Hashemi M, Behnampour N (2016) Effect of sodium alginate coating incorporated with nisin, Cinnamomum zeylanicum, and rosemary essential oils on microbial quality of chicken meat and fate of Listeria monocytogenes during refrigeration. Int J Food Microbol 238:139–145

    CAS  Google Scholar 

  • Rodrigues JBS, de Carvalho RJ, de Souza NT, Oliveira KS, Franco OL, Schaffner D, de Souza EL, Magnan M (2017) Effects of oregano essential oil and carvacrol on biofilms of Staphylococcus aureus from food-contact surfaces. Food Cont 3:1237–1246

    Google Scholar 

  • Roy A, Guha P (2015) Development of a novel cup cake with unique properties of essential oil of betel leaf (Piper betle L) for sustainable entrepreneurship. J Food Sci Technol 52(8):4885–4894

    CAS  PubMed  Google Scholar 

  • Sampaio G, Saldanha T, Soares R, Torres E (2012) Effect of natural antioxidant combinations on lipid oxidation in cooked chicken meat during refrigerated storage. Food Chem 135:1383–1390

    CAS  PubMed  Google Scholar 

  • Shadman S, Hosseini SE, Langroudi HE, Shabani S (2017) Evaluation of the effect of a sunflower oil-based nanoemulsion with Zataria multiflora Boiss. essential oil on the physicochemical properties of rainbow trout (Oncorhynchus mykiss) fillets during cold storage. LWT Food Sci Technol 79:511–517

    CAS  Google Scholar 

  • Sharma H, Sharma BD, Talukder S, Ramasamy G (2013) Utilization of tamarind seed powder as bind enhancing agent on the quality of extended restructured mutton chops. Fleischwirtschaft Int 5:58–64

    Google Scholar 

  • Sharma H, Sharma BD, Mendiratta S, Talukder S, Ramasamy G (2014a) Efficacy of flaxseed flour as bind enhancing agent on the quality of extended restructured mutton chops. Asian Aust J Anim Sci 27:247–255

    CAS  Google Scholar 

  • Sharma H, Sharma BD, Talukder S, Mendiratta S, Kumar RR (2014b) Effect of gum acacia on the product characteristics of extended restructured mutton chops. Indian J Anim Res. 48(5):504–508

    Google Scholar 

  • Sharma H, Sharma BD, Talukder S, Ramasamy G (2015a) Utilization of gum tragacanth as bind enhancing agent in extended restructured mutton chops. J Food Sci Technol 52(3):1626–1633

    CAS  PubMed  Google Scholar 

  • Sharma H, Talukder S, Sharma BD, Goswami M, Giriprasad R (2015b) Development and quality assessment of value added chicken fingers. Ind J Poult Sci 50(1):66–71

    Google Scholar 

  • Sharma H, Mendiratta SK, Agrawal RK, Gurunathan K, Kumar S, Singh TP (2017a) Use of various essential oils as bio preservatives and their effect on quality of vacuum packaged fresh chicken sausages under frozen conditions. LWT Food Sci Technol 81:118–127

    CAS  Google Scholar 

  • Sharma H, Mendiratta SK, Agarwal RK, Kumar S, Soni A (2017b) Evaluation of anti-oxidant and anti-microbial activity of various essential oils in fresh chicken sausages. J Food Sci Technol 54(2):279–292

    CAS  PubMed  PubMed Central  Google Scholar 

  • Sharma H, Mendiratta SK, Agrawal RK, Talukder S, Kumar S (2018) Studies on the potential application of various blends of essential oils as antioxidant and antimicrobial preservatives in emulsion based chicken sausages. Br Food J 120(7):1398–1411

    Google Scholar 

  • Soni A, Gurunathan K, Mendiratta SK, Talukder S, Jaiswal RK, Sharma H (2018) Effect of essential oils incorporated edible film on quality and storage stability of chicken patties at refrigeration temperature (4 ± 1°C). J Food Sci Technol 55(9):3538–3546

    CAS  PubMed  PubMed Central  Google Scholar 

  • Sousa SC, Fragoso SP, Penna CRA, Arcanjo NMO, Silva FAP, Ferreira VCS, Baretto DS, Araujo IBS (2017) Quality parameters of frankfurter-type sausages with partial replacement of fat by hydrolyzed collagen. LWT Food Sci Technol 76(B):320–325

    CAS  Google Scholar 

  • Sukcharoen O, Sirirote P, Thanaboripat D (2017) Control of aflatoxigenic strains by Cinnamomum porrectum essential oil. J Food Sci Technol. https://doi.org/10.1007/s13197-017-2731-4

    Article  PubMed  PubMed Central  Google Scholar 

  • Talukder S, Sharma BD, Mendiratta SK, Malav OP, Sharma H, Gokulakrishnan P (2013) Development and evaluation of extended restructured chicken meat block incorporated with colocasia (Colocasia esculenta) flour. J Food Process Technol 4(2):1–9

    Google Scholar 

  • Tarladgis BG, Watts BM, Younathan MT, Dugan L Jr (1960) A distillation method for the quantitative determination of malonaldehyde in rancid foods. J Am Oil Chem Soc 37:44–48

    CAS  Google Scholar 

  • Tepe B, Sokmen M, Akpulat HA, Sokmen A (2005) In vitro antioxidant activities of the methanol extracts of five Allium species from Turkey. Food Chem 92:89–92

    CAS  Google Scholar 

  • Troutt ES, Hunt MC, Johnson DE, Claus JR, Kastner CL, Kropf DH (1992) Characteristics of low-fat ground beef containing texture-modifying ingredients. J Food Sci 57:19–24

    CAS  Google Scholar 

  • Ugalde ML, de Cezaro AM, Vedovatto F, Paroul N, Steffens J, Valduga E, Backes GT, Franceschi E, Cansian RL (2017) Active starch biopolymeric packaging film for sausages embedded with essential oil of Syzygium aromaticum. J Food Sci Technol. https://doi.org/10.1007/s13197-017-2624-6

    Article  PubMed  PubMed Central  Google Scholar 

  • Youn S, Park S, Kim Y, Ahn D (1999) Effect on storage property and quality in meat sausage by added chitosan. J Chitin Chitosan 4:189–195

    Google Scholar 

  • Zengin H, Baysal AH (2014) Antioxidant and antimicrobial activities of thyme and clove essential oils and application in minced beef. J Food Proces Preserv 39(6):1261–1271

    Google Scholar 

  • Zhang S, Zhang M, Fang Z, Liu Y (2017) Preparation and characterization of blended cloves/cinnamon essential oil nanoemulsions. LWT Food Sci Technol 75:316–322

    CAS  Google Scholar 

  • Riveros CG, Nepote V, Grosso NR (2016) Thyme and basil essential oils included in edible coatings as a natural preserving method of oilseed kernels. J Sci Food Agric 96(1):183–191

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We sincerely thank our Director, IVRI for fellowship and grant provided in the institute funded project.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Heena Sharma.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sharma, H., Mendiratta, S.K., Agarwal, R.K. et al. Bio-preservative effect of blends of essential oils: natural anti-oxidant and anti-microbial agents for the shelf life enhancement of emulsion based chicken sausages. J Food Sci Technol 57, 3040–3050 (2020). https://doi.org/10.1007/s13197-020-04337-1

Download citation

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13197-020-04337-1

Keywords

Navigation