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Synbiotic yogurt-ice cream produced via incorporation of microencapsulated lactobacillus acidophilus (la-5) and fructooligosaccharide

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Abstract

Yogurt-ice cream is a nutritious product with a refreshing taste and durability profoundly longer than that of yogurt. The probiotic Lactobacillus acidophilus (La-5) cells either in free or encapsulated form were incorporated into yog-ice cream and their survivability were studied. Fructooligosaccharide (FOS) as a prebiotic compound at three levels (0, 4 & 8 % w/w) was added to yogurt-ice cream mix and its effects on some chemical properties, overrun and firmness of product were evaluated. The higher the incorporated FOS concentration, the lower were the pH value and higher the total solid content of treatments. FOS incorporation (8 %) significantly increased the overrun of treatments and reduced their firmness. The viable counts of free probiotics decreased from ~9.55 to ~7.3 log cfu/g after 60 days of frozen storage while that of encapsulated cells merely decreased less than 1 log cycle. Encapsulation with alginate microbeads protected the probiotic cells against injuries in the freezing stage as well as, during frozen storage.

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References

  • Akalin AS, Erisir D (2008) Effect of inulin and oligofructose on the rheological characteristics and probiotic culture survival in low-fat probiotic ice cream. J Food Sci 73:184–188

    Google Scholar 

  • Akin MB, Akin MS, Kirmaci Z (2007) Effects of inulin and sugar levels on the viability of yogurt and probiotic bacteria and the physical and sensory characteristics in probiotic ice-cream. Food Chem 104:93–99

    CAS  Google Scholar 

  • Allan-Wojtas P, Truelstrup HL, Paulson AT (2008) Microstructural studies of probiotic bacteria-loaded alginate microcapsules using standard electron microscopy techniques and anhydrous fixation. LWT-Food Sci Technol 41:101–108

    CAS  Google Scholar 

  • Association of Official Analytical Chemists (2005) Official methods of analysis of AOAC International (18th ed) Maryland, USA.

  • Aziznia S, Khosrowshahi A, Madadlou A, Rahimi J (2008) Whey Protein concentrate and gum tragacanth as fat replacers in nonfat yogurt: chemical, physical, and microstructural properties. J Dairy Sci 91:2545–2552

    CAS  Google Scholar 

  • Azorin-ortuno M, Urban C, Ceron J, Tecles F, Allende A, Tomas-Barberan F, Espin J (2009) Effect of low inulin doses with different polymerization degree on lipid metabolism, mineral absorption, and intestinal microbiota in rats with fat-supplemented diet. Food Chem 113:1058–1065

    CAS  Google Scholar 

  • Baig MI, Prasad V (1995) Biochemical characteristics and stability of Bifidobacterium bifidum in frozen yogurt supplemented with condensed cheese whey. J Dairy Foods Home Sci 15:99–108

    Google Scholar 

  • Childs J (1993) An identity for frozen yoghurt. Cultured Dairy Products J 29:23–24

    Google Scholar 

  • Davidson RH, Duncan SE, Hackney CR, Eigel WN, Boling JW (1999) Probiotic culture survival and implications in fermented frozen yogurt characteristics. J Dairy Sci 83:666–673

    Google Scholar 

  • Dhewa T, Pant S, Mishra V (2012) Development of freeze dried synbiotic formulation using a probiotic strain of Lactobacillus plantarum. J Food Sci Technol. doi:10.1007/s13197-011-0457-2

  • Donkor ON, Nilmini SLI, Stolic P, Vasiljevic T, Shah NP (2007) Survival and activity of selected probiotic organisms in set-Type yogurt during cold storage. Int Dairy J 17:657–665

    CAS  Google Scholar 

  • Emam-Djome Z, Ebrahimzadeh-mousavi M, Gorbani AV, Madadlou A (2008) Effect of whey protein concentrate addition on the physical properties of homogenized sweetened dairy creams. Int J Dairy Technol 61:183–191

