Abstract
Yogurt-ice cream is a nutritious product with a refreshing taste and durability profoundly longer than that of yogurt. The probiotic Lactobacillus acidophilus (La-5) cells either in free or encapsulated form were incorporated into yog-ice cream and their survivability were studied. Fructooligosaccharide (FOS) as a prebiotic compound at three levels (0, 4 & 8 % w/w) was added to yogurt-ice cream mix and its effects on some chemical properties, overrun and firmness of product were evaluated. The higher the incorporated FOS concentration, the lower were the pH value and higher the total solid content of treatments. FOS incorporation (8 %) significantly increased the overrun of treatments and reduced their firmness. The viable counts of free probiotics decreased from ~9.55 to ~7.3 log cfu/g after 60 days of frozen storage while that of encapsulated cells merely decreased less than 1 log cycle. Encapsulation with alginate microbeads protected the probiotic cells against injuries in the freezing stage as well as, during frozen storage.
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References
Akalin AS, Erisir D (2008) Effect of inulin and oligofructose on the rheological characteristics and probiotic culture survival in low-fat probiotic ice cream. J Food Sci 73:184–188
Akin MB, Akin MS, Kirmaci Z (2007) Effects of inulin and sugar levels on the viability of yogurt and probiotic bacteria and the physical and sensory characteristics in probiotic ice-cream. Food Chem 104:93–99
Allan-Wojtas P, Truelstrup HL, Paulson AT (2008) Microstructural studies of probiotic bacteria-loaded alginate microcapsules using standard electron microscopy techniques and anhydrous fixation. LWT-Food Sci Technol 41:101–108
Association of Official Analytical Chemists (2005) Official methods of analysis of AOAC International (18th ed) Maryland, USA.
Aziznia S, Khosrowshahi A, Madadlou A, Rahimi J (2008) Whey Protein concentrate and gum tragacanth as fat replacers in nonfat yogurt: chemical, physical, and microstructural properties. J Dairy Sci 91:2545–2552
Azorin-ortuno M, Urban C, Ceron J, Tecles F, Allende A, Tomas-Barberan F, Espin J (2009) Effect of low inulin doses with different polymerization degree on lipid metabolism, mineral absorption, and intestinal microbiota in rats with fat-supplemented diet. Food Chem 113:1058–1065
Baig MI, Prasad V (1995) Biochemical characteristics and stability of Bifidobacterium bifidum in frozen yogurt supplemented with condensed cheese whey. J Dairy Foods Home Sci 15:99–108
Childs J (1993) An identity for frozen yoghurt. Cultured Dairy Products J 29:23–24
Davidson RH, Duncan SE, Hackney CR, Eigel WN, Boling JW (1999) Probiotic culture survival and implications in fermented frozen yogurt characteristics. J Dairy Sci 83:666–673
Dhewa T, Pant S, Mishra V (2012) Development of freeze dried synbiotic formulation using a probiotic strain of Lactobacillus plantarum. J Food Sci Technol. doi:10.1007/s13197-011-0457-2
Donkor ON, Nilmini SLI, Stolic P, Vasiljevic T, Shah NP (2007) Survival and activity of selected probiotic organisms in set-Type yogurt during cold storage. Int Dairy J 17:657–665
Emam-Djome Z, Ebrahimzadeh-mousavi M, Gorbani AV, Madadlou A (2008) Effect of whey protein concentrate addition on the physical properties of homogenized sweetened dairy creams. Int J Dairy Technol 61:183–191
Goff HD (1997) Instability and Partial Coalescence in Whippable Dairy Emulsions. J Dairy Sci 80:2620–2630
Guven M, Karaca OB (2002) The effects of varying sugar content and fruit concentration on the physical properties of vanilla and fruit ice cream type frozen yogurts. Int J Dairy Technol 55:27–31
Haynes IN, Playne MJ (2002) Survival of probiotic cultures in low fat ice cream. Aust J Dairy Technol 57:10–14
Hekmat S, Mcmahon DJ (1992) Survival of Lactobacillus acidophilus and Bifidobacterium bifidum in ice cream for use as a probiotic food. J Dairy Sci 75:1415–1422
Heydari S, Mortazavian AM, Ehsani MR, Mohammadifar MA, Ezzatpanah H, Sohrabvandi S (2012) Biochemical, microbiological and sensory characteristics of probiotic yogurt containing various prebiotic or fiber compounds. Ital J Food Sci 23:153–163
Homayouni A, Azizi A, Ehsani MR, Yarmand MS, Razavi SH (2008) Effect of microencapsulation and resistant starch on the probiotic survival and sensory properties of synbiotic ice cream. Food Chem 111:50–55
Homayouni A, Ehsani MR, Azizi A, Yarmand MS, Razavi SH (2007) Effect of lecithin and calcium chloride solution on the microencapsulation process yield of calcium alginate beads. Iranian Polym J 16:597–606
IDF (1991). Yogurt: determination of titratable acidity- potentiometric method. International Dairy Federation Standard, 150. Brussels – Belgium.
