Skip to main content
Log in

Effect of processing techniques on nutritional composition and antioxidant activity of fenugreek (Trigonella foenum-graecum) seed flour

  • Original Article
  • Published:
Journal of Food Science and Technology Aims and scope Submit manuscript

Abstract

Fenugreek (Pusa Early Bunching) seeds were processed by using different processing methods viz. soaking, germination and roasting. Raw and processed fenugreek seed flours were analyzed for nutritional composition, anti- nutritional, and antioxidant activity. Raw fenugreek seed flour contained higher amount of dietary fiber (45.4 %) followed by 41.7 % in soaked seed flour, 40.9 % in roasted fenugreek seed flour and 31.3 % in germinated fenugreek seed flour. Processing of fenugreek seeds improved in vitro starch digestibility and in vitro protein digestibility. Soaking, germination and roasting enhanced total phenolic content and the antioxidant activity of fenugreek seed flour as compared to raw fenugreek seed flour. The phenolic content of soaked, germinated and roasted fenugreek seed flours was 54.4, 80.8 and 48.5 mg of gallic acid equivalents/g of sample in contrast to raw fenugreek seed flour (45.4 mg of gallic acid equivalents/g of sample). The antioxidant activity of the extracts of soaked, germinated and roasted fenugreek seed flours was 60.7 %, 73.9 % and 32.0 % whereas as the raw fenugreek seed flour exhibited 18.1 % antioxidant activity. Processing of fenugreek seeds also decreased phytic acid content significantly (P < 0.05) as compared to raw seeds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • El-Aal (1986) Changes in gross chemical composition with emphasis on lipid and protein fractions during germination of fenugreek seeds. Food Chem 22:193–207

    Article  Google Scholar 

  • Ahmad Rafik E-M, Laila AES (1982) Changes in phytate and minerals during germination and cooking of fenugreek seeds. Food Chem 9:149–158

    Article  Google Scholar 

  • Akeson WE, Stahmann MA (1964) A pepsin- pancreatin digestibility index of protein quality evaluation. J Nutr 83:257–259

    CAS  Google Scholar 

  • Anonymous (2012) Sprouting. http://en.wikipedia.org/wiki/Sprouting. Accessed 29 May 2012

  • AOAC (1995) Official methods of analysis of the association of official analytical chemist. Association of Official Analytical Chemist, Washington, DC

    Google Scholar 

  • Asp NG, Johnos CG, Hallmer H, Silijestrom M (1983) Rapid enzymatic assay of insoluble and soluble dietary fiber. J Agric Food Chem 31:476–482

    Article  CAS  Google Scholar 

  • Brand-Williams W, Cuvelier ME, Berset C (1995) Use of free radical method to evaluate antioxidant activity. Lebensm-Wiss u – Technol 28:25–30

    Article  CAS  Google Scholar 

  • Bukhari SB, Bhanger MI, Shahabuddin M (2008) Antioxidant activity of extracts from fenugreek seeds. Pak J Anal Environ Chem 9:78–83

    CAS  Google Scholar 

  • Cevallos-Casals BA, Cisneros-Zevallos L (2010) Impact of germination on phenolic content and antioxidant activity of 13 edible seed species. Food Chem 119:1485–1490

    Article  CAS  Google Scholar 

  • Chandler SF, Dodds JH (1983) The effect of phosphate nitrogen and sucrose on the production of phenolics and socosidine in callus cultures of Solanum tuberosum. Pt Cell Rep 2:105–108

    Article  Google Scholar 

  • Duenas M, Hernandez T, Estrella I, Farnandez D (2009) Germination as process to increase the polyphenol content and antioxidant activity of lupin seeds. Food Chem 117:599–607

    Article  CAS  Google Scholar 

  • Duhan A, Khetarpaul N, Bishnoi S (2002) Content of phytic acid and HCL- extractability of calcium, phosphorus and iron as affected by various domestic processing and cooking methods. Food Chem 78:9–14

    Article  CAS  Google Scholar 

  • El- Mahdy A, El- Sebaiy A (1982) Changes in phytate and minerals during germination and cooking of fenugreek seeds. Food Chem 9:149–158

    Article  CAS  Google Scholar 

  • El-Shimi NM, Damir AA, Ragab M (1984) Changes in some nutrients of fenugreek seeds during germination. Food Chem 14:11–19

    Article  CAS  Google Scholar 

  • Gopalan C, Ramashastri BV, Balasubramanium SC (2004) Nutritive Value of Indian foods. Revised and updated by Narsinga Rao BS, Deosthale YG, Pant KC, Rev edn. National Institute of Nutrition. ICMR, Hyderabad

  • Gupta D, Raju J, Rao AR, Yadava PK, Baquer NZ (2001) T. foenum-graecum seed powder improves glucose homeostasis in alloxan diabetic rat tissues by reversing the altered glycolytic, gluconeogenic and lipogenic enzymes. Mol Cell Biochem 224:45–51

    Article  Google Scholar 

  • Haug W, Lantzsch HJ (1983) Sensitive method for the rapid determination of phytate in cereals and cereal products. J Sci Food Agric 34:11423–11426

