Abstract
Nagpur mandarin orange (Citrus reticulata) peels were subjected to treatment with nanobiocatalysts in the form of cellulase and pectinase immobilized magnetic nanoparticles (MNPs). MNPs (Fe3O4) with average diameter in range of 40–90 nm were immobilized with cellulase and pectinase through APTES and glutaraldehyde. Treatment followed by extraction into organic solvents resulted in 8-9 fold increase in extraction of carotenoidic pigments compared to use of free enzymes. Optimum pH and temperature for the process were determined to be 5.0 and 50 °C, respectively. The nanobiocatalysts could be reused across three cycles with only 15 % drop in yield per cycle. Dinitrosalicylic acid assays showed that superior peel hydrolysis also led to greatest extent of pigment extraction.
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References
Aravantinos-Zafiris G, Oreopoulou V, Tzia C, Thomopoulos CD (1992) Utilisation of orange by-products orange peel carotenoids. J Sci Food Agric 59(1):77–79
Chamorro S, Viveros A, Alvarez L, Vega E, Brenes A (2012) Changes in polyphenol and polysaccharide content of grape seed extract and grape pomace after enzymatic treatment. Food Chem 133(2):308–314
Cinar I (2005) Effects of cellulase and pectinase concentrations on the colour yield of enzyme extracted plant carotenoids. Process Biochem 40:945–949
Delgado Vargas F, Pareds Lopez O (1997) Effects of enzymatic treatments on carotenoid extraction from marigold flowers (Tagetes erecta). Food Chem 58:255–258
Dominguez H, Nunez MJ, Lema JM (1994) Enzymatic pre-treatment to enhance oil extraction from fruits and oil seeds: a review. Food Chem 49(3):271–286
Fernandez K, Vega M, Aspe E (2015) An enzymatic extraction of proanthocyanidins from Pais grape seeds and skins. Food Chem 168:7–13
Fraser ML, Lee AH, Binns CW (2005) Lycopene and prostate cancer: emerging evidence. Expert Rev Anticancer Ther 5:847–854
Ghose TK (1987) Measurement of cellulase activities. Pure Appl Chem 59(2):257–268
Gnayfeed MH, Daood HG, Illes V, Biacs PA (2001) Supercritical CO2 and subcritical propane extraction of pungent paprika and quantification of carotenoids, tocopherols and capsaicinoids. J Agric Food Chem 49:2761–2766
Goodwin TW (1980) Biochemistry of the carotenoids, vol 1: Plants, 2nd edn. Chapman and Hall, New York
Goodwin TW, Britton G (1988) Distribution and analysis of carotenoids. In: Goodwin TW (ed) Plant pigments. Academic Press, London, pp 62–132
Grohman K, Baldwin EA (1992) Hydrolysis of orange peel with pectinase and cellulase enzymes. Biotechnol Lett 14:1169–1174
Iyer PV, Ananthanarayan L (2008) Enzyme stability and stabilization-aqueous and non-aqueous environment. Process Biochem 43:1019–1032
Jordan J, Challa SSRK, Theegala C (2011) Preparation and characterization of cellulase-bound magnetite nanoparticles. J Mol Catal B Enzym 68:139–146
Khoshnevisan K, Bordbar A-K, Zare D, Davoodi D, Noruzi M, Barkhi M, Tabatabaei M (2011) Immobilization of cellulase enzyme on superparamagnetic nanoparticles and determination of its activity and stability. Chem Eng J 171:669–673
Koh I, Wang X, Varghese B, Isaacs L, Ehrman SH, English DS (2006) Magnetic iron oxide nanoparticles for biorecognition: evaluation of surface coverage and activity. J Phys Chem B 110:1553–1558
Kouassi GK, Irudayaraj J, McCarty G (2005) Examination of cholesterol oxidase attachment to magnetic nanoparticles. J Nanobiotechnol 3:1
Krinsky NI (1994) The biological properties of carotenoids. Pure Appl Chem 66:1003–1010
Kuo CH, Liu YC, Chang CM, Chen JH, Chang C, Shieh CJ (2012) Optimum conditions for lipase immobilization on chitosan-coated Fe3O4 nanoparticles. Carbohydr Polym 87:2538–2545
Lei Z, Bi S, Hu B, Yang H (2007) Combined magnetic and chemical covalent immobilization of pectinase on composite membranes improves stability and activity. Food Chem 105:889–896
Mehta RV, Upadhyay RV, Charles SW, Ramchand CN (1997) Direct binding of protein to magnetic particles. Biotechnol Tech 11(7):493–496
Miller GL (1959) Use of dinitrosalicylic acid reagent for determinations of reducing sugar. Anal Chem 31:426–428
Nagodawithana T, Reed G (1993) Enzymes in processing, 3rd edn. Academic Press, San Diego
Parka HJ, McConnella JT, Boddohib S, Kipperb MJ, Johnson PA (2011) Synthesis and characterization of enzyme-magnetic nanoparticle complexes: effect of size on activity and recovery. Colloids Surf B Biointerfaces 83:198–203
Polizzi KM, Bommarius AS, Broering JM, Chaparro-Riggers JF (2007) Stability of biocatalysts. Curr Opin Chem Biol 11:220–225
Salvatore Lenucci M, De Caroli M, Marrese PP, Iurlaro A, Recio L, Bohm V, Dalessandro G, Piro G (2015) Enzyme-aided extraction of lycopene from high-pigment tomato cultivars by supercritical carbon dioxide. Food Chem 170:193–202
Stahl W, Sies H (2005) Bioactivity and protective effects of natural carotenoids. Biochim Biophys Acta Mol Basis Dis 1740:101–107
Wilkins MR, Widmer WW, Grohmann K, Cameron RG (2007) Hydrolysis of grapefruit peel waste with cellulase and pectinase enzymes. Bioresour Technol 98:1596–1601
Wrolstad RE, Acree TE, Decker EA, Penner MH, Reid DS, Schwartz SJ, Shoemaker CF, Smith D, Sporns P (eds) (2005) Handbook of food analytical chemistry: pigments, colorants, flavor, texture and bioactive food components. Wiley, London
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Kumar, S., Sharma, P., Ratrey, P. et al. Reusable nanobiocatalysts for the efficient extraction of pigments from orange peel. J Food Sci Technol 53, 3013–3019 (2016). https://doi.org/10.1007/s13197-016-2272-2
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DOI: https://doi.org/10.1007/s13197-016-2272-2