Abstract
The effect of antifungal hot-water treatments (AHWT) at 55 °C for 0, 3, 6 and 9 min on quality attributes and cell-wall enzymatic activity during storage at 25 °C was investigated in papaya fruit. The total soluble solids (TSS), pH, titratable acidity (TA), firmness and fresh weight loss were not affected, whereas color on skin was negatively affected by the treatments of 6- and 9-min. However, the skin color was not different between the 3-min treated fruit and the untreated fruit during the storage. Decay was delayed and reduced by AHWT. We observed that the 3-min treatment of 55 °C did not affect softening and quality of papaya cv Maradol when applied as a pesticide-free treatment at color-break stage of papaya. PME (Pectinmethylesterase) and PG (Polygalacturonase) activities were not significantly affected by AHWT. We concluded that the AHWT did not affect the softening process from papaya pulp since the cell-wall enzyme activity (PME and PG) was not altered by treatments.
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Adaskaveg JE, Forster H, Sommer NF (2002) Principles of postharvest pathology and management of decays of edible horticultural crops. Ch. 17. In: Kader AA (ed) Postharvest technology of horticultural crops. University of California. Agriculture and Natural Resources. Publication 3311, pp 163–195
Aina JO, Oladunjoye OO (1993) Respiration, pectolytic activity and textural changes in ripening African mango (Irvinga gabonensis) fruits. J Sci Food Agric 63:451–454
Akamine EK, Hundtoft EB (1971) Establishing the effects of post-harvest treatment on fresh market papayas by response surface methodology. J Agric Eng Res 16:343–352
Almeida MEM, Nogueira JN (1995) The control of polyphenol oxidase activity in fruits and vegetables. A study of the interactions between the chemical compounds used and heat treatment. Plant Foods Hum Nutr 47:245–256
AOAC (1998) Official methods of analysis. 16th ed, Association of Official Analytical Chemists. Guithersburg, MD. USA
Awad M, Young RE (1979) Postharvest variation in cellulase, polygalacturonase and pectin methylesterase in avocado (Persea americana Mill., cv. Fuertes) fruits in relation to respiration and ethylene production. Plant Physiol 64:306–308
Barca EA, Kalantari S, Markhlouf J, Arul J (2000) Impact of UV-C irradiation on cell wall degrading enzymes during ripening of tomato (Lycopersicon esculentum L.) fruit. J Agric Food Chem 48:667–671
Bennet AB (2001) Biochemical and genetic determinants of cell wall disassembly in ripening fruit: a general model. HortScience 37:447–450
Cámara MM, Díez C, Torija ME (1993) Changes during ripening of papaya fruit in different storage systems. Food Chem 46:81–84
Cantwell M, Nie X (1996) Use of heat treatments to control postharvest pathogens on tomatoes and melons. In: Organica‘92. Proc. Organic Farming Symp. Univ. California Div. Agr. Natl. Res. Publ. 3356. pp 96–101
Carrillo-López A, Cruz-Hernández A, Cárabez-Trejo A, Guevara-Lara F, Paredes-López O (2002) Hydrolytic activity and ultrastructural changes in fruit skins from two prickly pear (Opuntia sp.) varieties during storage. J Agric Food Chem 50:1681–1685
Carrillo-López A, Cruz-Hernández A, Guevara-Lara F, Paredes-López O (2003) Physicochemical changes during ripening in storage of two varieties of prickly pear stored at 18 °C. J Food Sci Technol 40:461–464
Chan HT Jr, Tam SYT, Seo ST (1981) Papaya polygalacturonase and its role in thermally injured ripening fruit. J Food Sci 46:190–192
Chan HT Jr, Sanxter SS, Nishijima KA (1994) Heat-treating “Sharwil” avocado for cold tolerance in quarantine cold treatments. HortScience 29:1166–1168
Couey HM, Alvarez AM, Nelson MG (1984) Comparison of hot-water spray and inmersion treatments for control of postharvest decay of papaya. Plant Dis 68:436–437
