Abstract
Idli is one of the most popular naturally fermented breakfast food. In this study, essential oils have been screened for their minimal inhibitory activity against selected lactic acid bacteria (LAB) and yeast strain associated with idli batter fermentation and to identify the best potential bio preservative to preserve the idli batter. Mustard essential oil was found to be the best bio-preservative which showed a biocidal effect at 80 ppm against LAB strains and at 40 ppm against Candida versatilis. The efficacy of mustard essential oil incorporated in the idli batter at 0.1% (w/w) was evaluated by measuring the titratable acidity, pH, viscosity, batter volume, microbial count of idli batter as well as sensory parameters of idli prepared from preserved batter stored at 4 and 30 °C. Unfavorable changes in acidity, batter volume and whey separation of idli batter containing mustard oil were significantly reduced, which resulted in a reduction of sour taste and improved texture of idli. The growth of yeast and LAB was retarded evidenced by decreased microbial counts than control batter, which delayed the deterioration of the batter under both storage conditions. The addition of 0.1% mustard essential oil in the optimally fermented idli batter extended its shelf life to 5 days when stored at 30 °C and 30 days at 4 °C.
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References
Burt S (2004) Essential oils: their antibacterial properties and potential applications in foods—a review. Int J Food Microbiol 94:223–253
Chelliah R, Ramakrishnan SR, Premkumar D, Antony U (2016) Bio-fortification and shelf-life extension of idli batter using curry leaves (Murraya koenigii). J Food Sci Technol 53:2851–2862. https://doi.org/10.1007/s13197-016-2264-2
Dhamija A (2017) iD Fresh Food has built a brand out of ready-made idli-dosa batter. http://www.forbesindia.com/article/work-in-progress/id-fresh-food-has-built-a-brand-out-of-readymade-idlidosa-batter/46717/1. Accessed 15 Jan 2018
Durgadevi M, Shetty PH (2012) Effect of ingredients on sensory profile of idli. J Food Sci Technol 51:1773–1783. https://doi.org/10.1007/s13197-012-0686-z
El Fayoumy RA, Pendleton P, El-Fallal AA et al (2017) Allyl isothiocyanate release from edible laminaria japonica for time-dependent growth deactivation of foodborne pathogens: I: Micrococcus luteus, Bacillus subtilis, and Listeria monocytogenes. Food Bioprocess Technol 10:1562–1573. https://doi.org/10.1007/s11947-017-1925-0
Holley RA, Patel D (2005) Improvement in shelf-life and safety of perishable foods by plant essential oils and smoke antimicrobials. Food Microbiol 22:273–292
Inatsu Y, Bari ML, Kawasaki S, Kawamoto S (2005) Effectiveness of some natural antimicrobial compounds in controlling pathogen or spoilage bacteria in lightly fermented Chinese cabbage. J Food Sci. https://doi.org/10.1111/j.1365-2621.2005.tb08323.x
Iyer BK, Ananthanarayan L (2008) Effect of α-amylase addition on fermentation of idli—a popular south Indian cereal—Legume-based snack food. LWT Food Sci Technol 41:1053–1059
Kalemba D, Kunicka A (2003) Antibacterial and antifungal properties of essential oils. Curr Med Chem 10:813–829. https://doi.org/10.2174/0929867033457719
Ko JA, Kim WY, Park HJ (2012) Effects of microencapsulated Allyl isothiocyanate (AITC) on the extension of the shelf-life of Kimchi. Int J Food Microbiol 153:92–98
Lakshmi OB (2010) Antibacterial activity of black pepper (Piper nigrum Linn.) with special reference to its mode of action on bacteria. Indian J Nat Prod Resour 1(2):213–215
Lin CM, Preston JF III, Wei CI (2000) Antibacterial mechanism of allyl isothiocyanate. J Food Prot 63:727–734
Nielsen PV, Rios R (2000) Inhibition of fungal growth on bread by volatile components from spices and herbs, and the possible application in active packaging, with special emphasis on mustard essential oil. Int J Food Microbiol 60:219–229
Nisha P, Ananthanarayan L, Singhal RS (2005) Effect of stabilizers on stabilization of idli (traditional south Indian food) batter during storage. Food Hydrocoll 19:179–186
Olaimat AN, Holley RA (2016) Inhibition of Listeria monocytogenes on cooked cured chicken breasts by acidified coating containing allyl isothiocyanate or deodorized oriental mustard extract. Food Microbiol 57:90–95
Reddy NR, Sathe K, Pierson MD, Salunkhe DK (1982) Idli, an indian fermented food: a review. J Food Qual 5:89–101
Ribeiro-santos R, Andrade M, Sanches-silva A (2017) Essential oils for food application: natural substances with established biological activities. Food Bioprocess Technol 10:1562–1573. https://doi.org/10.1007/s11947-017-1948-6
Saravanan C, Shetty PKH (2016) Isolation and characterization of exopolysaccharide from Leuconostoc lactis KC117496 isolated from idli batter. Int J Biol Macromol 90:100–106
Saravanan C, Gopu V, Shetty PH (2015) Diversity and functional characterization of microflora isolated from traditional fermented food idli. J Food Sci Technol 52:7425–7432
Shelef A (1983) Antimicrobial effects of spices. J Food Saf 6:29–44
Shofran BG, Purrington ST, Breidt F, Fleming HP (1998) Antimicrobial properties of sinigrin and its hydrolysis products. J Food Sci 63:621–624. https://doi.org/10.1111/j.1365-2621.1998.tb15798.x
Shrivastava N, Ananthanarayan L (2015) Use of the backslopping method for accelerated and nutritionally enriched idli fermentation. J Sci Food Agric 95:2081–2087
Sridevi J, Halami PM, Vijayendra SVN (2010) Selection of starter cultures for idli batter fermentation and their effect on quality of idlis. J Food Sci Technol 47:557–563
Steinkraus K (1995) Handbook of indigenous fermented foods, revised and expanded. CRC Press, Boca Raton
Wiegand I, Hilpert K, Hancock REW (2008) Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc 3:163–175
William H (1980) Official methods of analysis of the Association of Official Analytical Chemists. http://agris.fao.org/agris-search/search.do?recordID=US19830838740. Accessed 21 Dec 2017
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One of the authors Mr. Baburaj Regubalan is grateful to University Grants commission—Basic Scientific Research (UGGC-BSR) for the scholarship supported from the Government of India.
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Regubalan, B., Ananthanarayan, L. Shelf life improvement of idli batter by addition of mustard essential oil as bio-preservative. J Food Sci Technol 55, 3417–3426 (2018). https://doi.org/10.1007/s13197-018-3247-2
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DOI: https://doi.org/10.1007/s13197-018-3247-2