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Preconditioning with diosgenin and treadmill exercise preserves the cardiac toxicity of isoproterenol in rats

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Abstract

This study was aimed to evaluate the preventive effect of diosgenin and exercise on tissue antioxidant status in isoproterenol-induced myocardial infarction (MI) in male Wistar rats. Levels of lipid peroxides, reduced glutathione (GSH), and the activities of glutathione-dependent antioxidant enzymes (glutathione peroxidise and glutathione reductase) and antiperoxidative enzymes (catalase and superoxide dismutase) in the plasma and the heart tissue of experimental groups of rats were determined. Pretreatment with diosgenin and exercise exerted an antioxidant effect against isoproterenol-induced myocardial infarction by blocking the induction of lipid peroxidation. A tendency to prevent the isoproterenol-induced alterations in the level of GSH, in the activities of glutathione-dependent antioxidant enzymes and antiperoxidative enzymes was also observed. Histopathological findings of the myocardial tissue showed a protective role for combination of diosgenin and exercise in isoproterenol (ISO)-treated rats. Thus, the present study reveals that preconditioning with diosgenin and exercise exerts cardioprotective effect against ISO-induced MI due to its free radical scavenging and antioxidant effects, which maintains the tissue defense system against myocardial damage.

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Acknowledgments

This research was funded by the Applied Drug Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. The authors declare that they have no conflict of interests.

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Authors and Affiliations

  1. Department of Physiology, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Afshin Salimeh & Mustafa Mohammadi

  2. Department of Anatomy and Histology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

    Bahman Rashidi

  3. 108-125 Indian Road, Kitchener, ON, N2B 2S8, Canada

    Afshin Salimeh

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  1. Afshin Salimeh
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Correspondence to Afshin Salimeh.

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Salimeh, A., Mohammadi, M. & Rashidi, B. Preconditioning with diosgenin and treadmill exercise preserves the cardiac toxicity of isoproterenol in rats. J Physiol Biochem 69, 255–265 (2013). https://doi.org/10.1007/s13105-012-0208-5

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