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Genomic sequencing and analysis of Chilli ringspot virus, a novel potyvirus

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Abstract

Chilli ringspot virus (ChiRSV), a novel potyvirus, was recently found in Hainan, China with high prevalence. The genomic sequence of the ChiRSV-HN/14 isolate was determined by sequencing overlapping cDNA segments generated by reverse transcription polymerase chain reaction with degenerate and/or specific primers. ChiRSV genome (GenBank Acc. no. JN008909) comprised of 9,571 nucleotides (nt) excluding the 3′-terminal poly (A) tail and contained a large open reading frame of 9,240 nt encoding a large polyprotein of 3,079 amino acids with predicted Mr of 349.1 kDa. A small, overlapping PIPO coding region was also found to span from nt 2,913 to 3,095, with a capacity to encode a peptide of 60 amino acids. ChiRSV shares sequence identities of only 48.5–65.4 and 42.9–68.7% with closely related potyviruses at the nucleotide and the amino acid levels, respectively. Phylogenetic analysis of the genomic sequences provided further evidence that ChiRSV is a distinct species of the Potyvirus genus. ChiRSV-HN/14 is most closely related to Tobacco vein banding mosaic virus and two other pepper-infecting potyviruses.

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Authors and Affiliations

  1. Key Laboratory for Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agriculture Science, Haikou, Hainan, 571101, People’s Republic of China

    Dian Gong, Jian-Hua Wang, Zhan-Song Lin, Shao-Yan Zhang, Yu-Liang Zhang, Nai-Tong Yu & Zhi-Xin Liu

  2. College of Environment and Plant Protection, Hainan University, Haikou, Hainan, 570228, People’s Republic of China

    Dian Gong, Zhan-Song Lin, Shao-Yan Zhang & Nai-Tong Yu

  3. BIO5 Institute and School of Plant Sciences, University of Arizona, Tucson, AZ, 85721, USA

    Zhongguo Xiong

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  1. Dian Gong
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  2. Jian-Hua Wang
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Correspondence to Zhongguo Xiong or Zhi-Xin Liu.

Additional information

Zhongguo Xiong and Zhi-Xin Liu contributed equally to this research.

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Gong, D., Wang, JH., Lin, ZS. et al. Genomic sequencing and analysis of Chilli ringspot virus, a novel potyvirus. Virus Genes 43, 439–444 (2011). https://doi.org/10.1007/s11262-011-0652-2

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  • DOI: https://doi.org/10.1007/s11262-011-0652-2

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