Abstract
Virus diseases are the major limiting factors of chilli production in China. A virus isolate of chilli ringspot virus-Yunnan (ChiRSV-YN) from chilli (Capsicum annuum L.) showing mild mosaic, leaf crinkling, ringspot, and vein banding symptoms was recently identified in Yunnan, China. The electron microscopy confirmed the virus as a typical potyvirus having flexuous filamentous particles ca. 780 nm in length. Several chilli and tobacco varieties were tested through mechanical inoculation and found to be susceptible to the virus isolate ChiRSV-YN. The viral genome was sequenced through RNA sequencing. The complete genomic RNA of the isolate (GenBank Acc. No. KX258620) consisted of 9,652 nucleotides (nt) excluding the poly (A) tail at the 3′ end. The isolate contains a large ORF which encodes a polyprotein of 3,086 amino acids (aa) with Mr. of 349.21 kDa. It had a typical genomic organization of potyviruses. The complete nucleotide and polyprotein sequences of ChiRSV-YN had 87.6 and 93.2% identities with the ChiRSV-HN/14, respectively. The result showed ChiRSV-YN is a new isolate of ChiRSV and it is the first full-length genome characterization of ChiRSV in Yunnan, China. Understanding of the characteristics of this virus isolate might provide the basis for disease diagnosis and control in the future.
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Acknowledgements
This work was partially supported by grants from the Provincial Natural Science Foundation of Yunnan (2012CH007), Yunnan Provincial High-Level Talent Introduction Plan (2013HA028), and Yunnan Scholar (Zhongkai Zhang).
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The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be interpreted as a potential conflict of interest.
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Md. Siddiqur Rahman and Su Xiaoxia were involved in the execution of the research work; collection, analysis, and interpretation of the data; manuscript writing, etc., Kuanyu Zheng, Xiaofei Cheng, and Ting Li, Lihua Zhao assisted during the work and editing of the manuscript. Jiahong Dong, Zhongkai Zhang was involved in the research design and planning, analysis, and interpretation of data, critical revision of the work for intellectual content.
The nucleotide sequence data reported in this paper are available in the GenBank database under accession numbers: KX258620
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Rahman, M.S., Su, X., Zheng, K. et al. Characterization of a New Isolate of Chilli ringspot virus in Yunnan, China. J. Crop Sci. Biotechnol. 23, 57–63 (2020). https://doi.org/10.1007/s12892-019-0202-0
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DOI: https://doi.org/10.1007/s12892-019-0202-0