Abstract
The present study was performed to evaluate the phytochemicals profiles of some cereal milling by-products such as wheat (bran, germ and shorts), rice (bran, germ and husk) and corn (bran, germ and germ meal) to assess their potentiality as bioactive compounds sources. Distilled water, ethanol, methanol, and acetone separately were used as solvents for the extraction of phytochemicals compounds. The antioxidant activity (AOA), total phenolics content (TPC), and total flavonoids content (TFC) of the extracts were investigated using various in vitro assays. The results showed that tannins content was ranged from 113.4 to 389.5 (mg/100 g sample).The study revealed that TPC and TFC of cereal by-products extracts were significantly different for various solvents. TPC content varied from 366.1 to 1924.9 mg/100 g and TFC content varied from 139.3 to 681.6 mg/100 g. High carotenoids content was observed for corn germ meal and minimum for wheat bran. Distilled water, ethanol and methanol extracts showed significantly different antioxidant activity. Significant variations were observed with regard to AOA of different cereal by-products by using various solvents. The ethanol and methanol were observed to be the best solvents to extract phenolic compounds and antioxidant activity, while acetone extract showed less efficiency. Also, the cereal milling by-products were rich in bioactive compounds and could be used as a value added products.
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Abbreviations
- AOA:
-
Antioxidant activity
- TPC:
-
Total phenolic content
- TFC:
-
Total flavonoids content
References
AACC (2000) Approved methods of the AACC-method, 10th ed, USA
Abdel-Aal ESM, Hucl P, Sosulski FW et al (2001) Screening spring wheat for midge resistance in relation to ferulic acid content. J Agric Food Chem 49:3559–3566. https://doi.org/10.1021/jf010027h
Abozed SS, El-kalyoubi M, Abdelrashid A, Salama MF (2014) Total phenolic contents and antioxidant activities of various solvent extracts from whole wheat and bran. Ann Agric Sci 59:63–67. https://doi.org/10.1016/j.aoas.2014.06.009
Adom KK, Sorrells ME, Rui HL (2005) Phytochemicals and antioxidant activity of milled fractions of different wheat varieties. J Agric Food Chem 53:2297–2306. https://doi.org/10.1021/jf048456d
Al-Okbi SY, Hussein AMS, Hamed IM et al (2014) Chemical, rheological, sensorial and functional properties of gelatinized corn–rice bran flour composite corn flakes and tortilla chips. J Food Process Preserv 38:83–89. https://doi.org/10.1111/j.1745-4549.2012.00747.x
Benzie IFF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239:70–76. https://doi.org/10.1006/abio.1996.0292
Broekaert WF, Courtin CM, Verbeke K et al (2011) Prebiotic and other health-related effects of cereal-derived arabinoxylans, arabinoxylan-oligosaccharides, and xylooligosaccharides. Crit Rev Food Sci Nutr 51:178–194. https://doi.org/10.1080/10408390903044768
Ciccoritti R, Taddei F, Nicoletti I et al (2017) Use of bran fractions and debranned kernels for the development of pasta with high nutritional and healthy potential. Food Chem 225:77–86. https://doi.org/10.1016/j.foodchem.2017.01.005
Crittenden R, Karppinen S, Ojanen S et al (2002) In vitro fermentation of cereal dietary fibre carbohydrates by probiotic and intestinal bacteria. J Sci Food Agric 82:781–789. https://doi.org/10.1002/jsfa.1095
Dent M, Dragovi V, Peni M, Brn M (2013) The effect of extraction solvents, temperature and time on the composition and mass fraction of polyphenols in dalmatian wild sage (Salvia officinalis L.) extracts. Food Technol Biotechnol 51:84–91. https://doi.org/10.1186/2251-6581-13-11
