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Encapsulation of eugenyl acetate in PHBV using SEDS technique and in vitro release evaluation

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Abstract

Eugenyl acetate obtained via enzymatic esterification using Lipozyme TL IM enzyme was encapsulated in biopolymer poly(3-hydroxybutyrate-co-hydroxyvalerate) (PHBV) through solution-enhanced dispersion by supercritical fluids (SEDS). Produced particles were characterized by SEM and confocal microscopy techniques and in addition in vitro release assays were performed in isopropanol and ethyl acetate. Experimental micronization conditions comprised 8 and 10 MPa, 308 and 313 K and eugenyl acetate concentration ranging from 5 to 20 mg mL−1, keeping PHBV concentration constant (20 mg mL−1 in dichloromethane). The maximum encapsulation efficiency was 58.0 % for 5 mg mL−1of eugenyl acetate at 8 MPa and 308 K. The morphology of the encapsulated particles for most of the trials was spherical, with particle size ranging from 0.061 to 0.276 μm. Regarding the release in ethyl acetate and isopropanol solvents the higher the affinity of the encapsulated ester of these solvents, the faster the release was observed. These results demonstrate the importance of essential clove oil esterification reaction and encapsulation of the ester by SEDS method so that this encapsulated ester can be used in different industrial applications.

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Acknowledgments

The authors thank CNPq, CAPES, FAPESC/SC and MCTI-FINEP for financial support and scholarships.

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Authors and Affiliations

  1. Department of Chemical and Food Engineering, UFSC, Florianópolis, SC, Brazil

    Raquel A. Loss, Gabriela N. Pereira, Daiane L. Boschetto, Gean S. P. Aguiar, Juliana R. Machado, Lorenzo M. P. C. Chaves, Maria J. A. Silva, Débora Oliveira & J. Vladimir Oliveira

  2. Universidade Estadual do Estado de Mato Grosso, Barra do Bugres, MT, 78390-000, Brazil

    Raquel A. Loss

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  1. Raquel A. Loss
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  2. Gabriela N. Pereira
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  3. Daiane L. Boschetto
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  4. Gean S. P. Aguiar
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  5. Juliana R. Machado
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  6. Lorenzo M. P. C. Chaves
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  7. Maria J. A. Silva
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  8. Débora Oliveira
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  9. J. Vladimir Oliveira
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Correspondence to J. Vladimir Oliveira.

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Loss, R.A., Pereira, G.N., Boschetto, D.L. et al. Encapsulation of eugenyl acetate in PHBV using SEDS technique and in vitro release evaluation. J Food Sci Technol 53, 3859–3864 (2016). https://doi.org/10.1007/s13197-016-2362-1

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  • DOI: https://doi.org/10.1007/s13197-016-2362-1

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