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Substitution of His260 residue alters the thermostability of Pseudoalteromonas carrageenovora arylsulfatase

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Abstract

This study aimed to improve the thermostability of arylsulfatase from Pseudoalteromonas carrageenovora. A library of P. carrageenovora arylsulfatase mutants was constructed by introducing random mutagenesis using error-prone PCR. After screening, two mutants of H260L and D84A/H260L showed enhanced thermal stability than the wild-type predecessor (WT). Site-directed mutagenesis demonstrated that only amino acid residue at Position 260 plays an important role in the thermostability of P. carrageenovora arylsulfatase. Thermal inactivation analysis showed that the half-life (t1/2) values at 55°C for H260L, H260I, H260Q, H260F and H260R were 40.6, 48.4, 30.9, 29.1 and 34.5 min, respectively, while that of WT was 9.1 min. Structure modeling demonstrated that the additional hydrogen bonds and/or optimization of surface charge-charge interactions could be responsible for the increased thermostability imparted by H260L, H260I, H260Q, H260F and H260R.

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Authors and Affiliations

  1. College of Food and Biological Engineering, Jimei University, Xiamen, 361021, China

    Yanbing Zhu, Xiaoqian Yin, Han Liu, Yanhong Chen, Lijun Li, Anfeng Xiao & Hui Ni

  2. Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen, 361021, China

    Yanbing Zhu, Yanhong Chen, Lijun Li, Anfeng Xiao & Hui Ni

  3. Research Center of Food Biotechnology of Xiamen City, Xiamen, 361021, China

    Yanbing Zhu, Yanhong Chen, Lijun Li, Anfeng Xiao & Hui Ni

  4. Key Laboratory of Systemic Utilization and In-depth Processing of Economic Seaweed, Xiamen Southern Ocean Technology Center of China, Xiamen, 361021, China

    Yanbing Zhu, Yanhong Chen, Lijun Li, Anfeng Xiao & Hui Ni

  5. Department of Pharmacy, Xiamen Medical College, Xiamen, 361023, China

    Hebin Li

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  1. Yanbing Zhu
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Correspondence to Hui Ni.

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Foundation item: The National Natural Science Foundation of China under contract No. 31401632; the Program for New Century Excellent Talents in Fujian Province University, China under contract No. B15139.

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Zhu, Y., Yin, X., Liu, H. et al. Substitution of His260 residue alters the thermostability of Pseudoalteromonas carrageenovora arylsulfatase. Acta Oceanol. Sin. 38, 75–82 (2019). https://doi.org/10.1007/s13131-019-1356-z

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  • DOI: https://doi.org/10.1007/s13131-019-1356-z

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