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Vasculopathy in type 2 diabetes mellitus: role of specific angiogenic modulators

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Abstract

Type 2 diabetes mellitus (T2DM) is largely defined by hyperglycemia that promotes vascular complications. Abnormal angiogenesis has been claimed to have a role in this disease. This study aimed to investigate serum levels of both conventional and other markers of angiogenesis not well studied before in diabetes, and to correlate findings with age of the patients, glycemic control, presence of microvascular complications, and oxidative stress. Thirty-eight patients with T2DM and 13 age- and sex-matched healthy persons representing controls were recruited. Serum levels of basic fibroblast growth factor (b-FGF) was measured by immunosorbent assay kit; advanced glycosylation end products, platelet-derived endothelial cell growth factor (PD-ECGF), cathepsin-D (CD), gangliosides, hyaluronic acid (HA), nitric oxide (NO), lipid peroxides (LPER), superoxide dismutase, and total thiols by chemical methods; and copper (Cu) by atomic absorption flame photometry. Advanced glycosylation end products and angiogenic factors (b-FGF, PD-ECGF, CD, gangliosides, HA, and Cu) were significantly higher in patients than controls. Oxidative stress markers, NO, and LPER were significantly higher while total thiols were significantly lower in patients than controls. These changes were more pronounced with age, poor glycemic control, and presence of microvascular complications. Angiogenesis dysfunction coinciding with elevated levels of many angiogenic growth factors may point to their malfunctioning due to oxidative stress and/or protein glycation at the factor and the receptor levels. This necessitates further investigations.

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Conflicts of interest

There are no conflicts of interest.

Limitation of the study

The small number of patients and study being cross-sectional rather than longitudinal are the major limitations of this study. The major cause was the limitation of the local research resources and budget to cover a few hundred patients required for proper sample size and an elaborate longitudinal and may be interference study.

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Authors and Affiliations

  1. Department of Physiology, Assiut University, P.O. Box: 71526, Assiut, Egypt

    Enas A. Hamed & Effat M. Abdel Moneim

  2. Department of Biochemistry, Assiut University, Assiut, Egypt

    Madeha M. Zakary

  3. Department of Internal medicine, Faculty of Medicine, Assiut University, Assiut, Egypt

    Reffat M. Abdelal

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  1. Enas A. Hamed
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  2. Madeha M. Zakary
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  3. Reffat M. Abdelal
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  4. Effat M. Abdel Moneim
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Correspondence to Enas A. Hamed.

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Hamed, E.A., Zakary, M.M., Abdelal, R.M. et al. Vasculopathy in type 2 diabetes mellitus: role of specific angiogenic modulators. J Physiol Biochem 67, 339–349 (2011). https://doi.org/10.1007/s13105-011-0080-8

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  • DOI: https://doi.org/10.1007/s13105-011-0080-8

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