Skip to main content

Advertisement

Log in

Disinfecting the acrylic resin plate using electrolyzed acid water and 2% glutaraldehyde: a comparative microbiological study

  • Original Article
  • Published:
The Journal of Indian Prosthodontic Society

Abstract

Marked bactericidal activity of the electrolyzed acid water (EAW) and its less undesirable effects on biological tissues and the environment has been successfully utilized in dental practice. A review of literature reveals that not many studies have been performed to determine the disinfection effect of EAW on heat cured acrylic denture bases. The objective of the study is to determine the efficacy of EAW in disinfecting heat cured acrylic resin specimens as compared to the efficacy of disinfecting the specimens using 2% glutaraldehyde. Heat cured acrylic resin plates were immersed in Bacteria suspensions of Staphylococcus aureus and later disinfected using EAW which was produced using a custom made electrolyzing apparatus and 2% glutaraldehyde for different time intervals. The numbers of surviving bacteria were counted. As the control, the bacteria attached on the specimens were counted with out any disinfection treatment. The results obtained were statistically analyzed using ANOVA and post-hoc test. The disinfection potential of 2% glutaraldehyde was better than EAW when the specimens were disinfected for 1 and 3 min. Where as when the disinfection time was increased to 5 min there was no difference between EAW and 2% glutaraldehyde. However considering that the disinfecting time can be easily extended to 5 min, EAW can be effectively used for disinfecting the acrylic denture.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  1. Powell GL, Runnells RD, Saxon BA, Whisenant BK (1990) The presence and identification of organisms to dental laboratories. J Prosthet Dent 64:235

    Article  PubMed  Google Scholar 

  2. ADA Council on Scientific Affairs and ADA Council on Dental Practice (1996) Infection control recommendations for the dental office and dental laboratory. J Am Dent Assoc 127:672–680

    Google Scholar 

  3. Ananthanarayan Paniker CKJ (2000) Text book of microbiology, 6th edn. Orient Longman Publishers, Hyderabad, pp 178–186

    Google Scholar 

  4. Rudd RW, Senia ES, McCleskey FK, Adams ED (1984) Sterilization of complete dentures with sodium hypochlorite. J Prosthet Dent 51(3):318–321

    Article  PubMed  Google Scholar 

  5. Chau VB, Saunders TR, Pimster M, Elfring DR (1995) In depth disinfection of acrylic resins. J Prosthet Dent 74:309–313

    Article  PubMed  Google Scholar 

  6. Shen C, Javid NS, Colazzi FA (1989) The effect of glutaraldehyde disinfectants or denture base resins. J Prosthet Dent 61(5):583–589

    Article  PubMed  Google Scholar 

  7. Bell JA, Brockmann SL, Fel P, Sackuvich DA (1989) The effectiveness of two disinfectants on denture base acrylic resin with an organic load. J Prosthet Dent 61(5):580–583

    Article  PubMed  Google Scholar 

  8. Brace ML, Plummer KD (1993) Practical denture disinfection. J Prosthet Dent 70:538–540

    Article  PubMed  Google Scholar 

  9. UnLu A, Altay OT, Sahmali S (1996) The role of denture cleansers on the whitening of acrylic resins. Int J Prosthodont 9:266–270

    PubMed  Google Scholar 

  10. Ma T, Johnson GH, Gordon GE (1997) Effects of chemical disinfectants on the surface characteristics and color of denture resins. J Prosthet Dent 77(2):197–204

    Article  PubMed  Google Scholar 

  11. Mc Name SJ, Von Gonten AS, woalsey GD (1991) Effects of laboratory disinfecting agents on color stability of denture acrylic resins. J Prosthet Dent 66:132–136

    Article  Google Scholar 

  12. Nikawa H, Iwanaga H, Hamada T, yuhta S (1994) Effects of denture cleansers on direct soft denture lining materials. J Prosthet Dent 72:657–662