    CAS  Google Scholar 

  • Goff HD (1997) Instability and Partial Coalescence in Whippable Dairy Emulsions. J Dairy Sci 80:2620–2630

    CAS  Google Scholar 

  • Guven M, Karaca OB (2002) The effects of varying sugar content and fruit concentration on the physical properties of vanilla and fruit ice cream type frozen yogurts. Int J Dairy Technol 55:27–31

    CAS  Google Scholar 

  • Haynes IN, Playne MJ (2002) Survival of probiotic cultures in low fat ice cream. Aust J Dairy Technol 57:10–14

    Google Scholar 

  • Hekmat S, Mcmahon DJ (1992) Survival of Lactobacillus acidophilus and Bifidobacterium bifidum in ice cream for use as a probiotic food. J Dairy Sci 75:1415–1422

    CAS  Google Scholar 

  • Heydari S, Mortazavian AM, Ehsani MR, Mohammadifar MA, Ezzatpanah H, Sohrabvandi S (2012) Biochemical, microbiological and sensory characteristics of probiotic yogurt containing various prebiotic or fiber compounds. Ital J Food Sci 23:153–163

    Google Scholar 

  • Homayouni A, Azizi A, Ehsani MR, Yarmand MS, Razavi SH (2008) Effect of microencapsulation and resistant starch on the probiotic survival and sensory properties of synbiotic ice cream. Food Chem 111:50–55

    CAS  Google Scholar 

  • Homayouni A, Ehsani MR, Azizi A, Yarmand MS, Razavi SH (2007) Effect of lecithin and calcium chloride solution on the microencapsulation process yield of calcium alginate beads. Iranian Polym J 16:597–606

    CAS  Google Scholar 

  • IDF (1991). Yogurt: determination of titratable acidity- potentiometric method. International Dairy Federation Standard, 150. Brussels – Belgium.

  • Kailasapathy K, Rybka S (1997) Lactobacillus acidophilus and Bifidobacterium spp. Their therapeutic potential and survival in yogurt. Aust J Dairy Technol 52:28–33

    Google Scholar 

  • Karaca OB, Guven M, Yasar K, Kaya S, Kahyaoglu T (2009) The functional, rheological and sensory characteristics of ice creams with various fat replacers. Int J Dairy Technol 62:93–99

    CAS  Google Scholar 

  • Keeney PG, Maga JA (1965) Factors affecting composition of a foam yield of a foam fraction recovered from ice cream. J Dairy Sci 48:1591–1596

    Google Scholar 

  • Krasaekoopt W, Bhandari B, Deeth H (2003) Evaluation of encapsulation techniques of probiotics for yogurt. Int Dairy J 13:3–13

    CAS  Google Scholar 

  • Lyer BK, Singhal RS, Ananthanaryan L (2011) Characterization and in vitro probiotic evaluation of lactic acid bacteria isolated from idli batter. J Food Sci Technol. doi:10.1007/s13197-011-0445-6

  • Mohammadi R, Mortazavian AM (2010) Technological aspects of prebiotics in probiotic fermented milks. Food Rev Int 27:192–212

    Google Scholar 

  • Mohammadi R, Mortazavian AM, Khosrokhavar R, da Cruz AG (2011) Probiotic ice cream: viability of probiotic bacteria and sensory properties. Ann Microbiol 61(3):411–424

    Google Scholar 

  • Mortazavian AM, Ehsani MR, Reinheimer J, Sohrabvandi S (2007a) MRS-bile agar: its suitability for enumeration of mixed probiotic cultures in cultured dairy products. Milchwissenschaft 62:270–272

    CAS  Google Scholar 

  • Mortazavian AM, Razavi SH, Ehsani MR, Sohrabvandi S (2007b) A review: Principles and methods of microencapsulation of probiotics. Iran J Biotechnol 5:1–18

