Abstract
Fat-soluble nutraceuticals of cereals are known for number of disease preventive activities. Hence wheat bran oil (WBO) and wheat germ oil (WGO) were extracted from wheat bran and germ which yielded 3.35 % and 7.35 % of oil, containing polyunsaturated fatty acids (PUFA) (64 %, 61.2 %) respectively. Both oils contained tocopherols and carotenoids, which were higher in wheat germ oil (273 mg/100 g, 12.23 mg/100 g) than wheat bran oil (190 mg/100 g, 2.21 mg/100 g). Steryl ferulates were also present in both the oils, but their content was eight-fold higher in WBO than in WGO. Three major steryl ferulates identified by HPLC were campesteryl ferulate and sitostenyl ferulate, campestanyl ferulate and β-sitosteryl ferulate as in γ-oryzanol and another ferulate, viz., sitostanyl ferulate. A strong IC50 value of 7.5 mg/mL and 21.6 mg/mL DPPH free radicals scavenging for wheat germ oil for wheat bran oil was observed. NMR (13C and 1H) profile explored the evidence of distribution of antioxidant molecules in the unsaponifiable matter of wheat derived oil. Since oils rich in PUFA and minor components are required for the normal physiological activities, blending such oils with other edible oils of the diet in wheat growing countries like India may be useful to provide health benefits.
Similar content being viewed by others
References
Agudo A, Amiano P, Barcos A, Barricarte A, Beguiristain JM, Chirlaque MD, Dorronsoro M, Gonzalez CA, Lasheras C, Martinez C, Navarro C, Pera G, Quiros JR, Rodriguez M, Tormo MJ (1999) Dietary intake of vegetables and fruits among adults in five regions of Spain. EPIC Group of Spain. European Prospective Investigation into Cancer and Nutrition. Eur J Clin Nutr 53:74–80
Anon (1959) Analytical methods committee- report prepared by the vitamin E panel-the determination of tocopherols in oils, foods and feeding stuffs. Anal Methods Comm Anal (London) 84:356–372
Appett G (1986) Nutritional value and stability of extruded wheat germ product. Getreide, Mehl und Brot 40:176–180
Aruna G, Mamatha BS, Baskaran V (2009) Lutein content of selected Indian vegetables and vegetable oils determined by HPLC. J Food Compos Anal 22:632–636
Cunnane SC, Anderson MJ (1997) Pure linoleate deficiency in the rat: influence on growth, accumulation of n-6 polyunsaturates, and [1-14C] linoleate oxidation. J Lipid Res 38:805–812
Espin JC, Soler-Rivas C, Wichers HJ (2000) Characterization of the total free radical scavenger capacity of vegetable oils and oil fractions using 2,2-diphenyl-1-picrylhydrazyl radical. J Agric Food Chem 48:648–656
Firestone D (1998) Official methods and recommended practices of the American Oil Chemists Society, AOCS method no. Ai 2–75, AOCS method no. Ca 5a-40, AOCS method no. Cd 8–53,AOCS method no. Cd 3c-91, AOCS method no. Cd 1–25, AOCS method no. Ca 6a-40, AOCS method no. Ce 1–62, AOCS method no. Ce 5b-89, AOCS method no. Ce 8–89, 5th edn. AOCS press, Champaign
Gopala Krishna AG, Khatoon S, Shiela PM, Sarmandal CV, Indira TN, Mishra A (2001) Effect of refining of crude rice bran oil on the retention of oryzanol in the refined oil. J Am Oil Chem Soc 78:127–131
Go-Woon J, Md. Salim U, Kwon KT, Chun BS (2010) Comparison of supercritical and near-critical carbon dioxide extraction of carotenoid enriched wheat bran oil. Afr J Biotechnol 9:7702–7709
Gray B, Swick J, Ronnenberg AG (2010) Vitamin E and adiponectin: proposed mechanism for vitamin E-induced improvement in insulin sensitivity. Nutr Res 69:155–161
Hakala P, Maija Lampi A, Ollilainen V, Werner U, Murkovic M, Wahala K, Karkola S, Piironen V (2002) Steryl phenolic acid esters in cereals and their milling fractions. J Agric Food Chem 50:5300–5307
Hassanein MM, Abedel-Razek AG (2009) Chromatographic quantitation of some bioactive minor components in oils of wheat germ and grape seeds produced as by-products. J Oleo Sci 58:227–333
