Abstract
Basella is an important green leafy vegetable species of Chenopodiaceae family and is known for its medicinal properties. Hydroxy-benzoic acids, hydroxy-cinnamic acids and flavones groups were identified and characterized from the aqueous stem extracts of B. alba and B. rubra species. Higher values of phenolics as well as antioxidant activity were noted from B. alba species extracts. The evaluation of the cytoxicity of these extracts on A431 (epidermoid carcinoma), Hep G2 (hepatocellular carcinoma) and MG 63 (osteosarcoma) cells indicated anti-proliferative activity against all the cell lines. B. alba extract showed higher anti-proliferative activity (37.95–84.86%). Chick embryo chorioallantoic membrane (CAM) assay revealed inhibition of neo-vessels formation. Significant suppression was found with extracts of B. alba at 7 mg/ml compared to that of B. rubra. This is the first study to report the anti-angiogenic activity of Basella species. These studies indicate that Basella sps can be used as a source of natural antioxidants and can be of high significance in pharmaceutical and nutraceutical industries.
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Anatharaju PG, Gowda PC, Vimalambike MG, Madhunapantula SV (2016) An overview on the role of dietary phenolics for the treatment of cancers. Nutr J 15:1–16
Anish K, Adinpunya M (2016) Methoxybenzaldehydes in plants: insight to the natural resources, isolation, application and biosynthesis. Curr Sci 111:1325–1334
Balasundram N, Sundran K, Samman S (2006) Phenolic Compounds in plants and agri-industrial by-products: antioxidant activity, occurrence and potential uses. Food Chem 99:191–203
Bertin RL, Gonzaga LV, Graciele da Silva CB, Azevedo MS, Maltez HF, Heller M, Micke GA (2014) Nutrient composition and identification/quantification of major phenolic compounds in Sarcocornia ambigua (Amaranthaceae) using HPLC-ESI-MS/MS. Food Res Int 55:404–411
Biswas D, Roymon MG (2013) LC/TOF/ESI/MS detection of bioactive compounds present in leaf and bark extract of Acacia Arabica. Recent Res Sci Technol 5:37–40
Brand- Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 28:25–30
Brooks PC, Montgomery AM, Cheresh DA (1999) Use of the 10-day-old chick embryo model for studying angiogenesis. Methods Mol Bio 129:257–269
Chun-Hua C, Ting-Tsz O, Mon-Yuan Y, Chi-Chou H, Chau-Jong W (2016) Nelumbo nucifera Gaertn leaves extracts inhibits the angiogenesis and metastasis of breast cancer cells by down regulation connective tissue growth factor (CTGF) mediated P13K/AKT/ERK signaling. J Ethnopharmacol 88:111–122
Erşan S, Ustundag OG, Carle R, Schweiggert RM (2016) Identification of phenolic compounds in red and green pistachio (Pistacia vera L.) Hulls (Exo- and Mesocarp) by HPLC-DAD-ESI-(HR)-MSn. J Agric Food Chem 64:5334–5344
Gupta S, Prakash J (2009) Studies on Indian green leafy vegetables for their antioxidant activity. Plant Foods Hum Nutr 64:39–45
Habib S, Vahid N, Mehdi T, Hasan E (2017) Ultrasound- assisted extraction process of phenolic antioxidants from Olive leaves: a nutraceutical study using RSM and LC-ESI-DAD-MS. J Food Sci Technol 54:2361–2371
Karam MC, Petit J, Zimmer D, Djantou EB, Scher J (2016) Effects of drying and grinding in production of fruit and vegetable powders: a review. J Food Eng 188:32–49
Khanam UKS, Oba S, Yanase E, Murakami Y (2012) Phenolic acids, flavonoids and total antioxidant capacity of selected leafy vegetables. J Funct Foods 4:979–987
Kilari BP, Kotakadi VS, Penchalaneni J (2016) Anti-proliferative and apoptotic effects of Basella rubra (L.) Against 1, 2-Dimethyl Hydrazine-induced colon carcinogenesis in rats. Asian Pac J Cancer Prev 17:73–80
Kumar BR (2017) Application of HPLC and ESI-MS techniques in the analysis of phenolic acids and flavonoids from green leafy vegetables (GLVs). J Pharm Anal 7:349–364
Kumar S, Prasad AK, Iyer SV, Vaidya SK (2013) Systematic pharmacognostical, phytochemical and pharmacological review on an ethno medicinal plant, Basella alba L. J Pharmacogn Phytother 5:53–58
Kumar SS, Manoj P, Giridhar P, Shrivastava R, Bharadawaj M (2015a) Fruit extracts of Basella rubra that are rich in bioactives and betalains exhibit antioxidant activity and cytotoxicity against human cervical carcinoma cells. J Funct Foods 15:509–515