    Article  Google Scholar 

  • Hooda S, Jood S (2003) Effect of soaking and germination on nutrient and anti-nutrient contents of fenugreek (Trigonella foenum graecum). J Food Biochem 27:165–176

    Article  CAS  Google Scholar 

  • Jeong SM, Kim SY, Kim DR, Nam KC, Ahn DU, Lee SC (2004) Effect of seed roasting conditions on the antioxidant activity of defatted sesame meal extracts. J Food Sci 69:C377–C381

    Article  CAS  Google Scholar 

  • Jood S, Kapoor AC (1988) Improvement in chickpea and blackpea through processing and cooking techniques. Int J Food Sci Technol 48:307–312

    Google Scholar 

  • Khosla P, Gupta DD, Nagpal RK (1995) Effect of Fenugreek on serum lipids in normal and diabetic rats. Int J Pharmacol 27:89–93

    Google Scholar 

  • Lestienne I, Christele IV, Claire M (2005) Effect of soaking whole and legume seeds on iron, zinc and phytate contents. Food Chem 89:421–425

    Article  CAS  Google Scholar 

  • Mansour EH, EL-Adway (1994) Nutritional potential and functional properties of heat treated and germinated fenugreek seeds. Lebensmittel Wissenschaft Technol 27:568–572

    Article  CAS  Google Scholar 

  • Mathur P, Chaudhary M (2009) Effect of domestic processing on proximate composition of fenugreek seeds. J Food Sci Technol 46:255–258

    CAS  Google Scholar 

  • Naidu MM, Shyamala BN, Naik JP (2011) Chemical composition and antioxidant activity of the husk and endosperm of fenugreek seeds. LWT- Food Sci Technol 44:451–456

    Article  Google Scholar 

  • Neeraja A, Rajyalakshmi P (1996) Hypoglycaemic effect of processed fenugreek seeds in humans. J Food Sci Technol 46:2337–2342

    Google Scholar 

  • NIN Report (1987) Use of fenugreeks seed powder in management of non insulin dependent diabetes mellitus. National Institute of Nutrition, Indian Council of Medical Research

    Google Scholar 

  • Nolan KB, Duffin PA (1987) Effect of phytate on mineral availability. In vitro studies of Mg, Ca, Fe, Cu and Zn solubilities in the presence of phytate. J Sci Food Agric 40:70–83

    Article  Google Scholar 

  • Raghuramulu N, Nair KM, Kalyanasundaram S (2003) A manual of laboratory techniques. NIN press, National Institute of Nutrition, 176 p

    Google Scholar 

  • Randhir R, Lin YT, Shetty K (2004) Phenolics their antioxidant and antimicrobial activity in dark germinated fenugreek sprouts in response to peptide and phytochemical elicitors. Asia Pac J Clin Nutr 13:295–307

    CAS  Google Scholar 

  • Reddy NR, Balakrishnan CV, Salunkhe DK (1978) Phytate phosphorus and mineral changes during germination and cooking of black gram seeds. J Food Sci 43:540–543

    Article  CAS  Google Scholar 

  • Shakuntala S, Jarpala PN, Thangraj J, Madineni MN, Pullabhatla S (2011) Characterization of germinated fenugreek (Trigonella foenum graecum L.) seed fractions. Int J Food Sci 46:2337–2343

    Article  CAS  Google Scholar 

  • Sharma RD (1986) Effect of fenugreek seeds and leaves on blood glucose and serum insulin responses in human subjects. Nutr Res 6:1353–1364

    Article  Google Scholar 

  • Shetty K, Curtis OF, Levin RE, Withowsky R, Ang W (1995) Prevention of vitrification associated with in vitro shoot cultures of oregano (Origanum vulgare) by Pseudomonas spp.. J Plant Physiol 147:447–451

    Article  CAS  Google Scholar 

  • Singh U, Khedekar MS, Jambunathan R (1982) Studies on desi and kabuli chickpea cultivars. The levels of amylase inhibitors, levels of oligosaccharides and in vitro starch digestibility. J Food Sci 47:510–516

    Article  CAS  Google Scholar 

  • Trigg CW (2012) The chemistry of the coffee bean. http://www.web-books.com/Classics/ON/B0/B701/22MB701.html. Accessed 15 Oct 2012

  • Uhl SR (2000) Handbook of spices, seasonings and flavourings. Technomic Publ Co Inc, Lancaster, pp 106–108

    Book  Google Scholar 

  • Zhang D, Hamauzu Y (2004) Phenolics, ascorbic acid, carotenoids and antioxidant activity of broccoli and their changes during conventional and microwave cooking. Food Chem 88:503–509

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The financial support of Senior Research Fellowship provided by Indian Council of Agricultural Research, New Delhi is gratefully acknowledged.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Hemlata Pandey.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pandey, H., Awasthi, P. Effect of processing techniques on nutritional composition and antioxidant activity of fenugreek (Trigonella foenum-graecum) seed flour. J Food Sci Technol 52, 1054–1060 (2015). https://doi.org/10.1007/s13197-013-1057-0

Download citation

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13197-013-1057-0

Keywords

Navigation