Hu FB, Stampfer MJ, Manson JE, Rimm EB, Colditz GA, Rosner BA, Speizer FE, Hennekens CH, Willett WC (1998) Frequent nut consumption and risk of coronary heart disease in women: prospective cohort study. Br Med J 317:1341–1345
Iwatsuki K, Akihisa T, Tokuda H, Ukiya M, Higashihara H, Mukainaka T, Iizuka M, Hayashi Y, Kimura Y, Nishino H (2003) Sterol ferulates, sterols, and 5-alk (en) cylresorcinols from wheat, rye, and corn bran oils and their inhibitory effects on Epstein-Barr virus activation. J Agric Food Chem 51:6683–6688
Kwon KT, Md. Salim U, Jung GW, Sim JE, Chun BS (2010) Supercritical carbon dioxide extraction of phenolics and tocopherols enriched oil from wheat bran. Int J Biol Sci 6:117–122
Li F, Tan W, Kang Z, Wong CW (2010) Tocotrienol enriched palm oil prevents atherosclerosis through modulating the activities of peroxisome proliferators-activated receptors. Atherosclerosis 211:278–282
Myoung JS, Catherine W, Rico NSH, Kang MY (2011) Effect of oryzanol and ferulic acid on the glucose metabolism of mice fed with a high-fat diet. J Food Sci 76:7–10
Nilufer GN, Suzan K, Yener ME (2009) Optimization of supercritical carbon dioxide extraction of antioxidants from roasted wheat germ based on yield, total phenolic and tocopherol contents, and antioxidant activities of the extracts. J Supercrit Fluids 48:217–224
Nystrom L, Makinen M, Lampi AM, Piironen V (2005) Antioxidant activity of steryl ferulate extracts from rye and wheat bran. J Agric Food Chem 53:2503–2510
Olmedilla B, Granado F, Blanco I, Vaquero M, Cagigal C (2001) Lutein in patients with cataracts and age-related macular degeneration: a long-term supplementation study. J Sci Food Agric 81:904–909
Panfili G, Cinquanta L, Fratianni A, Cubadda R (2003) Extraction of wheat germ oil by supercritical CO2: oil and defatted cake characterization. J Am Oil Chem Soc 80:157–161
Ramadan MF, Afify Amer MM, Sulieman Abd El-Rahman M (2006) Correlation between physicochemical analysis and radical scavenging activity of vegetable oil blends as affected by frying of French fries. Eur J Lipid Sci Technol 108:670–678
Reddy BS, Hirose Y,, Cohen LA, Simi B, Cooma I, Rao CV (2000) Preventive potential of wheat bran fractions against experimental colon carcinogenesis: implications for human colon cancer prevention. Cancer Res 60:4792–4797
Theriault A, Chao JT, Wang Q, Gapor A, Adeli K (1999) Tocotrienols: a review of its therapeutic potential. Clin Biochem 32:309–319
Wilson TA, Nicolosi RJ, Woolfrey B, Kritchevsky D (2007) Rice bran oil and oryzanol reduce plasma lipid and lipoprotein cholesterol concentrations and aortic cholesterol ester accumulation to a greater extent than ferulic acid in hypocholesterolemic hamsters. J Nutr Biochem 18:105–112
Yang CS, Lu G, Ju J, Li GX (2011) Inhibition of inflammation and carcinogenesis in the lung and colon by tocopherols. Ann N Y Acad Sci 1203:29–34
Yasukawa K, Akihisa T, Kimura Y, Tamura T, Takido M (1998) Inhibitory effect of cycloartenyl ferulate, a component of rice bran, on tumor promotion carcinogenesis in mouse skin. Biol Pharm Bull 21:1072–1076
Yongzhi J, Wang T (2005) Phytosterols in cereal by-products. J Am Oil Chem Soc 82:439–444
Acknowledgments
The authors express their thanks to Mrs. TN Indira and Mr. Lokesh, Dept. PCT and Dr. Nanishankar V. Harohally, Dept. FSAQCL, CFTRI, Mysore who helped in the extraction of oil and interpretation of NMR spectra respectively.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kumar, G.S., Krishna, A.G.G. Studies on the nutraceuticals composition of wheat derived oils wheat bran oil and wheat germ oil. J Food Sci Technol 52, 1145–1151 (2015). https://doi.org/10.1007/s13197-013-1119-3
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13197-013-1119-3