Kumar SS, Manoj P, Shetty NP, Prakash M, Giridhar P (2015b) Characterization of major betalain pigments -gomphrenin, betanin and isobetanin from Basella rubra L. fruit and evaluation of efficacy as a natural colourant in product (ice cream) development. J Food Sci Technol 52:4994–5002
Kumar SS, Manoj P, Giridhar P (2015c) A method for red-violet pigments extraction from fruits of Malabar spinach (Basella rubra) with enhanced antioxidant potential under fermentation. J Food Sci Technol 52:3037–3043
Kumar SS, Manoj P, Nimisha G, Giridhar P (2016) Phytoconstituents and stability of betalains in fruit extracts of Malabar spinach (Basella rubra L.). J Food Sci Technol 53:4014–4022
Olajire AA, Azeez L (2011) Total antioxidant activity, phenolic, flavonoids and ascorbic acid contents of Nigerian vegetables. Afr J Food Sci 2:22–29
Ostrowski W, Wojakowaska A, Grajzer M, Stobiecki M (2014) Mass spectrometric behavior of phenolic acids standards and their analysis in the plant samples with LC/ESI/MS system. J Chromatogr B 967:21–27
Oyaizu M (1986) Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 44:307–315
Padmavati M, Reddy AR (1999) Flavonoid pathway and cereal defense response: an emerging trend in crop biotechnology. J Plant Biochem Biotechnol 8:15–20
Padmavati M, Sakthivel N, Thara KV, Reddy AR (1998) Differential sensitivity of rice pathogens to growth inhibition by flavonoids. Phytochemistry 46:499–502
Piazzon A, Vrhovsek U, Masuero D, Mattivi F, Mandoj F, Nardini M (2012) Antioxidant activity of phenolic acids and their metabolites: synthesis and antioxidant properties of the sulfate derivatives of ferulic and caffeic acids and of the acyl glucuronide of ferulic acid. J Agric Food Chem 60:12312–12323
Priya K, Gupta A, Mahajan S, Agnihotri RK, Sharma R (2015) Evaluation of antimicrobial properties of Basella rubra methanolic extracts on selected microorganisms. Int J Pharma Sci Drug Res 6:334–336
Razzaghi-Asl N, Garride J, Khazraei H, Borges G, Firuzi D (2013) Antioxidant properties of hydroxycinnamic acids: a review of structure activity relationships. Curr Med Chem 20:4436–4450
Santi MM, Dipjyoti C (2013) Mass spectrometric detection of Phenolic acids. In: Ramawat KG, Merillion JM (eds) Natural products: Phytochemistry, Botany and Metabolism of Alkaloids, Phenolics and Terpenes. Springer, Berlin, Heidelberg, New York, pp 2047–2057
Shenbhagaraman R, Jagadish LK, Premalatha K, Kaviyarasan V (2012) Optimization of extracellular glucan production from Pleuotus eryngii and its impact on angiogenesis. Int J Biol Macromolec 50:957–964
Siddhuraju P, Becker K (2003) Antioxidant properties of various solvent extracts of total phenolic constituents from three different agro climatic origins of drumstick tree (Moringa oleifera Lam.) leaves. J Agric Food Chem 51:2144–2155
Slavin JL, Lloyd B (2012) Health benefits of fruits and vegetables. Adv Nutr 3:506–516
Stintzing FC, Kammerer D, Schieber A, Adama H, Nacoulma OG, Carle R (2004) Betacyanins and Phenolic compounds from Amaranthus spinosus L. And Boerhavia erecta L. Zeitschrift fur Naturforschung 59:1–8
Tai-Ping F, Ju-Ching Y, Kar-Wah L, Yue PYK, Wong RNS (2006) Angiogenesis: from plants to blood vessels. Trends Pharmacol Sci 27:297–309
Zhishen J, Mencheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effect on superoxide radicals. Food Chem 64:555–559
Acknowledgements
The authors acknowledge the funding support provided by Ministry of Human Resource Development (MHRD) New Delhi, Government of India. We gratefully acknowledge the support from Department of Chemistry, IIT Kharagpur and School of Medicine Science and Technology, IIT Kharagpur.
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Kumar, B.R., Anupam, A., Manchikanti, P. et al. Identification and characterization of bioactive phenolic constituents, anti-proliferative, and anti-angiogenic activity of stem extracts of Basella alba and rubra. J Food Sci Technol 55, 1675–1684 (2018). https://doi.org/10.1007/s13197-018-3079-0
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DOI: https://doi.org/10.1007/s13197-018-3079-0