Djilas S, Canadanovic-Brunet J, Cetkovic G (2009) By-products of fruits processing as a source of phytochemicals. Chem Ind Chem Eng Q 15:191–202. https://doi.org/10.2298/CICEQ0904191D
Do QD, Angkawijaya AE, Tran-Nguyen PL et al (2014) Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. J Food Drug Anal 22:296–302. https://doi.org/10.1016/j.jfda.2013.11.001
Dykes L, Rooney LW (2006) Sorghum and millet phenols and antioxidants. J Cereal Sci 44:236–251. https://doi.org/10.1016/j.jcs.2006.06.007
Heinio RL, Liukkonen KH, Myllymäki O et al (2008) Quantities of phenolic compounds and their impacts on the perceived flavour attributes of rye grain. J Cereal Sci 47:566–575. https://doi.org/10.1016/j.jcs.2007.06.0
Hentschel V, Kranl K, Hollmann J et al (2002) Spectrophotometric determination of yellow pigment content and evaluation of carotenoids by high-performance liquid chromatography in durum wheat grain. J Agric Food Chem 50:6663–6668. https://doi.org/10.1021/jf025701p
Iloki-Assanga SB, Lewis-Luján LM, Lara-Espinoza CL et al (2015) Solvent effects on phytochemical constituent profiles and antioxidant activities, using four different extraction formulations for analysis of Bucida buceras L. and Phoradendron californicum. BMC Res Notes 8:396. https://doi.org/10.1186/s13104-015-1388-1
Kim KH, Tsao R, Yang R, Cui SW (2006) Phenolic acid profiles and antioxidant activities of wheat bran extracts and the effect of hydrolysis conditions. Food Chem 95:466–473. https://doi.org/10.1016/j.foodchem.2005.01.032
Kosar M, Dorman HJD, Hiltunen R (2005) Effect of an acid treatment on the phytochemical and antioxidant characteristics of extracts from selected Lamiaceae species. Food Chem 91:525–533. https://doi.org/10.1016/j.foodchem.2004.06.029
Li Y, Ma D, Sun D et al (2015) Total phenolic, flavonoid content, and antioxidant activity of flour, noodles, and steamed bread made from different colored wheat grains by three milling methods. Crop J 3:328–334. https://doi.org/10.1016/j.cj.2015.04.004
Liu M, Li XQ, Weber C, Lee CY, Brown J, Liu RH (2002) Antioxidant and antiproliferative activities of raspberries. J Agric Food Chem 50:2926–2930. https://doi.org/10.1021/jf0111209
Liu F, Chen Z, Shao J et al (2017) Effect of fermentation on the peptide content, phenolics and antioxidant activity of defatted wheat germ. Food Biosci 20:141–148. https://doi.org/10.1016/j.fbio.2017.10.002
Liyana-Pathirana CM, Shahidi F (2007) Antioxidant and free radical scavenging activities of whole wheat and milling fractions. Food Chem 101:1151–1157. https://doi.org/10.1016/j.foodchem.2006.03.016
Masisi K, Beta T, Moghadasian MH (2016) Antioxidant properties of diverse cereal grains: a review on in vitro and in vivo studies. Food Chem 196:90–97. https://doi.org/10.1016/j.foodchem.2015.09.021
Nam SH, Choi SP, Kang MY et al (2005) Antioxidative, antimutagenic, and anticarcinogenic activities of rice bran extracts in chemical and cell assays. J Agric Food Chem 53:816–822. https://doi.org/10.1021/jf0490293
Ndolo VU, Beta T (2013) Distribution of carotenoids in endosperm, germ, and aleurone fractions of cereal grain kernels. Food Chem 139:663–671. https://doi.org/10.1016/j.foodchem.2013.01.014
Osman H, Rahim AA, Isa NM, Bakhir NM (2009) Antioxidant activity and phenolic content of Paederia foetida and Syzygium aqueum. Molecules 14:970–978. https://doi.org/10.3390/molecules14030970
Panfili G, Fratianni A, Irano M (2004) Improved normal-phase high-performance liquid chromatography procedure for the determination of carotenoids in cereals. J Agric Food Chem 52:6373–6377. https://doi.org/10.1021/jf0402025
