Abstract
The present study was conducted to investigate the impact of various treatments of xylanase produced by Aspergillus niger applied in bread making processes like during tempering of wheat kernels and dough mixing on the dough quality characteristics i.e. dryness, stiffness, elasticity, extensibility, coherency and bread quality parameters i.e. volume, specific volume, density, moisture retention and sensory attributes. Different doses (200, 400, 600, 800 and 1,000 IU) of purified enzyme were applied to 1 kg of wheat grains during tempering and 1 kg of flour (straight grade flour) during mixing of dough in parallel. The samples of wheat kernels were agitated at different intervals for uniformity in tempering. After milling and dough making of both types of flour (having enzyme treatment during tempering and flour mixing) showed improved dough characteristics but the improvement was more prominent in the samples receiving enzyme treatment during tempering. Moreover, xylanase decreased dryness and stiffness of the dough whereas, resulted in increased elasticity, extensibility and coherency and increase in volume & decrease in bread density. Xylanase treatments also resulted in higher moisture retention and improvement of sensory attributes of bread. From the results, it is concluded that dough characteristics and bread quality improved significantly in response to enzyme treatments during tempering as compared to application during mixing.
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References
AACC (2000) Approved Methods of American Association of Cereal Chemists. The American Association of Cereal Chemists. Inc, St Paul Minnesota
Ahmad Z (2009) Production and characterization of xylanase for utilization in baking industry. PhD thesis, University of Agriculture, Faisalabad, Pakistan. (http://www.prr.hec.gov.pk/Thesis/158S.pdf, 08.04.2012)
Bajpai P (1999) Application of enzymes in the pulp and paper industry. Biotechnol Progr 15:147–157
Butt MS, Tahir-Nadeem M, Ahmad Z, Sultan MT (2008) Xylanases and their applications in baking industry. Food Tech Biotec 46(1):22–31
Camacho NA, Aguilar GO (2003) Production, purification and characterization of low molecular mass xylanase from Aspergillus sp. and its application in baking. Appl Biochem Biotech 104:159–172
Chithra M, Muralikrishna G (2008) An improved method for obtaining xylanase from finger millet (Eleusine coracana var. ‘Indaf-15’) malt. J Food Sci Tech 45(2):166–169
Collins T, Gerday C, Feller G (2005) Xylanases, xylanase families and extremophilic xylanases. FEMS Microbiol Rev 29:3–23
Courtin CW, Delcour JA (2002) Arabinoxylans and endoxylanases in wheat flour bread-making. J Cereal Sci 35:225–243
Ghildyal NP, Prema P, Srikanta S, Sreekantiah KR, Ahmed SY (1980) Studies on the production of α-amylase in submerged culture. J Food Sci Tech 17(4):165–167
Haros M, Rosell CM, Benedito C (2002) Effect of different carbohydrases on fresh bread texture and bread staling. Eur Food Res Technol 215:425–430
Hilhorst R, Dunnewind B, Orsel R, Stegeman P, van Vliet T, Gruppen H, Schols HA (1999) Baking performance, rheology, and chemical composition of wheat dough and gluten affected by xylanase and oxidative enzymes. J Food Sci 64:808–813
Jaddou H, Mhaisen MT, Al-Hakim M, Zeki L, Al-Ambaky MSH (1986) Semi-pilot production of sucrose from dates and sweet sorghum using ethanolic extraction technique. J Food Sci Tech 23(4):241–243
Jiang Z, Li X, Yang S, Li L, Tan S (2005) Improvement of the breadmaking quality of wheat flour by the hyperthermophilic xylanase B from Thermotoga maritime. Food Res Int 38:37–43