    Article  PubMed  Google Scholar 

  13. Ichihara T, Fujii G, Eda T, Sasaki M, Veda Y (2004) The efficacy function water (electrolyzed strong acid solution) on open heart surgery; post operative mediastinitis due to methicillin-resistant Staphylococcus aureus. Kyobu Geka 57(12):110–112

    Google Scholar 

  14. Machado AP, Fischman O, Geocze S (2005) Microbiological evaluation of gastroscope decontamination by electrolyzed acid water. Arq Gastroenterol 45(1):60–62

    Article  Google Scholar 

  15. Lee JH, Rhee PL, Kim JH, Kim JJ, Paik SW, Rhee JC, Song JH, Yeom JS, Lee NY (2004) Efficacy of electrolyzed acid water in reprocessing patient-used flexible upper endoscopes: comparison with 2% alkaline glutaraldehyde. J Gastroenterol Hepatol 19(8):897–903

    Article  PubMed  Google Scholar 

  16. Yahagi N, Kono M, Kitahara M, Ohmura A, Sumita O, Hashimoto T, Hori K, Nig-Juan C, Woodson P, Kubota S, Murakami A, Takamoto S (2000) Effect of electrolyzed water on wound healing. Artif Organs 24(12):984–987

    Article  PubMed  Google Scholar 

  17. Morita C, Sano K, Morimatsu S, Kiura H, Goto T, Kohno T, Hong WU, Miyoshi H, Iwasawa A, Nakamura Y, Tagawa M, Yokosuka O, Saisho H, Maeda T, Katsuoka Y (2000) Disinfection potential of electrolyzed solutions containing sodium chloride at low concentrations. J Viral Methods 85(1–2):163–174

    Article  Google Scholar 

  18. Fabrizio KA, Sharma RR, Demirci A, Cutter CN (2002) Comparison of electrolyzed oxidizing water with various antimicrobial interventions to reduce salmonella species on poultry. Poult Sci 81(10):1598–1605

    PubMed  Google Scholar 

  19. Sharma RR, Demrici A (2003) Treatment of Escherichia coli (0157:H7) inoculated alfalfa seeds and sprouts with electrolyzed oxidizing water. Int J Food Microbial 86(3):231–237

    Article  Google Scholar 

  20. Kim C, Hong YC, Brackett RE (2000) Roles of oxidation–reduction potential in electrolyzed oxidizing and chemically modified water for inactivation of food-related pathogens. J Food Prot 63(1):19–24

    PubMed  Google Scholar 

  21. Horiba N, Hiratsuka K, Onoe T, Yoshida T, Suzuki K, Matsvmoto T, Nakamura H (1999) Bacterial effect of electrolyzed neutral water on bacteria isolated from infected root canals. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 87(1):83–87

    Article  PubMed  Google Scholar 

  22. Ito K, Nishida T, Marai S (1996) Inhibitory effects of acid water prepared by an electrolysis apparatus on early formation on specimens of dentine. J Clin Periodontol 23:471–476

    Article  PubMed  Google Scholar 

  23. Kohono S, Kawata T, Kku M, Fujita T, Tsutsui K, Ohtani J, Tenjo K, Motokawa M, Tohma Y, Shigekawa M, Kamata H, Tanne K (2004) Bactericidal effects of acidic electrolyzed water on the dental unit waterline. Jpn J Infect Dis 57:52–54

    Google Scholar 

  24. Nagamastu Y, Tajima K, Kakigawa H, Kozono Y (2001) Application of electrolyzed acid water on the denture base. Part 1. Examination of sterilization of effects on resin plate. Dent Mater J 20(2):148–155

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to K. R. Jnanadev.

Additional information

This article presented at the 36th IPS conference, Bangalore.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jnanadev, K.R., Satish Babu, C.L., Shilpa Shetty, S. et al. Disinfecting the acrylic resin plate using electrolyzed acid water and 2% glutaraldehyde: a comparative microbiological study. J Indian Prosthodont Soc 11, 36–44 (2011). https://doi.org/10.1007/s13191-011-0057-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13191-011-0057-x

Keywords

Navigation