Price ML, Van Scoyoc S, Butler LG (1978) A critical evaluation of the vanillin reaction as an assay for tannin in sorghum grain. J Agric Food Chem 26:1214–1218. https://doi.org/10.1021/jf60219a031
Saharan P, Sadh PK, Singh Duhan J (2017) Comparative assessment of effect of fermentation on phenolics, flavanoids and free radical scavenging activity of commonly used cereals. Biocatal Agric Biotechnol 12:236–240. https://doi.org/10.1016/j.bcab.2017.10.013
Serafini M, Bellocco R, Wolk A, Ekström AM (2002) Total antioxidant potential of fruit and vegetables and risk of gastric cancer. Gastroenterology 123:985–991. https://doi.org/10.1053/gast.2002.35957
Stefanello FS, dos Santos CO, Bochi VC et al (2018) Analysis of polyphenols in brewer’s spent grain and its comparison with corn silage and cereal brans commonly used for animal nutrition. Food Chem 239:385–401. https://doi.org/10.1016/j.foodchem.2017.06.130
Sun T, Xu Z, Godber JS, Prinyawiwatkul W (2006) Capabilities of oat extracts in inhibiting cholesterol and long chain fatty acid oxidation during heating. Cereal Chem 83:451–454. https://doi.org/10.1094/CC-83-0451
Thakur S, Singh N, Kaur A, Singh B (2017) Effect of extrusion on physicochemical properties, digestibility, and phenolic profiles of grit fractions obtained from dry milling of normal and waxy corn. J Food Sci 82:1101–1109. https://doi.org/10.1111/1750-3841.13692
Wang SY, Chen CT (2010) Effect of allyl isothiocyanate on antioxidant enzyme activities, flavonoids and post-harvest fruit quality of blueberries (Vaccinium corymbosum L., cv. Duke). Food Chem 122:1153–1158. https://doi.org/10.1016/j.foodchem.2010.03.106
Yu L, Haley S, Perret J et al (2002) Free radical scavenging properties of wheat extracts. J Agric Food Chem 50:1619–1624. https://doi.org/10.1021/jf010964p
Zhang G, He L, Hu M (2011) Optimized ultrasonic-assisted extraction of flavonoids from Prunella vulgaris L. and evaluation of antioxidant activities in vitro. Innov Food Sci Emerg Technol 12:18–25. https://doi.org/10.1016/j.ifset.2010.12.003
Zhao HM, Guo XN, Zhu KX (2017) Impact of solid state fermentation on nutritional, physical and flavor properties of wheat bran. Food Chem 217:28–36. https://doi.org/10.1016/j.foodchem.2016.08.062
Zhou K, Yu L (2004a) Antioxidant properties of bran extracts from trego wheat grown at different locations. J Agric Food Chem 52:1112–1117. https://doi.org/10.1021/jf030621
Zhou K, Yu L (2004b) Effects of extraction solvent on wheat bran antioxidant activity estimation. LWT Food Sci Technol 37:717–721. https://doi.org/10.1016/j.lwt.2004.02.008
Zhou K, Su L, Yu LL (2004) Phytochemicals and antioxidant properties in wheat bran. J Agric Food Chem 52:6108–6114. https://doi.org/10.1021/jf049214g
Złotek U, Mikulska S, Nagajek M, Świeca M (2016) The effect of different solvents and number of extraction steps on the polyphenol content and antioxidant capacity of basil leaves (Ocimum basilicum L.) extracts. Saudi J Biol Sci 23:628–633. https://doi.org/10.1016/j.sjbs.2015.08.002
Acknowledgements
This work was supported by Danish Agency for Higher Education and Egyptian Cultural Affairs and Missions (Ministry of Higher Education). Sayed thanks to the Design and Consumer Behaviour section, Faculty of Science, University of Copenhagen, Denmark for the Guest Ph.D. student position.
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Smuda, S.S., Mohsen, S.M., Olsen, K. et al. Bioactive compounds and antioxidant activities of some cereal milling by-products. J Food Sci Technol 55, 1134–1142 (2018). https://doi.org/10.1007/s13197-017-3029-2
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DOI: https://doi.org/10.1007/s13197-017-3029-2