    CAS  Google Scholar 

  • Nagar GE, Clowes G, Tudorica CM, Kuri V, Brennan CS (2002) Rheological quality and stability of yog-ice cream with added inulin. Int J Dairy Technol 55:89–93

    Google Scholar 

  • Nakasawa Y, Hosono A (1992) Functions of fermented milk, challenges for the health science. Elsevier Applied Sci, London, UK

    Google Scholar 

  • Needs EC, Huitson A (1991) The contribution of milk serum proteins to the development of whipped cream structure. Food Struct 10:353–360

    CAS  Google Scholar 

  • Ozer B, Robinson RK, Hrandison AS, Bell AE (1998) Gelation properties of milk concentrated by different techniques. Int Dairy J 8:793–799

    Google Scholar 

  • Shah NP, Ravula RR (2000) Microencapsulation of probiotic bacteria and their survival in frozen fermented dairy desserts. Aust J Dairy Technol 55:139–144

    Google Scholar 

  • Sheu TY, Marshall RT (1993) Microencapsulation of lactobacilli in calcium alginate gels. J Food Sci 54:557–561

    Google Scholar 

  • Sofjan RP, Hartel RW (2004) Effects of overrun on structural and physical characteristics of ice cream. Int Dairy J 14:255–262

    Google Scholar 

  • Sohrabvandi S, Mortazavian AM, Dolatkhah-nejad MR, Bhadori Monfared A (2012) Suitability of MRS-bile agar for the selective enumeration of mixed probiotic bacteria in presence of mesophilic lactic acid culture and yogurt bacteria. Iran J Biotechnol 10:16–21

    Google Scholar 

  • Soukoulis C, Chandrinos I, Tzia C (2008) Study of the functionality of selected hydrocolloids and their blends with k-carrageenan on storage quality of vanilla ice cream. LWT-Food Sci Technol 41:1816–1827

    CAS  Google Scholar 

  • Soukoulis C, Tzia C (2008) Impact of the acidification process, hydrocolloids and protein fortifiers on physical and sensory properties of frozen yogurt. Int J Dairy Technol 61:170–177

    Google Scholar 

  • Vanaja G, Gotcheva V, Angelov A, Agrawal R (2011) Formation of volatiles and fattyacids of therapeutic importance in the probiotic Lactobacillus plantarum LPcfr adapted to resist GIT conditions. J Food Sci Technol 48:110–113

    CAS  Google Scholar 

  • Vinderola CG, Bailo N, Reinheimer JA (2000) Survival of probiotic microfelora in Argentinian yogurts during refrigerated storage. Food Res Int 33:97–102

    CAS  Google Scholar 

  • Vinderola CG, Reinheimer JA (2000) Enumeration of Lactobacillus casei in the presence of Lactobacillus acidophilus, Bifidobacteria and Lactic starter bacteria in fermented dairy products. Int Dairy J 10:271–275

    Google Scholar 

  • Vinderola CG, Reinheimer JA (1999) Culture media for the enumeration of Bifidobacterium bifidum and Lactobacillus acidophilus in the presence of yogurt bacteria. Int Dairy J 9:497–505

    Google Scholar 

  • Wilbey RA, Cooke T, Dimos G (1997) The effects of solute concentration, overrun and storage on the hardness of ice cream. In: Buchheim W (ed) Ice cream. International Dairy Federation, Germany, pp 186–187

    Google Scholar 

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Acknowledgements

We express our appreciation to Dr. Reza Farahmandfar, (Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University, Mashhad, Iran) for his invaluable helps.

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Correspondence to Ashkan Madadlou.

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Ahmadi, A., Milani, E., Madadlou, A. et al. Synbiotic yogurt-ice cream produced via incorporation of microencapsulated lactobacillus acidophilus (la-5) and fructooligosaccharide. J Food Sci Technol 51, 1568–1574 (2014). https://doi.org/10.1007/s13197-012-0679-y

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