Kailasapathy K, Rybka S (1997) Lactobacillus acidophilus and Bifidobacterium spp. Their therapeutic potential and survival in yogurt. Aust J Dairy Technol 52:28–33
Karaca OB, Guven M, Yasar K, Kaya S, Kahyaoglu T (2009) The functional, rheological and sensory characteristics of ice creams with various fat replacers. Int J Dairy Technol 62:93–99
Keeney PG, Maga JA (1965) Factors affecting composition of a foam yield of a foam fraction recovered from ice cream. J Dairy Sci 48:1591–1596
Krasaekoopt W, Bhandari B, Deeth H (2003) Evaluation of encapsulation techniques of probiotics for yogurt. Int Dairy J 13:3–13
Lyer BK, Singhal RS, Ananthanaryan L (2011) Characterization and in vitro probiotic evaluation of lactic acid bacteria isolated from idli batter. J Food Sci Technol. doi:10.1007/s13197-011-0445-6
Mohammadi R, Mortazavian AM (2010) Technological aspects of prebiotics in probiotic fermented milks. Food Rev Int 27:192–212
Mohammadi R, Mortazavian AM, Khosrokhavar R, da Cruz AG (2011) Probiotic ice cream: viability of probiotic bacteria and sensory properties. Ann Microbiol 61(3):411–424
Mortazavian AM, Ehsani MR, Reinheimer J, Sohrabvandi S (2007a) MRS-bile agar: its suitability for enumeration of mixed probiotic cultures in cultured dairy products. Milchwissenschaft 62:270–272
Mortazavian AM, Razavi SH, Ehsani MR, Sohrabvandi S (2007b) A review: Principles and methods of microencapsulation of probiotics. Iran J Biotechnol 5:1–18
Nagar GE, Clowes G, Tudorica CM, Kuri V, Brennan CS (2002) Rheological quality and stability of yog-ice cream with added inulin. Int J Dairy Technol 55:89–93
Nakasawa Y, Hosono A (1992) Functions of fermented milk, challenges for the health science. Elsevier Applied Sci, London, UK
Needs EC, Huitson A (1991) The contribution of milk serum proteins to the development of whipped cream structure. Food Struct 10:353–360
Ozer B, Robinson RK, Hrandison AS, Bell AE (1998) Gelation properties of milk concentrated by different techniques. Int Dairy J 8:793–799
Shah NP, Ravula RR (2000) Microencapsulation of probiotic bacteria and their survival in frozen fermented dairy desserts. Aust J Dairy Technol 55:139–144
Sheu TY, Marshall RT (1993) Microencapsulation of lactobacilli in calcium alginate gels. J Food Sci 54:557–561
Sofjan RP, Hartel RW (2004) Effects of overrun on structural and physical characteristics of ice cream. Int Dairy J 14:255–262
Sohrabvandi S, Mortazavian AM, Dolatkhah-nejad MR, Bhadori Monfared A (2012) Suitability of MRS-bile agar for the selective enumeration of mixed probiotic bacteria in presence of mesophilic lactic acid culture and yogurt bacteria. Iran J Biotechnol 10:16–21
Soukoulis C, Chandrinos I, Tzia C (2008) Study of the functionality of selected hydrocolloids and their blends with k-carrageenan on storage quality of vanilla ice cream. LWT-Food Sci Technol 41:1816–1827
Soukoulis C, Tzia C (2008) Impact of the acidification process, hydrocolloids and protein fortifiers on physical and sensory properties of frozen yogurt. Int J Dairy Technol 61:170–177
Vanaja G, Gotcheva V, Angelov A, Agrawal R (2011) Formation of volatiles and fattyacids of therapeutic importance in the probiotic Lactobacillus plantarum LPcfr adapted to resist GIT conditions. J Food Sci Technol 48:110–113
Vinderola CG, Bailo N, Reinheimer JA (2000) Survival of probiotic microfelora in Argentinian yogurts during refrigerated storage. Food Res Int 33:97–102
Vinderola CG, Reinheimer JA (2000) Enumeration of Lactobacillus casei in the presence of Lactobacillus acidophilus, Bifidobacteria and Lactic starter bacteria in fermented dairy products. Int Dairy J 10:271–275
Vinderola CG, Reinheimer JA (1999) Culture media for the enumeration of Bifidobacterium bifidum and Lactobacillus acidophilus in the presence of yogurt bacteria. Int Dairy J 9:497–505
Wilbey RA, Cooke T, Dimos G (1997) The effects of solute concentration, overrun and storage on the hardness of ice cream. In: Buchheim W (ed) Ice cream. International Dairy Federation, Germany, pp 186–187
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We express our appreciation to Dr. Reza Farahmandfar, (Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University, Mashhad, Iran) for his invaluable helps.
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Ahmadi, A., Milani, E., Madadlou, A. et al. Synbiotic yogurt-ice cream produced via incorporation of microencapsulated lactobacillus acidophilus (la-5) and fructooligosaccharide. J Food Sci Technol 51, 1568–1574 (2014). https://doi.org/10.1007/s13197-012-0679-y
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DOI: https://doi.org/10.1007/s13197-012-0679-y