Espinoza-Guevara R, Caro-Corrales J, Ordorica-Falomir C, Zazueta-Morales J, Vega-Garcia M, Cronin K (2010) Thermophysical properties of pulp and rind of papaya Cv Maradol. Int J Food Prop 13:65–74
Fayyaz A, Asbi BA, Ghazali HM, Che Man YB, Jinap S (1993) Pectinesterase extraction from papaya. Food Chem 47:183–185
Gaete-Eastman C, Figueroa CR, Balbontín C, Moya M, Atkinson RG, Herrera R, Moya-León MA (2009) Expression of an ethylene-related expansin gene during softening of mountain papaya fruit (Vasconcellea pubescens). Postharvest Biol Technol 53:58–65
Gross KC (1982) A rapid and sensitive spectrophotometric method for assaying polygalacturonase using 2-cyanoacetamide. HortScience 17:993–994
Hagerman AE, Austin PJ (1986) Continuous spectrophotometric assay for plant pectin methyl esterase. J Agric Food Chem 34:440–444
Huber D (1983) The role of cell wall hydrolases in fruit softening. Hort Rev 5:169–219
Jagtiani J, Chan HT Jr, Sakai WS (1988) Papaya. In: Jagtiani J, Chan HT Jr, Sakai WS (eds) Tropical fruit processing. Academic Press Inc, USA, pp 105–145
Klein JD, Lurie S (1992) Heat treatments for improved postharvest quality of horticultural crops. HortTechnol 2:316–320
Lazan H, Ali ZM, Liang KS, Yee KL (1989) Polygalacturonase activity and variation in ripening of papaya fruit with tissue depth and heat treatment. Physiol Plant 77:93–98
Lurie S (1998) Postharvest heat treatments of horticultural crops. Hort Rev 22:91–121
McDonald RE, McCollum TG, D’Aquino S (1993) Heat treatment inhibits mango chilling injury. HortScience 28:197–198
McDonald RE, McCollum TG, Baldwin EA (1998) Heat treatment of mature-green tomatoes: differential effects of ethylene and partial ripening. J Amer Soc Hort Sci 123:457–462
McGregor BM (1987) Manual de Transporte de Productos Tropicales. Departamento de Agricultura de los Estados Unidos. Manual de Agricultura. Washington, D.C. USA, No. 668. 103 p. (In Spanish)
McGuire RG (1992) Reporting of objective color measurements. HortScience 27:1254–1255
Montgomery DC (1991) Diseño y Análisis de Experimentos. Grupo editorial Iberoamericana, S.A. de C.V. pp 175–222 México, D.F. (In Spanish)
Paull RE (1994) Response of tropical horticultural commodities to insect disinfestation treatments. HortScience 29:988–991
Paull RE, Armstrong JW (1994) Insect pests and fresh horticultural products: Treatments and Responses. 360 p. Wallingford, UK, Cab International
Paull RE, Chen NJ (1990) Heat shock response in field-grown, ripening papaya fruit. J Amer Soc Hort Sci 115:623–631
Perez-Carrillo E, Yahia EM (2004) Effect of postharvest hot air and fungicide treatments on the quality of “Maradol” Papaya (Carica papaya L.). J Food Qual 27:127–139
Pressey R, Avants JK (1982) Solubilization of cell walls by tomato polygalacturonase: effects of pectinesterase. J Food Biochem 6:57–74
Ramakrishna M, Haribabu K, Purushotham K (2002) Rffect of Post-harvest application of growth regulators on storage behavior of papaya (Carica papaya L.) Cv. ‘Co-2’. J Food Sci Technol 39:657–659
Roe B, Bruemmer JH (1981) Changes in pectin substances and enzymes during ripening and storage of “Keitt” mangos. J Food Sci 46:186–189
Salunkhe DK, Desai BB (1984) Postharvest biotechnology of fruits. Ch. 3. Vol. II. CRC Press, Inc, Boca Raton, pp 13–25
SG PLUS (1992) Statgraphics plus version 6.0. Statistical graphics system. Rockville, MA, USA. Statistical Graphics Co
Watada AE, Abe K, Yamuchi N (1990) Physiological activities of partially processed fruits and vegetables. Food Technol 44:116–122
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Chávez-Sánchez, I., Carrillo-López, A., Vega-García, M. et al. The effect of antifungal hot-water treatments on papaya postharvest quality and activity of pectinmethylesterase and polygalacturonase. J Food Sci Technol 50, 101–107 (2013). https://doi.org/10.1007/s13197-011-0228-0
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DOI: https://doi.org/10.1007/s13197-011-0228-0