Katina K, Salmenkallio-Marttila M, Partanen R, Forssell P, Autio K (2006) Effects of sourdough and enzymes on staling of high-fibre wheat bread. Lebensm Wiss Technol 39:479–491
Keskin SO, Sumnu G, Sahin S (2004) Usage of enzymes in a novel baking process. Food Nahrung 48:156–160
Kulkarni N, Shendye A, Rao M (1999) Molecular and biotechnological aspects of xylanases. FEMS Microbiol Rev 23:411–456
Laurikainen T, Härkönen H, Autiom K, Poutanen K (1998) Effects of enzymes in fiber-enriched baking. J Sci Food Agric 76:239–249
Leisola M, Jokela J, Pastinen O, Turunen O, Schoemaker H (2002) Industrial use of enzymes. In: Encyclopedia of Life Support Systems (EOLSS), EOLSS Publishers Co, Oxford UK
Maat J, Roza M, Verbakel J, Stam H, MJ Santos da Silva, Bosse M, Egmond MR, Hagemans MLD, Gorcom RFM, Hessing JGM, van den CAMJJ Hondel, van Rotterdam C (1992) Xylanases and their applications in bakery. In: MA Kusters van Someren, Beldman G, Voragen AGJ (eds) Xylan and Xylanases, progress in biotechnology No. 7. VISSER J. Elsevier Science Publishers, Amsterdam, pp 349–360
Maninder K, Bains GS (1976) Effects of amylase supplements on the rheological baking quality of Indian wheats. J Food Sci Tech 13(6):328
Martínez-Anaya MA, Jimenez T (1997) Functionality of enzymes that hydrolyse starch and non-starch polysaccharide in bread making. Eur Food Res Tech 205(3):209–214
Mathewson PR (2000) Enzymatic activity during bread baking. Cereal Foods World 45:98–101
Meilgaard D, Civille GV, Carr BT (1999) Sensory evaluation techniques, 2nd edn. CRC, Boca Raton
Olse HS (1995) Use of enzymes in food processing. In: Reed G, Nagodawithana T (eds) Enzymes, biomass, food and feed biotechnology. Wiley-VCH, Weinheim, pp 663–736
Omar AW, Khataibeh MH, Abu-alruz K (2008) The use of xylanases from different microbial origin in bread making and their effects on bread quality. J Appl Sci 8(4):672–676
Rao BAS, Narasimham VVL (1976) Brewing with enzymes. J Food Sci Tech 13(3):119–123
Romanowska I, Polak J, Janowska K, Bieleckim S (2003) The application of fungal endoxylanase in breadmaking. Comm Agr Appl Biol Sci 68:317–320
Sorensen JF, Sibbesen O, Poulsen CH (2001) Degree of inhibition by the endogenous wheat xylanase inhibitor controls the functionality of microbial xylanases ‘in Dough’. AACC Annual Meeting, Enzymes and Baking – 213AB, Charlotte, NC, USA
Steel RGD, Torrie JH, Dickey DA (1997) Principles and procedures of statistics: a biometric approach. McGraw Hill Book Inc, New York
Subramaniyan S, Prema P (1998) Optimization of cultural parameters for the synthesis of endoxylanase from Bacillus SSP- 34. J Sci Ind Res 57:611–616
Vijaya H, Joseph R (1979) Xylanase production by ultraviolet induced variants of Streptomyces fradiae SCF-5. J Food Sci Tech 15(5):243–246
Vijayanand P, Kulkarni SG, Prathibha GV (2010) Effect of pectinase treatment and concentration of litchi juice on quality characteristics of litchi juice. J Food Sci Tech 47(2):235–239
Wang M, van Vliet T, Hamer RT (2004) Evidence that pentosans/xylanase affects the re-agglomeration of the gluten network. J Cereal Sci 39:341–349
Acknowledgment
The authors are greatly obliged to the National Institute of Food Science and Technology (NIFSAT), University of Agriculture Faisalabad, for providing the facilities to the successful completion of this project.
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Ahmad, Z., Butt, M.S., Ahmed, A. et al. Effect of Aspergillus niger xylanase on dough characteristics and bread quality attributes. J Food Sci Technol 51, 2445–2453 (2014). https://doi.org/10.1007/s13197-012-0734-8
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DOI: https://doi.org/10.1007/s13197-012-0734-8