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Impact of dehulling and germination on nutrients, antinutrients, and antioxidant properties in horsegram

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Abstract

The changes in chemical composition, antioxidant activity and minerals content of horse gram seed after dehulling and germination of 12 advance lines were investigated. Dehulled samples had a higher protein content compared with the raw and germinated. Total soluble sugars (TSS) content increased significantly (p ≤ 0.05) after dehulling (29.31 %) and germination (98.73 %) whereas, the total lipids increased (10.98 %) significantly (p ≤ 0.05) after dehulling and decreased (36.41 %) significantly (p ≤ 0.05) after germination. Dehulling and germination significantly decreased the amount of phytic acid (PA), tannin (TN) and oxalic acid (OA). Trypsin inhibitor units decreased (26.79 %) significantly (p ≤ 0.05) after germination. The minerals (Ca, Fe and Cu) composition of the germinated horsegram flour samples was significantly higher than the raw and dehulled flour. The functional properties of flours were studied and found that the bulk density (11.85 %) and oil absorption capacity (18.92 %) significantly increased after germination. Raw samples followed by germinated samples showed the highest concentrations of phytochemicals responsible for the antioxidant activity and also the antioxidant capacities. principal component analysis revealed that in case of dehulled samples; TN, polyphenols, DPPH and ABTS radical inhibition, TSS, total antioxidant, OA, protein, FRAP value, Ca and Zn had positive correlation among themselves while in case of germinated samples, protein, oil absorption capacity, FRAP value, OA, total flavonoids, DPPH radical inhibition, Ca and Cu had positive correlation among themselves. Present study suggest that germination combined with dehulling process improved quality of horsegram by enhancing the nutritive value and reducing the antinutrients.

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Acknowledgments

The authors are grateful to Indian Council of Agricultural Research (ICAR), for financial support to carry out this work at Vivekanand Parvatiya Krishi Anusandhan Sansthan (VPKAS), Almora (Uttarakhand) 263601.

Conflict of interest

The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. ICAR-Vivekananda Institute of Hill Agriculture, Almora, Uttarakhand, India

    R. S. Pal, Anuradha Bhartiya, R. ArunKumar, Lakshmi Kant, J. P. Aditya & J. K. Bisht

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  1. R. S. Pal
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  2. Anuradha Bhartiya
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  3. R. ArunKumar
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  4. Lakshmi Kant
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  5. J. P. Aditya
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  6. J. K. Bisht
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Correspondence to R. S. Pal.

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Highlights

➢ Dehulling improves the protein, sugars and lipids content, significantly.

➢ Tannin, oxalic acid and phytic acid reduced significantly in dehulled samples over raw.

➢ Germination improved the nutrients content in horsegram, significantly.

➢ Anti-nutritive compounds were significantly decreased after germination.

➢ Antioxidant capacities decreased significantly after dehulling and germination.

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Pal, R.S., Bhartiya, A., ArunKumar, R. et al. Impact of dehulling and germination on nutrients, antinutrients, and antioxidant properties in horsegram. J Food Sci Technol 53, 337–347 (2016). https://doi.org/10.1007/s13197-015-2037-3

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  • DOI: https://doi.org/10.1007/s13197-015-2037-3

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