Abstract
Acute myocardial infarction (AMI) is a common disease with high morbidity and mortality worldwide. However, postinfarction pathogenesis remains unclear, and it is particularly important to identify new therapeutic targets. The RNA-binding motif protein RBM3 (also known as cold-inducible protein) is known to promote translation and is associated with tumor proliferation and neuroprotection. However, little is known about the biological effects of RBM3 on myocardial infarction. In the present study, we found that RBM3 expression was significantly upregulated in ischemia–reperfusion (I/R) condition and downregulation of RBM3 inhibited autophagy and promoted apoptosis in cardiomyocytes. We confirmed that RBM3 interacts with Raptor to regulate the autophagy pathway. Taken together, these findings illustrate the protective effects of RBM3 against I/R-induced myocardial apoptosis through the autophagy pathway.
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Funding
This work was supported in part by the National Science Foundation of China (grant nos. 31760292 and 81460210), the Department project of Science and Technology of Yunnan Province (grant nos. 2017FA035 and 2019ZF011-2), Joint Fund for Basic Research of Yunnan Provincial Department of Science and Technology and Kunming Medical University (grant no. 202101AY070001-079), Kunming Science and Technology Plan Project (grant no. 2019–1-N-25318000003496), and the Department project of Education of Yunnan Province (grant no. 2020Y0119).
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DL and LS conceived and designed the study; LMTW, CYL, PR, and XW performed the experiments; YZX and LY analyzed the data; DL, LS, and NW wrote the manuscript. All authors read and approved the final manuscript. The authors declare that all data were generated in-house and that no paper mill was used.
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Key points
• RBM3 expression is up-regulated under myocardial ischemia–reperfusion conditions.
• RBM3 regulates apoptosis and autophagy in cardiomyocytes.
• RBM3 may interact with Raptor to regulate cardiomyocyte autophagy.
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Wang, N., Wang, L., Li, C. et al. RBM3 interacts with Raptor to regulate autophagy and protect cardiomyocytes from ischemia–reperfusion-induced injury. J Physiol Biochem 79, 47–57 (2023). https://doi.org/10.1007/s13105-022-00919-z
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DOI: https://doi.org/10.1007/s13105-022-00919-z